Secondary Antibodies

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Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258BT-N

USD 680.00

2 Weeks

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Biotin

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Biotin
Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258FC-N

USD 680.00

2 Weeks

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, FITC

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation FITC
Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258PU-N

USD 550.00

2 Weeks

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Purified

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of
cytoplasmic IgG, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1000 upwards.
Reactivities Human
Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258TC-N

USD 680.00

2 Weeks

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, TRITC

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to etermine its concentration in techniques as ELISA, indirect mmunoperoxidase staining of ytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/1-1/50.
ELISA: from 1/20 upwards.
Western blot: from 1/40 upwards.
Reactivities Human
Conjugation TRITC
Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
AM20259AF-N

USD 450.00

2 Weeks

Human IgM mouse monoclonal antibody, clone NI 179, Ascites

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. The optimum working
dilution is an assay-related characteristic and should always be determined by titration.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1,000 upwards.
Reactivities Human
Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
AM20259BT-N

USD 680.00

2 Weeks

Human IgM mouse monoclonal antibody, clone NI 179, Biotin

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. As a second step an Avidin or Streptavidin conjugate have to be used.
General Recommended Dilutions:
Histochemical Use: 1/100-1/500
ELISA: from 1/1,000 upwards
Western blot: from 1/2,000 upwards
Reactivities Human
Conjugation Biotin
Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
AM20259FC-N

USD 680.00

2 Weeks

Human IgM mouse monoclonal antibody, clone NI 179, FITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
Reactivities Human
Conjugation FITC
Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
AM20259PU-N

USD 550.00

2 Weeks

Human IgM mouse monoclonal antibody, clone NI 179, Purified

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, immunoblotting, immunoprecipitation, indirect immunoperoxidase and immunofluorescence
General Recommended Dilutions:
Histochemical use: 1/50-1/200
Dilutions may be prepared by adding PBS, pH 7.2.
Reactivities Human
Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
AM20259TC-N

USD 680.00

2 Weeks

Human IgM mouse monoclonal antibody, clone NI 179, TRITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
Reactivities Human
Conjugation TRITC
Human IgG (Fc heavy chain specific) Mouse Monoclonal Antibody [Clone ID: EM-07]
AM26025HR-N

USD 280.00

2 Weeks

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, HRP

Applications ELISA: 0.1-0.5 µg/ml. 
Western blot.
Reactivities Human
Conjugation HRP
Human IgE Mouse Monoclonal Antibody [Clone ID: 904]
AM31550PU-N

USD 525.00

2 Weeks

Human IgE mouse monoclonal antibody, clone 904, Purified

Applications ELISA.
Reactivities Human
Human IgG Mouse Monoclonal Antibody [Clone ID: B573M]
AM31552PU-N

USD 315.00

2 Weeks

Human IgG mouse monoclonal antibody, clone B573M, Purified

Applications ELISA.
Lateral Flow Assays.
Reactivities Human
Mouse IgG (heavy chain) Rat Monoclonal Antibody [Clone ID: LO-MG-7]
AM32194BT-N

USD 770.00

2 Weeks

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, Biotin, Purified

Applications Immunoassays.
ELISA: good binding on plastic
Immunohistochemistry on paraffin sections. 
Indirect Immunofluorescence on human peropheral blood cells.
Reactivities Mouse
Conjugation Biotin
Mouse IgG (heavy chain) Rat Monoclonal Antibody [Clone ID: LO-MG-7]
AM32194PU-N

USD 630.00

2 Weeks

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, Purified

Applications ELISA (good binding on plastic) 
LO-MG-7 purified can be used at 0.6 µg/ml to obtain an OD of more than 1 on Mouse IgG2a en IgG2b.
Immunoassay: Useful as Secondary antibody.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Human IgM Mouse Monoclonal Antibody [Clone ID: ICL-931]
AM32216HR-N

USD 680.00

2 Weeks

Human IgM mouse monoclonal antibody, clone ICL-931, HRP, Purified

Applications ELISA: 1/1000.
Radioimmunoassay.
Dot Blot: 1/100.
Immunohistochemistry on paraffin sections: 1/40.
Reactivities Human
Conjugation HRP
Human IgA (alpha chain) Goat Polyclonal Antibody
AP00640PU-N

USD 185.00

3 Days

Human IgA (alpha chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/80,000.
Wester Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Human
Immunogen
Human IgA alpha heavy chain.
Rat IgG2a Goat Polyclonal Antibody
AP05379HR-N

USD 310.00

5 Days

Rat IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP
Rat IgG2a Goat Polyclonal Antibody
AP05379PU-N

USD 250.00

5 Days

Rat IgG2a goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Mouse IgG2a Goat Polyclonal Antibody
AP05385HR-N

USD 310.00

5 Days

Mouse IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blotting.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG2a paraproteins
Mouse IgG2b Goat Polyclonal Antibody
AP05386HR-N

USD 310.00

5 Days

Mouse IgG2b goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000. 
Western Blot. 
Immunohistochemistry on Frozen Sections
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG2b paraproteins
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138BT-N

USD 160.00

3 Days

Turkey IgG (H+L chain) goat polyclonal antibody, Biotin

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Conjugation Biotin
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138FC-N

USD 130.00

3 Days

Turkey IgG (H+L chain) goat polyclonal antibody, FITC

Applications Immunofluorescence: < / = 1 µg/10e6 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Conjugation FITC
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138PU-N

USD 120.00

3 Days

Turkey IgG (H+L chain) goat polyclonal antibody, Purified

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138RP-N

USD 380.00

3 Days

Turkey IgG (H+L chain) goat polyclonal antibody, PE

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Conjugation PE
Mouse IgG (H+L chain) Goat Polyclonal Antibody
AP09454PU-N

USD 195.00

3 Days

Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/150,000.
Western Blot: 1/3,000-1/15,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Immunogen Mouse IgG, whole molecule.
Mouse IgG (Fc specific) Goat Polyclonal Antibody
AP20140PU-N

USD 205.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction

Applications ELISA: 1/20000-1/100000. 
Western Blot: 1/2000-1/10000. 
Immunohistochemistry: 1/1000-1/5,000.
Reactivities Mouse
Immunogen Mouse IgG F(c) fragment
Human IgG1 Rabbit Polyclonal Antibody
AP20578PU-N

USD 370.00

2 Weeks

Human IgG1 rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human, Mouse, Rat
Immunogen Synthetic peptide, corresponding to amino acids 211-260 of Human IGHG1.
Human IgG (F(ab)2 specific) Goat Polyclonal Antibody
AP21427FC-N

USD 410.00

2 Weeks

Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80. 
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation FITC
Immunogen Purified Fab prepared from normal IgG isolated from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgG (F(ab)2 specific) Goat Polyclonal Antibody
AP21427TC-N

USD 410.00

2 Weeks

Human IgG (F(ab)2 specific) goat polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified Fab prepared from normal IgG isolated from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgA (Fc specific) Sheep Polyclonal Antibody
AP21428TC-N

USD 410.00

2 Weeks

Human IgA (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified IgA prepared from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgG (Fc specific) Sheep Polyclonal Antibody
AP21429TC-N

USD 410.00

2 Weeks

Human IgG (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified IgG prepared from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482TC-N

USD 410.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/60, depending on the method used.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Porcine IgM (heavy chain) Rabbit Polyclonal Antibody
AP24004PU-N

USD 605.00

3 Days

Porcine IgM (heavy chain) rabbit polyclonal antibody, Purified

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10000-1/50000.
Immunofluorescence: 1/1000-1/5000.
Flow Cytometry: 1/500-1/2500.
Reactivities Porcine
Immunogen Swine IgM mu heavy chain
Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435FC-N

USD 410.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, FITC

Applications Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (H+L chain) Goat Polyclonal Antibody
AP31439FC-N

USD 410.00

2 Weeks

Monkey IgG (H+L chain) goat polyclonal antibody, FITC

Applications To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (H+L chain) Rabbit Polyclonal Antibody
AP31448FC-N

USD 480.00

2 Weeks

Monkey IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgA (Fc specific) Goat Polyclonal Antibody
AP31539FC-N

USD 410.00

2 Weeks

Canine IgA (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80, depending on the method used.
Reactivities Canine
Conjugation FITC
Immunogen Pools of purified IgA isolated from dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (Fc specific) Goat Polyclonal Antibody
AP31540FC-N

USD 410.00

2 Weeks

Canine IgG (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgG isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/15 and 1/60, depending on the method used.
Reactivities Canine
Conjugation FITC
Immunogen Pools of purified IgG isolated from dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541FC-N

USD 480.00

2 Weeks

Canine IgG (H+L chain) goat polyclonal antibody, FITC

Applications Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/120.
Reactivities Canine
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542FC-N

USD 410.00

2 Weeks

Canine IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
General Recommended Dilutions: are usually between 1/10 and 1/40, depending on the method used.
Reactivities Canine
Conjugation FITC
Immunogen Purified IgM isolated from Dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543BT-N

USD 410.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
For histochemical and Cytochemical use are usually between 1/100 and 1/250
In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgM isolated from Mouse serum.
Immunization with intact (19S) and split IgM (7S).
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543FC-N

USD 410.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, FITC

Applications In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Mouse
Conjugation FITC
Immunogen Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split (7S) IgM. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543TC-N

USD 410.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, TRITC

Applications Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/80.
Reactivities Mouse
Conjugation TRITC
Immunogen Purified pools of homogenous IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795PU-N

USD 190.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution).
Western blot (1/5000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Immunoprecipitation (1/250).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Goat IgG (H+L chain) Chicken Polyclonal Antibody
AP31796PU-N

USD 190.00

2 Weeks

Goat IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Goat
Immunogen Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (whole protein).
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277HR-N

USD 230.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277PU-N

USD 200.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified

Applications Immunoblotting.
Flow Cytometry. 
Immunohistochemistry.
Enzyme-Linked-Immunosorbent-Assay (ELISA).
Fluorescent-Linked-Immunosorbent-Assay (FLISA).
Reactivities Mouse
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277RP-N

USD 410.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, PE

Applications FLISA.
Flow Cytometry. 
Immunofluorescence: ≤ 0.1 µg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation PE
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277TC-N

USD 290.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, TRITC

Applications FLISA: 1/100-1/400.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation TRITC
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP32278AP-N

USD 250.00

3 Days

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.