Peroxidase Conjugated Goat Anti-Rabbit IgG (Min Hu, Ms)
Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
Reactivities | Rabbit IgG |
Conjugation | HRP |
Immunogen | Rabbit IgG, whole molecule |
Peroxidase Conjugated Goat Anti-Rabbit IgG (Min Hu, Ms)
Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
Reactivities | Rabbit IgG |
Conjugation | HRP |
Immunogen | Rabbit IgG, whole molecule |
Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]
Applications | WB: 1: 1,000; ICC: 1:200; IP: 1:200 |
Reactivities | Mouse IgG |
Conjugation | HRP |
Immunogen | Mouse IgG, whole molecule |
Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)
Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
Reactivities | Rabbit IgG |
Conjugation | HRP |
Immunogen | Rabbit IgG, whole molecule |
Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)
Applications | Dot blot:0.5-1µg/ml.Western blot(DAB):0.7-3.3µg/ml.Western blot(ECL):0.2-0.5µg/ml.ELISA:0.2-0.3µg/ml. |
Reactivities | Mouse IgG |
Conjugation | HRP |
Immunogen | Mouse IgG, whole molecule |
beta 2 Microglobulin (B2M) rabbit polyclonal antibody, HRP
Applications | ELISA, EM, IHC, WB |
Reactivities | Human |
Conjugation | HRP |
Immunogen | Beta-2-Microglobulin [Human Urine]. |
Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)
Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
Reactivities | Mouse IgG |
Conjugation | HRP |
Immunogen | Mouse IgG, whole molecule |
Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)
Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
Reactivities | Rabbit IgG |
Conjugation | HRP |
Immunogen | Rabbit IgG, whole molecule |
Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)
Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
Reactivities | Goat IgG |
Conjugation | HRP |
Immunogen | Goat IgG, whole molecule |
Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized
Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/150,000-1/250,000. Immunohistochemistry: 1/500-1/2,500. Western blot: 1/10,000-1/40,000. |
Reactivities | Rabbit |
Conjugation | HRP |
Immunogen | Rabbit IgG, whole molecule |
GFP (Ads. to Hu, Ms, Rt Serum Proteins) goat polyclonal antibody, HRP
Applications | ELISA, IHC, WB |
Conjugation | HRP |
Immunogen | GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria |
Human IgM (Mu chain specific) goat polyclonal antibody, HRP
Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions ELISA: 1/80,000. Western blot: 1/2,000-1/20,000. Immunohistochemistry: 1/500-1/2,500. |
Conjugation | HRP |
Immunogen | Human IgM µ (mu) heavy chain |
Human IgG (Fc specific) goat polyclonal antibody, HRP
Applications | ELISA: 1/3000. Immunohistochemistry on Frozen Sections. |
Reactivities | Human |
Conjugation | HRP |
Immunogen | Human IgG |
Human IgG3 sheep polyclonal antibody, HRP
Applications | ELISA. Immunohistochemistry on Frozen Sections: 1/100-1/200. |
Reactivities | Human |
Conjugation | HRP |
Immunogen | Human IgG3 (kappa & lambda) purified from Human myeloma serum |
Candida albicans rabbit polyclonal antibody, HRP
Applications | ELISA, ID, IF, IHC |
Reactivities | Candida |
Conjugation | HRP |
Immunogen | Candida albicans, type A (ATCC #32354) |
Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP
Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA : 1:50,000 - 1:300,000 Western Blot : 1:10,000 - 1:30,000 Immunohistochemistry: 1:500 - 1:2,500 Other Dilution: User Optimized |
Conjugation | HRP |
Immunogen | Mouse IgG whole molecule. |
Mouse IgG (H+L chain) sheep polyclonal antibody, HRP
Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/200,000 Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
Reactivities | Mouse |
Conjugation | HRP |
Immunogen | Mouse IgG whole molecule. |
USD 280.00
2 Weeks
Human IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP
Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/130,000 Western blot: 1/1,000-1/5,000 Immunohistochemistry: 1/500-1/2,500 |
Conjugation | HRP |
Immunogen | Human IgG F(c) fragment. |
Human IgE goat polyclonal antibody, HRP
Applications | ELISA: 1/10000-1/100000. |
Reactivities | Human, Monkey |
Conjugation | HRP |
Immunogen | Native Human IgE |
Borrelia burgdorferi rabbit polyclonal antibody, HRP
Applications | ELISA, IF, IHC, WB |
Conjugation | HRP |
Immunogen | Whole cell preparation from B. burgdorferi. |
Goat IgG (H+L chain) rabbit polyclonal antibody, HRP
Applications | ELISA: 1/50,000-1/100,000. Western Blot: 1/5,000-1/20,000. Immunohistochemistry: 1/1,000-1/5,000. Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. |
Conjugation | HRP |
Immunogen | Goat IgG whole molecule. |
FITC goat polyclonal antibody, HRP
Applications | ELISA, IHC, WB |
Conjugation | HRP |
Immunogen | Fluorescein conjugated to Goat IgG |
Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP
Applications | ELISA: 1/100,000. Western blot: 1/500-1/3,000. Immunohistochemistry: 1/500-1/1,500. |
Conjugation | HRP |
Immunogen | Rat IgG, IgA, IgM whole molecules |
C1QA sheep polyclonal antibody, HRP
Applications | ELISA |
Reactivities | Human |
Conjugation | HRP |
Immunogen | Human C1q Preparation: Antisera to Human C1q were raised by repeated immunisation of sheep with highly purified antigen. |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
Reactivities | Mouse |
Conjugation | HRP |
Immunogen | Purified homogenous IgE isolated from pooled Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP
Applications | ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids). Immunocytochemistry. Immunohistochemistry. Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties. Dot blot. Western blot. General Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen. |
Reactivities | Rabbit |
Conjugation | HRP |
Immunogen | Purified immunoglobulin fractions isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgA + IgG + IgM goat polyclonal antibody, HRP
Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunoprecipitation: 1/1,000-1/5,000. |
Reactivities | Human |
Conjugation | HRP |
Immunogen | Human IgG, IgA and IgM whole molecules in goat. |
Mouse IgG (Fc specific) goat polyclonal antibody, HRP
Applications | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000 Western blot: 1/1,000-1/5,000 Flow Cytometry: 1/500-1/2,500 |
Reactivities | Mouse |
Conjugation | HRP |
Immunogen | Mouse IgG F(c) fragment |
Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)
Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
Reactivities | Rat IgG |
Conjugation | HRP |
Immunogen | Rat IgG, whole molecule |
Human IgA (Secretory component) goat polyclonal antibody, HRP
Applications | In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in human milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/6000. |
Reactivities | Human |
Conjugation | HRP |
Immunogen | Secretory component can be present in Human secretions bound to secretory IgA (sIgA) and in free form. Free Human secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP
Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000. |
Reactivities | Monkey |
Conjugation | HRP |
Immunogen | Purified normal Fab of polyclonal Rhesus Monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
XDH rabbit polyclonal antibody, HRP, Purified
Applications | ELISA, IP, WB |
Reactivities | Bovine |
Conjugation | HRP |
Immunogen | Xanthine oxidase (XDH) from bovine buttermilk |
Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP
Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. Note: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/40,000 of the reconstitution concentration is suggested for this product. |
Conjugation | HRP |
Immunogen | Rabbit IgG whole molecule. |
Duck IgM (Fc specific) goat polyclonal antibody, HRP
Applications | Suitable for use in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of Duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/3,000. Immunohistochemistry and Cytochemistry: 1/50-1/250. Note: This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
Reactivities | Duck |
Conjugation | HRP |
Immunogen | Purified normal IgM isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgE (Fc specific) goat polyclonal antibody, HRP
Applications | Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000. |
Reactivities | Human |
Conjugation | HRP |
Immunogen | Purified monoclonal IgE isolated from Human serum. Feund’s complete adjuvant is used in the first step of the immunization procedure. |
C3 goat polyclonal antibody, HRP
Applications | ELISA, ID, IF, IHC, IP, WB |
Reactivities | Monkey |
Conjugation | HRP |
Immunogen | C3c isolated and purified from pooled normal Rhesus Monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (Fc specific) goat polyclonal antibody, HRP
Applications | Dot blot. Immunoblotting. ELISA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. This antibody can be used: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histo- and Cytochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000. |
Reactivities | Monkey |
Conjugation | HRP |
Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Pigeon IgG (H+L chain) rabbit polyclonal antibody, HRP
Applications | ELISA, IF, IHC, WB |
Reactivities | Pigeon |
Conjugation | HRP |
Immunogen | Purified normal IgG isolated from pooled pigeon serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
HXK1 rabbit polyclonal antibody, HRP
Applications | ELISA, WB |
Reactivities | Yeast |
Conjugation | HRP |
Immunogen | Hexokinase from Yeast |
Monkey IgA + IgG + IgM goat polyclonal antibody, HRP
Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
Conjugation | HRP |
Immunogen | Monkey IgG IgA and IgM whole molecules in goat. |
Ferret IgA (alpha chain specific) goat polyclonal antibody, HRP
Applications | ELISA, IHC, WB |
Reactivities | Ferret |
Conjugation | HRP |
Immunogen | Ferret IgA alpha (alpha chain). |
Porcine IgA goat polyclonal antibody, HRP
Applications | ELISA: 1/10,000-1/100,000. |
Reactivities | Porcine |
Conjugation | HRP |
Immunogen | Purified Porcine IgA |
Fibrinogen goat polyclonal antibody, HRP
Applications | ELISA, ID, IF, IHC, IP, WB |
Reactivities | Canine |
Conjugation | HRP |
Immunogen | Fibrinogen is isolated from fresh plasma after removing Prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure |
C3 goat polyclonal antibody, HRP
Applications | ELISA, ID, IF, IHC, IP, WB |
Reactivities | Rat |
Conjugation | HRP |
Immunogen | C3c isolated and purified from pooled normal Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Chicken IgM (Fc specific) goat polyclonal antibody, HRP
Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. |
Reactivities | Chicken |
Conjugation | HRP |
Immunogen | Purified normal IgM isolated from pooled chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, HRP
Applications | This antibody can be used in Enzyme Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: Histochemical and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,0000. |
Reactivities | Guinea Pig |
Conjugation | HRP |
Immunogen | Purified IgG2 isolated from Guinea Pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgM (Fc specific) goat polyclonal antibody, HRP
Applications | Can be used for Enzyme-Immunocytochemical and immunohistochemical detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Guinea Pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Guinea Pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/400-1/4000. |
Reactivities | Guinea Pig |
Conjugation | HRP |
Immunogen | Purified normal IgM isolated from pooled Guinea Pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Complement C3 (C3) goat polyclonal antibody, HRP
Applications | ELISA, ID, IF, IHC, WB |
Reactivities | Human |
Conjugation | HRP |
Immunogen | C3c is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods. Freund’s complete adjuvant is used in the first step of the immunization. |
Lactoferrin (LTF) goat polyclonal antibody, HRP
Applications | ELISA, ID, IF, IHC, IP, WB |
Reactivities | Human |
Conjugation | HRP |
Immunogen | The immunogen has been isolated from human milk. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgD (Fc specific) goat polyclonal antibody, HRP
Applications | Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in nonisotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/50 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/10,000. |
Reactivities | Human |
Conjugation | HRP |
Immunogen | Purified polyclonal IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgE (Fc specific) goat polyclonal antibody, HRP
Applications | This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
Reactivities | Rat |
Conjugation | HRP |
Immunogen | Purified homogenous IgE isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |