Secondary Antibodies

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Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), HRP

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Purified

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmuno assay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), TRITC

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA, and Immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100.
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation TRITC

Human IgD mouse monoclonal antibody, clone NI 158, Ascites

Applications To identify the presence of IgD in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgD and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/25 upwards.
Western blot: from 1/50 upwards.
Reactivities Human

Human IgD mouse monoclonal antibody, clone NI 158, Biotin

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase
staining of cytoplasmic IgD, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Biotin

Human IgD mouse monoclonal antibody, clone NI 158, FITC

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgD and cell membrane IgD.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
Reactivities Human
Conjugation FITC

Human IgD mouse monoclonal antibody, clone NI 158, HRP

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/500.
ELISA: from 1/1000 upwards.
Western blot: from 1/2000 upwards.
Reactivities Human
Conjugation HRP

Human IgD mouse monoclonal antibody, clone NI 158, Purified

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgD Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
ELISA: from 1/25 upwards.
Western blot: from 1/50 upwards.
Reactivities Human

Human IgE mouse monoclonal antibody, clone NI 307, Ascites

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgE and immunoblotting.
General Recommended Dilutions:
Histochemical 1/10-1/100.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human

Human IgE mouse monoclonal antibody, clone NI 307, Biotin

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgE, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Conjugation Biotin

Human IgE mouse monoclonal antibody, clone NI 307, HRP

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgE mouse monoclonal antibody, clone NI 307, Purified

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgE Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Ascites

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), FITC

Applications To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), Ascites

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), Biotin

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG2, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), FITC

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), HRP

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct Immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/25-1/100.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), Purified

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), TRITC

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Azide Free

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), FITC

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), TRITC

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Ascites

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Biotin

Applications To identify the presence of IgG3 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250. 
ELISA: from 1/1,000 upwards. 
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), FITC

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect Immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation FITC

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), TRITC

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50. 
ELISA: from 1/200 upwards. 
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Ascites

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG4, and Immunoblotting.
General Recommended Dilutions:
Immunohistochemistry: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), FITC

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. 
General Recommended Dilutions:
Histochemical Use: 1/10-1/50. 
ELISA: from 1/200 upwards. 
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), TRITC

Applications The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation TRITC

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Biotin

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Biotin

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, FITC

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation FITC

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Purified

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of
cytoplasmic IgG, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1000 upwards.
Reactivities Human

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, TRITC

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to etermine its concentration in techniques as ELISA, indirect mmunoperoxidase staining of ytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/1-1/50.
ELISA: from 1/20 upwards.
Western blot: from 1/40 upwards.
Reactivities Human
Conjugation TRITC

Human IgM mouse monoclonal antibody, clone NI 179, Ascites

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. The optimum working
dilution is an assay-related characteristic and should always be determined by titration.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1,000 upwards.
Reactivities Human

Human IgM mouse monoclonal antibody, clone NI 179, Biotin

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. As a second step an Avidin or Streptavidin conjugate have to be used.
General Recommended Dilutions:
Histochemical Use: 1/100-1/500
ELISA: from 1/1,000 upwards
Western blot: from 1/2,000 upwards
Reactivities Human
Conjugation Biotin

Human IgM mouse monoclonal antibody, clone NI 179, FITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone NI 179, Purified

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, immunoblotting, immunoprecipitation, indirect immunoperoxidase and immunofluorescence
General Recommended Dilutions:
Histochemical use: 1/50-1/200
Dilutions may be prepared by adding PBS, pH 7.2.
Reactivities Human

Human IgM mouse monoclonal antibody, clone NI 179, TRITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
Reactivities Human
Conjugation TRITC

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, HRP

Applications ELISA: 0.1-0.5 µg/ml. 
Western blot.
Reactivities Human
Conjugation HRP

Human IgE mouse monoclonal antibody, clone 904, Purified

Applications ELISA.
Reactivities Human

Human IgG mouse monoclonal antibody, clone B573M, Purified

Applications ELISA.
Lateral Flow Assays.
Reactivities Human

Human IgM mouse monoclonal antibody, clone ICL-931, HRP, Purified

Applications ELISA: 1/1000.
Radioimmunoassay.
Dot Blot: 1/100.
Immunohistochemistry on paraffin sections: 1/40.
Reactivities Human
Conjugation HRP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Biotin

Applications ELISA: 1/5,000-1/10,000.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Biotin

Rat IgD (heavy chain) mouse monoclonal antibody, clone MARD-3, Purified

Applications Immunohistochemistry on Frozen Sections.
ELISA: This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent.
Reactivities Rat

Canine IgE mouse monoclonal antibody, clone E6-71A1, Purified

Applications ELISA.
Western Blotting: Under reducing conditions, this antibody detects a single major band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivities Canine

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone HP6200, Purified

Applications ELISA.
Western blotting.
Radioimmunoassay.
Immunohistochemistry on Frozen Sections: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. 
Does not work on Paraffin Sections.
Reactivities Human

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified

Applications Western blot.
Flow cytometry: 1µg/ml.
Immunocytochemistry.
ELISA.
Reactivities Human

Biotin Conjugated Mouse Anti-Rabbit IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rabbit IgG
Conjugation Biotin
Immunogen Rabbit IgG, whole molecule