Secondary Antibodies

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Bovine IgA (secretory) Rabbit Polyclonal Antibody
AP21499SU-N

USD 270.00

2 Weeks

Bovine IgA (secretory) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Bovine serum, milk or other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of Bovine IgA from serum or exocrine secretions.
Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA.
This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal Bovine serum in agar block titration.
Reactivities Bovine
Immunogen Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgG Rabbit Polyclonal Antibody
AP31649PU-N

USD 455.00

2 Weeks

Human IgG rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Reactivities Human
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
AP31662PU-N

USD 455.00

2 Weeks

Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Ouchterlony Test:
Sample size: 15 µl.
Distance between wells: 5 mm.
Test Time: 5 days.
Temperature: 37°C.
Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG.
Results:
Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG.
No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution.
Protein content: Biuret Assay 14 mg/ml.
Reactivities Mouse
Duck IgA+IgG+IgM (H+L chain) Rabbit Polyclonal Antibody
AP33056SU-N

USD 270.00

2 Weeks

Duck IgA+IgG+IgM (H+L chain) rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Can be used in immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal Duck serum.
Directions for Use:
In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/64 when tested against normal Duck serum in agar block titration.
Reactivities Duck
Immunogen Purified normal Ig fractions isolated from pooled Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Duck IgG (H+L chain) Rabbit Polyclonal Antibody
AP33057FC-N

USD 480.00

2 Weeks

Duck IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:  1/20-1/80.
Reactivities Duck
Conjugation FITC
Immunogen Purified normal Ig fractions isolated from pooled Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Duck IgG (H+L chain) Rabbit Polyclonal Antibody
AP33057SU-N

USD 180.00

2 Weeks

Duck IgG (H+L chain) rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Can be used in Immunoelectrophoresis and Double Radial Immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal duck serum; to prepare an immunoadsorbent for the production of Ig-free duck serum, plasma or non-Ig preparations.
Directions for Use:
In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/64 when tested against normal Duck serum in agar block titration.
Reactivities Duck
Immunogen Purified normal Ig fractions isolated from pooled Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgG (Fc specific) Rabbit Polyclonal Antibody
AP33081FC-N

USD 550.00

2 Weeks

Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA. 
Immunocytochemistry.  
(In)direct Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgG (H+L chain) Rabbit Polyclonal Antibody
AP33082AF-N

USD 180.00

2 Weeks

Sheep IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA. 
Dot blot.
Immunoblotting.
Indirect Immunofluorescence. 
Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Immunogen Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgM (Fc specific) Rabbit Polyclonal Antibody
AP33083AF-N

USD 470.00

2 Weeks

Sheep IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications ELISA. 
Dot blot.
Immunoblotting.
Indirect Immunofluorescence. 
Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration
before being used.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgM (Fc specific) Rabbit Polyclonal Antibody
AP33083FC-N

USD 550.00

2 Weeks

Sheep IgM (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.
Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgM (H+L chain) Rabbit Polyclonal Antibody
AP33084AF-N

USD 320.00

2 Weeks

Sheep IgM (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA. 
Dot blot.
Immunoblotting.
Indirect Immunofluorescence. 
Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgM (H+L chain) Rabbit Polyclonal Antibody
AP33084FC-N

USD 410.00

2 Weeks

Sheep IgM (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.
Can be used to identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Porcine IgG (H+L chain) Rabbit Polyclonal Antibody
AP33087FC-N

USD 480.00

2 Weeks

Porcine IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA. 
Immunocytochemistry. 
Immunofluorescence.
Immunohistochemistry Frozen Sections.
Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of swine origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Porcine
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Porcine IgG (H+L chain) Rabbit Polyclonal Antibody
AP33087TC-N

USD 480.00

2 Weeks

Porcine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications ELISA. 
Immunocytochemistry. 
Immunofluorescence.
Immunohistochemistry Frozen Sections.
Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-colour staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Porcine
Conjugation TRITC
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Porcine IgA (secretory) Rabbit Polyclonal Antibody
AP33089SU-N

USD 270.00

2 Weeks

Porcine IgA (secretory) rabbit polyclonal antibody, Serum

Applications Precipitation.
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in swine milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or exocrine secretions. Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/32 when tested against pooled normal swine serum in agar-block immunodiffusion titration.
Reactivities Porcine
Immunogen Highly purified secretory IgA isolated from pooled Swine milk.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253AP

USD 270.00

3 Days

Mouse IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Mouse IgG whole molecule
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253B

USD 240.00

3 Days

Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation Biotin
Immunogen Mouse IgG whole molecule.
Chicken IgG / Chicken IgY (H+L chain) Rabbit Polyclonal Antibody
R1300HRP

USD 255.00

3 Days

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
Western blot: 1/1,000-1/10,000.
ELISA: 1/10,000-1/50,000.
Immunohistochemistry: 1/500-1/2500.
Conjugation HRP
Immunogen Chicken IgY/IgG whole molecule
Bovine IgG (Fc specific) Rabbit Polyclonal Antibody
R1305P

USD 240.00

3 Days

Bovine IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications ELISA: 1:20,000 - 1:100,000.
Immunohistochemistry: 1:1,000 - 1,5,000.
Western blotting: 1:2,000 - 1;10,000. 
Suitable for most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reactivities Bovine
Immunogen Bovine IgG F(c) fragment.
Canine IgG (Fc specific) Rabbit Polyclonal Antibody
R1311HRP

USD 315.00

3 Days

Canine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Dog IgG F(c) fragment
Goat IgG (H+L chain) Rabbit Polyclonal Antibody
R1317B

USD 220.00

3 Days

Goat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/1,00,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation Biotin
Immunogen Goat IgG whole molecule.
Goat IgG (H+L chain) Rabbit Polyclonal Antibody
R1317P

USD 205.00

3 Days

Goat IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/800,000. 
Western blot: 1/10,000-1/60,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Immunogen Goat IgG whole molecule
Goat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
R1318T

USD 250.00

3 Days

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for ELISA, Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA:  1/10,000 - 1/50,000.
IF Microscopy:  1/1000 - 1/5000.
Conjugation TRITC
Immunogen Goat IgG whole molecule.
Goat IgG (Fc specific) Rabbit Polyclonal Antibody
R1319P

USD 210.00

3 Days

Goat IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications ELISA:1/20,000-1/100,000.
Immunohistochemistry:1/1,000-5,000.
WB:1/2,000-10,000.
Immunogen Goat IgG F(c) fragment.
Goat IgG (F(ab)2 specific), adsorbed Rabbit Polyclonal Antibody
R1321P

USD 235.00

3 Days

Goat IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, Aff - Purified

Applications ELISA: 1/20,000-1/100,000.
Immunohistochemistry: 1/1,000-1/5,000.
WB: 1/2,000-1/10,000.
Immunogen Goat IgG F(ab')2 fragment.
Guinea pig IgG (H+L chain) Rabbit Polyclonal Antibody
R1322F

USD 230.00

3 Days

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Conjugation FITC
Immunogen Guinea Pig IgG whole molecule.
Guinea pig IgG (H+L chain) Rabbit Polyclonal Antibody
R1322T

USD 220.00

3 Days

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation TRITC
Immunogen Guinea Pig IgG whole molecule.
Human IgG (H+L chain) Rabbit Polyclonal Antibody
R1333HRP

USD 240.00

3 Days

Human IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000
Western Blot: 1/5,000-1/25,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation HRP
Immunogen Human IgG whole molecule
Human IgG (gamma chain specific) Rabbit Polyclonal Antibody
R1336F

USD 265.00

3 Days

Human IgG (gamma chain specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Human IgG γ gamma chain
Human IgG (F(ab)2 specific) Rabbit Polyclonal Antibody
R1338HRP

USD 300.00

3 Days

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. 
Recommended Dilutions: 
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-2,500.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Human IgG F(ab')2 fragment
Mouse IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
R1350P

USD 235.00

3 Days

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. 
ELISA : 1:20,000 - 1:40,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse
Immunogen Mouse IgG whole molecule.
Mouse IgG1 (gamma 1 chain specific) Rabbit Polyclonal Antibody
R1359HRP

USD 340.00

3 Days

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, HRP

Applications Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays.
Recommended Dilutions:
ELISA: 1/300,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG1 gamma 1 heavy chain
Mouse IgG2a (gamma 2a chain specific) Rabbit Polyclonal Antibody
R1360HRP

USD 340.00

3 Days

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP

Applications This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays.
Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/90,000
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Mouse IgG2a heavy chain
Porcine IgG (H+L chain) Rabbit Polyclonal Antibody
R1384HRP

USD 255.00

3 Days

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Swine IgG whole molecule
Mouse IgG (H+L chain), F(ab)2 Fragment Rabbit Polyclonal Antibody
SP1012B

USD 465.00

5 Days

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified mouse IgG
Canine IgG2 Rabbit Polyclonal Antibody
SP2131HRP

USD 310.00

5 Days

Canine IgG2 rabbit polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Immunohistochemistry on frozen sections: 1:200 - 1:500.
Reactivities Canine
Conjugation HRP
Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)
TA130039

USD 425.00

3 Weeks

Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rat IgG
Conjugation HRP
Immunogen Rat IgG, whole molecule
Mouse IgA Rabbit Polyclonal Antibody
AP05389PU-N

USD 340.00

5 Days

Mouse IgA rabbit polyclonal antibody, Purified

Applications ELISA.
Reactivities Mouse, Rat
Immunogen Mouse IgA paraproteins
Goat IgM Rabbit Polyclonal Antibody
AP05549PU-N

USD 250.00

5 Days

Goat IgM rabbit polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Goat
Immunogen Purified goat IgM
Human IgM Rabbit Polyclonal Antibody
AP10433PU-N

USD 290.00

5 Days

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
Use Polymer anti Mouse/Rabbit immunoglobulin as a detection system.
Positive Control: Lymph node.
Reactivities Human
Immunogen Purified IgM.
Human IgA Rabbit Polyclonal Antibody
AP20644PU-N

USD 370.00

2 Weeks

Human IgA rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human
Rat IgE (Fc specific) Rabbit Polyclonal Antibody
AP21484SU-N

USD 270.00

2 Weeks

Rat IgE (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of homogenous IgE in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgE from serum or plasma.
Recommended dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Rat
Immunogen Pools of purified homogenous IgE isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485SU-N

USD 270.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of homogenous IgE in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgE from serum or plasma.
Recommended dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Mouse
Immunogen Pools of purified homogenous IgE isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Bovine IgG2 (subclass specific) Rabbit Polyclonal Antibody
AP21496SU-N

USD 270.00

2 Weeks

Bovine IgG2 (subclass specific) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in Bovine serum and other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of bovine IgG2 from serum or plasma.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal Bovine serum in agar block titration.
Reactivities Bovine
Immunogen Pools of purified normal IgG2 isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Bovine IgM (Fc specific) Rabbit Polyclonal Antibody
AP21498SU-N

USD 270.00

2 Weeks

Bovine IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in Bovine serum and other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of Bovine IgM from serum or plasma.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/16 when tested against normal Bovine serum in agar block titration.
Reactivities Bovine
Immunogen Highly purified normal IgM isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Feline IgA (Fc specific) Rabbit Polyclonal Antibody
AP21500SU-N

USD 270.00

2 Weeks

Feline IgA (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Cat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of cat IgA from serum or plasma.
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially) diluted in 2 mm diameter peripheral wells.
Antibody titre: 1/32 (Precipitin titre) when tested against pooled normal Cat serum in agar-block immunodiffusion titration.
Reactivities Feline
Immunogen Highly purified normal IgA isolated from pooled Cat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgM (Fc specific) Rabbit Polyclonal Antibody
AP31441SU-N

USD 270.00

2 Weeks

Monkey IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in monkey serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fab specific) Rabbit Polyclonal Antibody
AP31444SU-N

USD 270.00

2 Weeks

Monkey IgG (Fab specific) rabbit polyclonal antibody, Serum

Applications Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.
Recommended working dilutions:
Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified Fab of normal Rhesus monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Fc specific) Rabbit Polyclonal Antibody
AP31445SU-N

USD 270.00

2 Weeks

Monkey IgA (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Working Dilutions:
Immunoelectrophoresis: 2 μl serum or equivalent against 120 μl antiserum.
Double Radial Immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgA isolated from Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fc specific) Rabbit Polyclonal Antibody
AP31446SU-N

USD 270.00

2 Weeks

Monkey IgG (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgG from serum or plasma.
Recommended working dilutions:
Immunoelectrophoresis: 2 μl serum or equivalent against 120 μl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.