IVa2 (AC_000017) Virus Tagged ORF Clone

CAT#: VC100005

Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 1], codon optimized for human cell expression, AP_000502

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100005 represents NCBI reference of AP_000502 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACACGGGGTCGAAGGCCTGCGGCGCTGCAGCACCAGCAAGACCAGCCTCAAGCACACCCAGGCC
  AACGCGCCGCACGCAGTGCTCCACTGCACAGAGACCCTGACTATGCAGATGAAGATCCTGCTCCCGTGGA
  AAGACACGATCCTGGCCCGTCAGGCCGAGCTCCTACTACTGCAGTACAGAGAAAGCCCCCACAGCCAGCA
  AAGCGCGGAGACATGCTGGATCGAGACGCTGTGGAACATGTGACGGAGCTGTGGGACAGGCTCGAACTGC
  TTGGCCAGACGTTGAAAAGTATGCCTACGGCCGACGGATTGAAGCCCCTGAAGAATTTTGCCTCACTCCA
  AGAACTGTTGTCACTGGGGGGCGAGCGGTTGCTGGCTGATCTTGTGAGAGAAAATATGCGAGTGAGGGAT
  ATGCTGAATGAGGTGGCGCCTCTCCTGAGAGACGACGGATCATGCTCTAGTCTTAACTACCAATTGCAGC
  CCGTGATTGGGGTAATCTATGGCCCAACCGGCTGTGGTAAATCCCAGCTGCTGCGCAATCTCCTTAGTTC
  ACAGCTGATCTCACCTACACCCGAGACGGTCTTCTTTATTGCACCACAGGTGGATATGATTCCGCCCAGC
  GAACTGAAGGCTTGGGAAATGCAGATTTGCGAAGGAAACTACGCTCCTGGACCAGACGGAACCATCATTC
  CCCAGTCAGGCACTCTTCGGCCCAGATTTGTGAAAATGGCCTACGACGACCTGATACTCGAACATAATTA
  CGATGTGAGTGATCCCCGCAACATCTTCGCCCAGGCCGCCGCTCGAGGCCCAATCGCCATCATAATGGAC
  GAGTGTATGGAAAACCTGGGCGGCCATAAAGGGGTCTCAAAGTTCTTTCACGCCTTTCCCAGCAAACTCC
  ACGACAAATTTCCTAAGTGCACCGGGTACACAGTTCTGGTGGTCTTGCACAACATGAATCCACGGCGAGA
  CATGGCCGGTAACATCGCAAACCTCAAGATCCAGTCAAAAATGCATCTGATCTCCCCCAGAATGCACCCA
  TCTCAGCTTAATCGGTTCGTGAACACTTATACTAAGGGACTTCCCCTTGCTATTAGTCTCTTGCTGAAGG
  ACATTTTTCGACATCACGCTCAGCGATCATGTTATGATTGGATCATCTATAATACCACCCCTCAGCACGA
  GGCACTGCAGTGGTGTTATCTTCACCCTCGCGACGGGCTGATGCCAATGTATCTGAATATCCAATCACAT
  CTCTACCACGTGCTGGAAAAAATTCACCGAACCTTGAACGATAGGGATCGCTGGAGTAGGGCATACCGCG
  CACGAAAGACTCCTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100005 representing AP_000502
  Red=Cloning sites Green=Tags
  
  METRGRRPAALQHQQDQPQAHPGQRAARSAPLHRDPDYADEDPAPVERHDPGPSGRAPTTAVQRKPPQPA
  KRGDMLDRDAVEHVTELWDRLELLGQTLKSMPTADGLKPLKNFASLQELLSLGGERLLADLVRENMRVRD
  MLNEVAPLLRDDGSCSSLNYQLQPVIGVIYGPTGCGKSQLLRNLLSSQLISPTPETVFFIAPQVDMIPPS
  ELKAWEMQICEGNYAPGPDGTIIPQSGTLRPRFVKMAYDDLILEHNYDVSDPRNIFAQAAARGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDMAGNIANLKIQSKMHLISPRMHP
  SQLNRFVNTYTKGLPLAISLLLKDIFRHHAQRSCYDWIIYNTTPQHEALQWCYLHPRDGLMPMYLNIQSH
  LYHVLEKIHRTLNDRDRWSRAYRARKTPK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000502
• RefSeq ORF: 1347
• MW: 50.9 kDa