pVIII (AC_000017) Virus Tagged ORF Clone

CAT#: VC100021

Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 1], codon optimized for human cell expression, AP_000518

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100021 represents NCBI reference of AP_000518 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAAGAAATCCCCACACCTTATATGTGGTCATATCAGCCGCAGATGGGACTGGCTGCCGGGGCAG
  CCCAGGATTATTCAACCCGCATCAACTACATGTCAGCTGGCCCTCACATGATTAGCCGCGTTAATGGTAT
  CAGAGCCCACAGGAACAGAATACTGCTCGAACAGGCCGCCATAACCACTACTCCTCGCAATAATCTCAAC
  CCCAGAAGCTGGCCCGCGGCCCTCGTCTACCAGGAGTCTCCAGCTCCCACCACCGTGGTGCTGCCTCGCG
  ATGCTCAAGCGGAGGTTCAAATGACCAACAGTGGTGCGCAACTCGCGGGGGGTTTCCGCCACAGGGTGCG
  CTCCCCCGGACAAGGGATTACTCACCTTAAAATAAGGGGAAGGGGCATCCAGCTCAACGACGAATCTGTG
  TCTAGCAGTCTTGGCCTGCGGCCAGATGGAACATTCCAGATAGGAGGCGCTGGAAGATCTTCTTTCACCC
  CAAGACAGGCCATCCTGACTCTGCAGACCAGCAGTAGTGAACCACGGTCTGGAGGCATCGGAACACTGCA
  GTTCATCGAAGAATTCGTGCCCTCTGTGTACTTTAACCCATTCTCCGGGCCGCCTGGACATTATCCTGAT
  CAGTTCATCCCTAACTTTGATGCCGTGAAAGATTCAGCGGATGGCTATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100021 representing AP_000518
  Red=Cloning sites Green=Tags
  
  MSKEIPTPYMWSYQPQMGLAAGAAQDYSTRINYMSAGPHMISRVNGIRAHRNRILLEQAAITTTPRNNLN
  PRSWPAALVYQESPAPTTVVLPRDAQAEVQMTNSGAQLAGGFRHRVRSPGQGITHLKIRGRGIQLNDESV
  SSSLGLRPDGTFQIGGAGRSSFTPRQAILTLQTSSSEPRSGGIGTLQFIEEFVPSVYFNPFSGPPGHYPD
  QFIPNFDAVKDSADGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000518
• RefSeq ORF: 681
• MW: 24.7 kDa