III (AC_000017) Virus Tagged ORF Clone

CAT#: VC100010

Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 1], codon optimized for human cell expression, AP_000507

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100010 represents NCBI reference of AP_000507 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGAGCGGCGATGTATGAAGAAGGACCTCCACCCTCCTACGAGTCCGTGGTGAGCGCCGCACCCG
  TGGCAGCTGCTTTGGGCAGCCCATTCGATGCCCCACTGGACCCCCCATTCGTGCCGCCCAGATACCTTAG
  ACCGACCGGAGGACGCAACTCCATCAGGTACTCTGAACTCGCCCCGCTTTTCGACACAACCAGGGTGTAC
  CTGGTGGATAATAAGTCAACAGATGTGGCAAGCCTGAATTACCAGAACGACCATAGTAATTTTTTGACCA
  CTGTGATACAGAATAATGACTACTCTCCCGGCGAGGCTTCAACACAGACAATCAACCTCGATGATCGATC
  CCACTGGGGCGGCGATCTGAAGACTATACTCCATACTAATATGCCAAACGTTAACGAATTTATGTTCACC
  AATAAGTTCAAAGCCCGGGTCATGGTATCCCGGTCCCTTACTAAAGACAAGCAAGTGGAACTGAAATACG
  AGTGGGTGGAGTTCACCCTGCCTGAGGGCAATTACTCCGAGACCATGACTATAGATCTGATGAATAACGC
  AATTGTGGAACATTATCTCAAAGTCGGGCGCCAGAATGGTGTTCTCGAATCCGACATTGGAGTTAAATTT
  GACACCCGCAATTTCCGGCTGGGCTTTGATCCGGTGACGGGTCTGGTGATGCCCGGCGTGTATACAAATG
  AGGCATTCCATCCCGACATAATTTTGCTGCCTGGCTGCGGCGTCGACTTCACCCACTCAAGACTGAGCAA
  CCTTCTTGGGATCCGGAAACGGCAGCCGTTCCAGGAAGGCTTTAGAATTACGTACGACGACTTGGAAGGT
  GGCAACATCCCTGCCTTGCTCGATGTGGATGCCTATCAAGCCAGCCTCAAAGATGACACTGAGCAGGGGG
  GCGGGGGTGCAGGTGGCGGGAATAACTCTGGATCAGGCGCGGAAGAGAACTCAAATGCCGCTGCCGCTGC
  TATGCAACCTGTTGAGGACATGAACGACCACGCCATACGAGGAGACACCTTTGCCACAAGAGCTGAGGAA
  AAAAGGGCCGAGGCAGAGGCAGCCGCTGAAGCCGCTGCCCCTGCAGCGGAGGCCGCCCAGCCAGAAGTCG
  AGAAACCGCAGAAAAAGCCTGTCATTAAGCCATTGACGGAGGACAGCAAGAAGAGATCCTACAACCTGAT
  CTCCAACGACTCTACTTTCACACAGTATAGATCATGGTATCTGGCTTATAATTACGGCGATCCTCAGGCA
  GGTATTCGGAGTTGGACACTTCTGTGTACCCCCGATGTAACATGCGGATCTGAACAGGTGTACTGGAGTC
  TGCCAGATATGATGCAGGACCCCGTGACATTCCGCTCTACCAGTCAGATCTCAAATTTTCCCGTAGTAGG
  GGCCGAGCTGCTGCCAGTGCACTCAAAGTCTTTCTACAATGACCAAGCAGTGTACTCCCAGCTGATACGC
  CAGTTTACCTCATTGACTCACGTCTTTAACCGGTTCCCCGAGAATCAGATCCTGGCGCGCCCTCCAGCTC
  CTACGATTACTACAGTTTCAGAAAACGTGCCAGCTCTCACCGATCACGGTACCCTCCCACTGCGCAATTC
  CATCGGGGGAGTTCAGCGCGTTACCATTACGGACGCTCGCAGGAGGACCTGTCCTTACGTATACAAAGCT
  CTTGGGATCGTGAGCCCGCGGGTGTTGAGCAGTCGAACCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100010 representing AP_000507
  Red=Cloning sites Green=Tags
  
  MRRAAMYEEGPPPSYESVVSAAPVAAALGSPFDAPLDPPFVPPRYLRPTGGRNSIRYSELAPLFDTTRVY
  LVDNKSTDVASLNYQNDHSNFLTTVIQNNDYSPGEASTQTINLDDRSHWGGDLKTILHTNMPNVNEFMFT
  NKFKARVMVSRSLTKDKQVELKYEWVEFTLPEGNYSETMTIDLMNNAIVEHYLKVGRQNGVLESDIGVKF
  DTRNFRLGFDPVTGLVMPGVYTNEAFHPDIILLPGCGVDFTHSRLSNLLGIRKRQPFQEGFRITYDDLEG
  GNIPALLDVDAYQASLKDDTEQGGGGAGGGNNSGSGAEENSNAAAAAMQPVEDMNDHAIRGDTFATRAEE
  KRAEAEAAAEAAAPAAEAAQPEVEKPQKKPVIKPLTEDSKKRSYNLISNDSTFTQYRSWYLAYNYGDPQA
  GIRSWTLLCTPDVTCGSEQVYWSLPDMMQDPVTFRSTSQISNFPVVGAELLPVHSKSFYNDQAVYSQLIR
  QFTSLTHVFNRFPENQILARPPAPTITTVSENVPALTDHGTLPLRNSIGGVQRVTITDARRRTCPYVYKA
  LGIVSPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000507
• RefSeq ORF: 1722
• MW: 63.5 kDa