22K (AC_000017) Virus Tagged ORF Clone

CAT#: VC100020

Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 1], codon optimized for human cell expression, AP_000517

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 22K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100020 represents NCBI reference of AP_000517 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCAAAGAAAAAGCTCCAGCTCCCGCCACCCCCAACGGACGAAGAAGAGTACTGGGACAGCCAAG
  CCGAAGAAGTGCTTGATGAGGAGGAGGAGGATATGATGGAGGATTGGGAGAGCCTGGACGAAGAAGCGTC
  CGAGGCAGAAGAAGTCTCCGATGAAACACCCTCTCCAAGCGTAGCTTTTCCCTCTCCAGCTCCCCAGAAG
  TCCGCCACAGGCCCCTCCATGGCGACAACCTCAGCTCCTCAAGCTCCACCCGCACTGCCCGTGCGCAGAC
  CCAACAGACGCTGGGACACTACTGGGACCCGGGCCGGAAAGTCCAAGCAGCCACCACCCCTGGCTCAGGA
  ACAACAGCAAAGACAGGGGTACCGGAGCTGGCGCGGCCACAAAAACGCTATCGTCGCTTGTTTGCAGGAT
  TGCGGAGGTAATATTAGCTTCGCTCGCCGGTTCCTGCTCTATCACCACGGGGTCGCTTTTCCACGCAATA
  TACTCCACTACTACCGGCACCTCTACAGTCCATACTGTACGGGCGGGTCCGGCAGCAACTCTTCAGGCCA
  CACCGAGGCTAAGGCTACTGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100020 representing AP_000517
  Red=Cloning sites Green=Tags
  
  MAPKKKLQLPPPPTDEEEYWDSQAEEVLDEEEEDMMEDWESLDEEASEAEEVSDETPSPSVAFPSPAPQK
  SATGPSMATTSAPQAPPALPVRRPNRRWDTTGTRAGKSKQPPPLAQEQQQRQGYRSWRGHKNAIVACLQD
  CGGNISFARRFLLYHHGVAFPRNILHYYRHLYSPYCTGGSGSNSSGHTEAKATG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 582 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000517
• RefSeq ORF: 582
• MW: 21.5 kDa