E3 14.7K (AC_000017) Virus Tagged ORF Clone

CAT#: VC100027

Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus 1], codon optimized for human cell expression, AP_000525

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 14.7K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100027 represents NCBI reference of AP_000525 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGACGGCCTCGATTTGGAGATGGATGGTATAATCACTGAGCAGAGGTTGTTGCAGCGGCGGAAGG
  CTGCGGCGGAGCAGGAGCGACTGAACCAGGAGCTGCAAGACATGGTGAATCTCCACCAGTGTAAGAGGGG
  CATCTTCTGTCTTGTTAAACAAGCCAAGGTGACCTACGAGAAAGATCACTCTAACCATAGACTGAGTTAT
  AAACTCCCTGCCAAACGACAGAAGTTGCTTTTGATGGTTGGCGAGAGGCCAATTACCGTGACCCAGCAGT
  CCGTTGAGACAGAAGGGTGCCTGCATTTCCCATGCCAGGGGCCGGAGGACCTCTGCACTCTCATTAAGAC
  TCTGTGCGGGCTTAGAGACCTCATCCCATTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100027 representing AP_000525
  Red=Cloning sites Green=Tags
  
  MTDGLDLEMDGIITEQRLLQRRKAAAEQERLNQELQDMVNLHQCKRGIFCLVKQAKVTYEKDHSNHRLSY
  KLPAKRQKLLLMVGERPITVTQQSVETEGCLHFPCQGPEDLCTLIKTLCGLRDLIPFN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 384 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000525
• RefSeq ORF: 384
• MW: 14.7 kDa