hexon (AC_000017) Virus Tagged ORF Clone

CAT#: VC100015

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 1], codon optimized for human cell expression, AP_000512

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hexon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100015 represents NCBI reference of AP_000512 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTCCTTCCATGATGCCACAATGGTCTTATATGCACATCTCCGGACAGGATGCATCTGAGTATT
  TGTCCCCAGGACTGGTGCAGTTCGCCAGGGCCACAGAGACATATTTCTCTCTGAACAATAAGTTCAGGAA
  CCCTACCGTGGCTCCTACCCACGATGTGACTACCGACCGATCTCAGAGACTCACCTTGCGCTTTATTCCT
  GTGGATCGAGAGGACACCGCGTACTCCTACAAAGCCCGCTTTACTTTGGCAGTAGGCGACAATAGAGTTC
  TGGATATGGCCAGTACATATTTTGATATTAGAGGCGTCCTGGACCGCGGACCTACCTTCAAGCCTTACAG
  CGGCACAGCCTATAACGCGCTGGCCCCTAAAGGCGCACCTAACTCCTGCGAGTGGGAGCAGGAGGAGCCA
  ACACAGGAAATGGCAGAGGAGCTCGAGGACGAAGAGGAGGCAGAAGAAGAGGAGGCTGAGGAGGAGGCCG
  AAGCGCCCCAGGCCGACCAGAAGGTCAAGAAAACCCATGTATACGCACAGGCGCCCCTGGCCGGAGAGAA
  GATTACGGCCAATGGGCTGCAGATAGTCTCCGACACCCAGACCGAAGGAAATCCCGTGTTCGCCGATCCG
  ACTTATCAGCCTGAGCCGCAGGTTGGTGAAAGCCAGTGGAATGAAGCCGAAGCTACAGCCAGTGGGGGCC
  GCGTCTTGAAGAAGACAACTCCGATGAAGCCTTGCTACGGTTCCTACGCCCGGCCAACAAACAAGAATGG
  AGGCCAAGGTATCCTGGTGGCCAACAACCAAGGCGCTCTGGAGAGCAAAGTGGAGATGCAATTCTTTGCC
  CCGTCCGGCACTGCTATGAACGAAAGAAACGCCGTACAGCCCAGCATTGTTCTCTATAGCGAAGACGTAA
  ATATGGAGACACCTGATACACACATCTCTTACAAGCCCTCAAAAACCGATGAAAACTCCAAGGCCATGCT
  GGGGCAACAGGCAATGCCTAATAGACCCAACTACATCGCCTTTAGAGACAACTTCATCGGGCTCATGTAT
  TACAATTCCACCGGTAACATGGGGGTGCTCGCTGGACAAGCATCCCAACTGAACGCAGTTGTTGATCTTC
  AGGACAGAAACACAGAGCTGTCCTATCAGCTGCTGCTTGATTCCATCGGCGATCGGACACGCTATTTCTC
  CATGTGGAATCAAGCAGTGGATAGTTATGACCCAGATGTGAGAATCATCGAAAATCACGGAACGGAGGAT
  GAACTGCCTAACTACTGCTTCCCACTGGGCGGAATCGGGGTTACCGACACCTATCAGGGGATTAAGAGTA
  ATGGTAATGGGAACCCCCAGAATTGGACCAAAAACGACGACTTCGCAGCACGCAACGAGATTGGTGTCGG
  CAATAACTTCGCCCTCGAAATCAATCTGAACGCCAACCTGTGGCGCAATTTTTTGTATAGCAACATCGCC
  CTGTACCTTCCTGACAAGCTTAAATATACGCCAACGAACGTAGAAATAAGCCCCAATCCAAATTCATATG
  ACTATATGAACAAGAGGGTGGTGGCACCCGGTCTCGTGGATTGCTACATCAATCTGGGTGCTAGGTGGAG
  CCTTGACTATATGGATAATGTCAACCCTTTCAACCACCATCGAAATGCCGGCCTCAGATATAGATCTATG
  CTGCTGGGCAACGGCCGCTATGTTCCATTCCATATACAGGTGCCTCAGAAGTTCTTTGCCATCAAGAACC
  TCCTGCTGCTGCCAGGTTCATACACCTATGAGTGGAATTTCAGGAAGGACGTTAACATGGTCCTCCAATC
  CAGCTTGGGCAACGACCTGAGGGTGGATGGGGCGTCTATCAAGTTCGATAGCATTTGTCTGTACGCAACT
  TTCTTCCCTATGGCCCACAATACTGCCAGCACCCTCGAGGCTATGCTGAGGAATGATACAAATGATCAGT
  CCTTTAATGATTATTTGTCCGCCGCCAATATGCTCTATCCAATTCCTGCTAATGCCACAAATGTCCCTAT
  CTCCATACCAAGTCGAAACTGGGCCGCTTTTCGCGGGTGGGCTTTCACTCGCCTGAAGACCAAAGAGACC
  CCATCCCTGGGCTCTGGGTATGATCCCTATTACACATATTCAGGGTCCATTCCTTACCTGGACGGCACTT
  TCTACCTGAACCACACATTTAAGAAGGTAGCCATAACCTTCGACAGCAGCGTGTCCTGGCCTGGCAACGA
  CCGACTGCTTACCCCCAATGAATTCGAAATTAAGAGGTCCGTGGATGGCGAGGGATACAATGTTGCTCAG
  TGCAATATGACTAAAGACTGGTTCCTGGTCCAAATGCTGGCTAACTACAATATCGGCTACCAAGGGTTCT
  ATATCCCTGAATCCTACAAAGATCGCATGTACAGCTTCTTTCGAAACTTTCAGCCCATGTCTCGACAGGT
  TGTGGACGATACTAAATATAAAGATTACCAGCAGGTCGGTATTCTGCATCAGCATAATAACAGCGGGTTT
  GTGGGATATCTCGCCCCAACTATGCGGGAAGGCCAGGCCTACCCCGCTAATTTCCCTTATCCTCTTATCG
  GCAAGACCGCTGTCGATAGCATAACTCAGAAGAAGTTCCTGTGCGACAGAACACTCTGGCGCATCCCATT
  TTCCTCCAACTTTATGAGCATGGGGGCTCTCACTGACTTGGGACAGAACCTGTTGTATGCCAACAGCGCT
  CACGCACTGGACATGACTTTCGAGGTGGACCCGATGGATGAGCCCACCCTCCTGTATGTGCTGTTTGAAG
  TGTTTGATGTGGTGCGCGTCCATCAGCCTCATCGCGGCGTGATAGAGACGGTGTACCTGAGGACCCCATT
  CTCTGCAGGAAACGCCACCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100015 representing AP_000512
  Red=Cloning sites Green=Tags
  
  MATPSMMPQWSYMHISGQDASEYLSPGLVQFARATETYFSLNNKFRNPTVAPTHDVTTDRSQRLTLRFIP
  VDREDTAYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPTFKPYSGTAYNALAPKGAPNSCEWEQEEP
  TQEMAEELEDEEEAEEEEAEEEAEAPQADQKVKKTHVYAQAPLAGEKITANGLQIVSDTQTEGNPVFADP
  TYQPEPQVGESQWNEAEATASGGRVLKKTTPMKPCYGSYARPTNKNGGQGILVANNQGALESKVEMQFFA
  PSGTAMNERNAVQPSIVLYSEDVNMETPDTHISYKPSKTDENSKAMLGQQAMPNRPNYIAFRDNFIGLMY
  YNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLLLDSIGDRTRYFSMWNQAVDSYDPDVRIIENHGTED
  ELPNYCFPLGGIGVTDTYQGIKSNGNGNPQNWTKNDDFAARNEIGVGNNFALEINLNANLWRNFLYSNIA
  LYLPDKLKYTPTNVEISPNPNSYDYMNKRVVAPGLVDCYINLGARWSLDYMDNVNPFNHHRNAGLRYRSM
  LLGNGRYVPFHIQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASIKFDSICLYAT
  FFPMAHNTASTLEAMLRNDTNDQSFNDYLSAANMLYPIPANATNVPISIPSRNWAAFRGWAFTRLKTKET
  PSLGSGYDPYYTYSGSIPYLDGTFYLNHTFKKVAITFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQ
  CNMTKDWFLVQMLANYNIGYQGFYIPESYKDRMYSFFRNFQPMSRQVVDDTKYKDYQQVGILHQHNNSGF
  VGYLAPTMREGQAYPANFPYPLIGKTAVDSITQKKFLCDRTLWRIPFSSNFMSMGALTDLGQNLLYANSA
  HALDMTFEVDPMDEPTLLYVLFEVFDVVRVHQPHRGVIETVYLRTPFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 2892 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000512
• RefSeq ORF: 2892
• MW: 108.8 kDa