pIIIa (AC_000017) Virus Tagged ORF Clone

CAT#: VC100009

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 1], codon optimized for human cell expression, AP_000506

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pIIIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100009 represents NCBI reference of AP_000506 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAGGATGCTACAGACCCAGCGGTTCGAGCCGCTTTGCAGTCTCAGCCAAGTGGCCTTAATAGCA
  CTGATGACTGGAGGCAGGTTATGGATAGGATCATGTCTCTCACCGCAAGAAACCCAGACGCCTTCAGACA
  GCAGCCCCAAGCAAATAGACTGAGTGCGATCCTGGAGGCTGTGGTCCCTGCGAGGGCCAATCCTACACAT
  GAGAAAGTGTTGGCCATTGTCAATGCCCTGGCGGAGAATCGGGCCATTCGCCCCGATGAGGCCGGCCTGG
  TATACGATGCCCTTTTGCAACGGGTGGCTCGGTATAATTCTGGCAACGTGCAGACTAACCTGGACAGACT
  GGTGGGGGACGTGAGAGAAGCAGTTGCTCAGCGAGAACGGGCTCAGCAACAGGGCAATCTGGGAAGCATG
  GTAGCTCTCAACGCTTTTCTGAGCACCCAGCCTGCAAACGTCCCGCGGGGGCAAGAGGACTACACAAACT
  TTGTCTCCGCCCTGAGACTGATGGTCACCGAAACACCTCAGTCAGAGGTCTATCAGTCCGGTCCCGACTA
  TTTTTTCCAGACATCCAGGCAGGGCCTCCAGACCGTGAATCTGTCTCAGGCATTTAAGAATCTCCAGGGT
  CTGTGGGGTGTTAGGGCGCCGACTGGTGATCGGGCCACGGTCTCCTCTCTGTTGACCCCCAACAGCAGGC
  TGCTGCTTCTGTTGATCGCGCCATTCACTGACTCCGGATCCGTTTCCAGGGATACGTATCTCGGCCACCT
  GCTTACGCTTTACAGGGAAGCTATTGGCCAAGCGCATGTCGACGAACATACATTCCAGGAGATAACCTCT
  GTGTCCAGGGCTCTGGGCCAGGAGGACACAGGGAGTCTGGAAGCCACTTTGAACTATCTGCTTACCAACC
  GGCGGCAGAAAATTCCCTCTCTCCATTCCCTCAACTCAGAAGAGGAAAGGATCCTGAGGTATGTTCAGCA
  GAGCGTTTCTCTGAACCTGATGAGGGATGGCGTGACCCCAAGTGTGGCCCTTGACATGACAGCCAGGAAC
  ATGGAACCTGGAATGTATGCGTCCAACAGACCCTTTATTAATCGCCTGATGGATTATCTGCACCGCGCTG
  CCGCAGTGAACCCCGAATACTTCACTAATGCCATCCTGAACCCACACTGGCTTCCGCCACCCGGATTTTA
  CACTGGCGGATTCGAAGTCCCAGAAGGCAACGACGGCTTCCTGTGGGATGATATCGACGATAGTGTATTT
  TCCCCTCAGCCACAGACACTTCTTGAGCTGCAGCAGAGAGAACAAGCCGAAGCCGCCCTGCGGAAGGAAA
  GTTTTCGAAGGCCATCCAGTCTCTCCGATTTGGGCGCGGCTGCACCTCGGTCCGATGCAAGCTCTCCATT
  TCCTAGCCTCATCGGATCCCTGACATCCACAAGGACCACCAGGCCAAGACTGTTGGGAGAGGAAGAATAC
  CTGAATAACAGCCTGCTTCAGCCACAGCGAGAAAAAAACCTGCCTCCCGCCTTTCCCAACAATGGCATTG
  AGTCTCTCGTGGATAAAATGAGTAGATGGAAGACTTACGCACAGGAGCACCGCGATGTCCCTGGCCCCAG
  GCCGCCGACTCGGAGGCAGAGACATGACAGGCAACGGGGACTGGTATGGGAGGACGATGATTCAGCCGAT
  GACAGCAGCGTCCTGGACCTGGGTGGCTCCGGCAATCCGTTCGCACACCTTCGGCCCCGGTTGGGGCGCA
  TGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100009 representing AP_000506
  Red=Cloning sites Green=Tags
  
  MMQDATDPAVRAALQSQPSGLNSTDDWRQVMDRIMSLTARNPDAFRQQPQANRLSAILEAVVPARANPTH
  EKVLAIVNALAENRAIRPDEAGLVYDALLQRVARYNSGNVQTNLDRLVGDVREAVAQRERAQQQGNLGSM
  VALNAFLSTQPANVPRGQEDYTNFVSALRLMVTETPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLQG
  LWGVRAPTGDRATVSSLLTPNSRLLLLLIAPFTDSGSVSRDTYLGHLLTLYREAIGQAHVDEHTFQEITS
  VSRALGQEDTGSLEATLNYLLTNRRQKIPSLHSLNSEEERILRYVQQSVSLNLMRDGVTPSVALDMTARN
  MEPGMYASNRPFINRLMDYLHRAAAVNPEYFTNAILNPHWLPPPGFYTGGFEVPEGNDGFLWDDIDDSVF
  SPQPQTLLELQQREQAEAALRKESFRRPSSLSDLGAAAPRSDASSPFPSLIGSLTSTRTTRPRLLGEEEY
  LNNSLLQPQREKNLPPAFPNNGIESLVDKMSRWKTYAQEHRDVPGPRPPTRRQRHDRQRGLVWEDDDSAD
  DSSVLDLGGSGNPFAHLRPRLGRMF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000506
• RefSeq ORF: 1755
• MW: 65.3 kDa