E1B 55K (AC_000017) Virus Tagged ORF Clone

CAT#: VC100003

Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 1], codon optimized for human cell expression, AP_000500

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 55K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100003 represents NCBI reference of AP_000500 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGACGCAATCCAAGCGAGCGGGGCGTACCCGCTGGGTTCAGCGGGCATGCTTTTGTAGAGAGCG
  GTGGAGAAACTCAGGAGTCCCCAACCACAGTGGTGTTCAGACCACCCGGCAATAACACGGACGGAGGCGC
  AGCCGCAGCTACAGCTGCTGCCGGGGGTTCCCAAGCTGCGGCAGCTGCAGGAGCTGAGCCTATGGAACCT
  GAGTCACGACCCGGACCGAGTGGCATGAACGTGGTGCAGGTGGCAGAGCTTTTCCCAGAGCTGAGGAGGA
  TCTTGACAATTAACGAGGATGGCCAAGGACTGAAAGGAGTGAAACGAGAGCGCGGTGCCTTTGAAGCAAC
  AGAGGAAGTGCGCAACCTTACATTCAGTCTGATGACCCGCCATCGGCCGGAGTGTGTCACCTTCCAGCAG
  ATCAAGGACAATTGTGCCAACGAACTTGACCTGCTGGCACAGAAATACTCTATAGAACAGCTTACAACCT
  ACTGGCTGCAGCCCGGAGATGACTTCGAGGAGGCTATTCGCGTTTATGCCAAAGTGGCTCTGAGACCAGA
  CTGTAAGTACAAGATCTCCAAGCTGGTAAATATACGCAACTGTTGCTATATTTCTGGGAACGGTGCAGAA
  GTTGAGATCGATACGGAAGACAGGGTGGCATTTAGATGCTCTATGATAAATATGTGGCCCGGTGTCCTGG
  GTATGGACGGCGTGGTGATCATGAATGTACGGTTTACCGGGCCTAATTTTTCAGGAACCGTGTTCCTGGC
  TAATACCAATCTGATTCTCCATGGAGTCTCCTTTTACGGCTTTAACAATACCTGTGTGGAGGCGTGGACC
  GATGTCCGCGTTAGGGGTTGTGCCTTTTACTGCTGTTGGAAAGGCGTCGTATGCCGGCCCAAGAGTCGCG
  CTAGCATTAAAAAATGTCTTTTTGAGCGATGTACACTGGGAATATTGTCTGAGGGTAATTCCAGGGTTCG
  ACACAACGTAGCCTCTGACTGCGGGTGTTTTATGCTTGTTAAGAGTGTGGCCGTGATCAAGCACAATATG
  GTATGTGGCAACTGTGAAGATAGAGCGTCCCAAATGTTGACGTGTTCAGATGGTAACTGCCATTTGCTTA
  AGACCATTCATGTCGCCTCACATAGTCGGAAGGCATGGCCTGTCTTTGAGCACAACATCCTTACCCGGTG
  TTCACTGCACCTGGGGAATAGACGGGGAGTGTTTCTTCCGTACCAATGCAACCTGTCTCACACCAAAATA
  CTGTTGGAGCCAGAGTCCATGTCAAAAGTCAATCTCAACGGTGTGTTTGATATGACCATGAAGATCTGGA
  AGGTTCTCAGATATGATGAGACTCGGACACGATGCCGCCCTTGTGAGTGCGGCGGTAAACACATTAGAAA
  CCAGCCAGTGATGCTGGACGTTACTGAGGAACTGCGCCCAGACCACCTGGTCCTGGCATGTACGAGGGCC
  GAATTCGGATCTTCTGATGAGGACACCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100003 representing AP_000500
  Red=Cloning sites Green=Tags
  
  MERRNPSERGVPAGFSGHAFVESGGETQESPTTVVFRPPGNNTDGGAAAATAAAGGSQAAAAAGAEPMEP
  ESRPGPSGMNVVQVAELFPELRRILTINEDGQGLKGVKRERGAFEATEEVRNLTFSLMTRHRPECVTFQQ
  IKDNCANELDLLAQKYSIEQLTTYWLQPGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAE
  VEIDTEDRVAFRCSMINMWPGVLGMDGVVIMNVRFTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWT
  DVRVRGCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNSRVRHNVASDCGCFMLVKSVAVIKHNM
  VCGNCEDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGNRRGVFLPYQCNLSHTKI
  LLEPESMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGGKHIRNQPVMLDVTEELRPDHLVLACTRA
  EFGSSDEDTD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000500
• RefSeq ORF: 1500
• MW: 55.4 kDa