V (AC_000017) Virus Tagged ORF Clone

CAT#: VC100012

Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 1], codon optimized for human cell expression, AP_000509

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: V
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100012 represents NCBI reference of AP_000509 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAGAGAAAAATCAAGGAGGAGATGCTGCAGGTGATCGCCCCTGAGATCTACGGACCACCCAAAA
  AGGAAGAGCAGGACTATAAACCTCGGAAACTTAAAAGGGTGAAGAAGAAGAAAAAGGATGACGATGACGA
  ACTCGACGATGAGGTGGAACTCCTTCACGCCACTGCGCCCCGCCGGCGGGTGCAGTGGAAGGGGAGGCGG
  GTCAGAAGAGTCCTGAGGCCAGGTACGACCGTCGTGTTTACTCCAGGAGAGAGGTCCACCAGGACTTACA
  AGAGAGTGTACGATGAAGTGTACGGTGATGAGGATCTCCTGGAACAGGCTAATGAACGGCTCGGCGAATT
  CGCATACGGAAAGAGACATAAGGACATGCTCGCCCTGCCACTGGATGAGGGAAATCCTACTCCCTCTCTG
  AAACCTGTGACTTTGCAGCAGGTTCTTCCCGCACTGGCTCCATCTGAAGAGAAACGAGGCCTGAAAAGGG
  AATCTGGGGATCTGGCACCAACAGTTCAACTGATGGTGCCGAAGAGGCAGCGGCTGGAGGACGTGCTTGA
  AAAGATGACTGTCGAACCTGGACTGGAGCCCGAGGTGCGAGTAAGACCTATCAAACAAGTGGCACCAGGA
  CTTGGAGTGCAGACAGTCGATGTTCAAATCCCTACAACCAGCAGTACGAGCATTGCCACTGCTACTGAGG
  GGATGGAAACCCAGACCTCCCCCGTCGCCTCCGCCGTGGCCGACGCAGCGGTTCAGGCTGCAGCGGCGGC
  TGCTTCAAAGACTTCTACTGAAGTGCAGACTGACCCATGGATGTTTAGAGTGAGCGCCCCTCGCAGGCCC
  CGCAGATCACGAAAGTATGGAGCAGCATCAGCCTTGCTCCCTGAGTACGCTTTGCATCCATCTATTGCCC
  CTACACCTGGGTATCGCGGGTACACCTACAGGCCCAGACGGCGGGCCACAACGCGGCGCCGGATCACCAC
  TGGGACTAGAAGGAGGCGACGCCGAAGGCAGCCAGTTCTCGCACCTATCTCTGTCCGGCGAGTGGCCCGG
  GAGGGTGGCAGGACCCTCGTCCTCCCCACGGCACGCTATCACCCCAGCATCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100012 representing AP_000509
  Red=Cloning sites Green=Tags
  
  MSKRKIKEEMLQVIAPEIYGPPKKEEQDYKPRKLKRVKKKKKDDDDELDDEVELLHATAPRRRVQWKGRR
  VRRVLRPGTTVVFTPGERSTRTYKRVYDEVYGDEDLLEQANERLGEFAYGKRHKDMLALPLDEGNPTPSL
  KPVTLQQVLPALAPSEEKRGLKRESGDLAPTVQLMVPKRQRLEDVLEKMTVEPGLEPEVRVRPIKQVAPG
  LGVQTVDVQIPTTSSTSIATATEGMETQTSPVASAVADAAVQAAAAAASKTSTEVQTDPWMFRVSAPRRP
  RRSRKYGAASALLPEYALHPSIAPTPGYRGYTYRPRRRATTRRRITTGTRRRRRRRQPVLAPISVRRVAR
  EGGRTLVLPTARYHPSIV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000509
• RefSeq ORF: 1104
• MW: 41.6 kDa