IX (AC_000017) Virus Tagged ORF Clone

CAT#: VC100004

Myc-DDK-tagged ORF clone of viral ORF for IX [Human adenovirus 1], codon optimized for human cell expression, AP_000501

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100004 represents NCBI reference of AP_000501 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTACAAATAGCTTTGACGGCTCTATCGTTAGCTCCTACCTGACAACACGGATGCCTCCCTGGGCCG
  GAGTCAGACAAAATGTGATGGGGTCCTCAATCGACGGCAGGCCCGTGCTGCCCGCCAACTCTACTACATT
  GACATATGAGACTGTTTCTGGCACCCCCCTTGAGACTGCAGCGAGCGCTGCCGCTTCTGCAGCGGCGGCC
  ACTGCCAGAGGGATCGTCACTGACTTTGCTTTCCTCAGTCCCCTGGCAAGTAGCGCCGCTTCCAGATCCA
  GTGCACGGGACGACAAGCTGACTGCGTTGCTGGCCCAACTGGATTCATTGACCAGAGAGCTGAACGTGGT
  AAGCCAGCAGCTCCTGGAGCTTAGGCAACAGGTGAGTGCACTGAAGGCTTCAAGCCCACCAAATGCTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100004 representing AP_000501
  Red=Cloning sites Green=Tags
  
  MSTNSFDGSIVSSYLTTRMPPWAGVRQNVMGSSIDGRPVLPANSTTLTYETVSGTPLETAASAAASAAAA
  TARGIVTDFAFLSPLASSAASRSSARDDKLTALLAQLDSLTRELNVVSQQLLELRQQVSALKASSPPNAV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 420 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000501
• RefSeq ORF: 420
• MW: 14.5 kDa