pTP (AC_000017) Virus Tagged ORF Clone

CAT#: VC100007

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 1], codon optimized for human cell expression, AP_000504

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pTP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100007 represents NCBI reference of AP_000504 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTTTCAGTGAACGATTGCGCTCGGCTCACGGGGCAGTCCGTTCCTACAATGGAGCACTTCTTGC
  CCCTGCGGAATATTTGGAACCGCGTCCGCGATTTCCCTAGAGCATCTACCACAGCCGCAGGCATCACATG
  GATGAGCAGATACATCTATGGGTACCACAGGCTGATGCTGGAGGACCTCGCTCCAGGCGCGCCAGCCACA
  CTGCGCTGGCCACTGTATCGACAACCACCTCCTCACTTTCTTGTTGGCTATCAATACTTGGTACGGACAT
  GCAACGATTACGTGTTTGACAGCCGGGCCTATTCCCGCCTCAGGTACACAGAGCTGAGCCAGCCTGGACA
  CCAGACCGTGAACTGGAGTGTGATGGCTAATTGTACGTATACCATAAATACTGGCGCTTATCATAGGTTC
  GTTGACATGGACGATTTTCAATCAACTCTGACCCAGGTACAACAGGCTATTCTCGCAGAAAGGGTGGTGG
  CCGATCTGGCACTGCTCCAGCCCATGAGAGGGTTTGGCGTGACCAGAATGGGAGGCAGAGGGCGCCACCT
  GAGGCCCAATAGTGCTGCAGCTGCTGCTATCGACGCCCGGGATGCTGGGCAGGAGGAGGGCGAAGAAGAG
  GTGCCAGTGGAGAGGCTGATGCAAGACTACTACAAGGATCTCCGACGGTGCCAGAACGAGGCATGGGGAA
  TGGCCGATAGACTCCGCATTCAGCAGGCGGGACCCAAGGACATGGTGCTCCTGAGCACCATACGGCGATT
  GAAGACAGCTTACTTCAACTATATTATAAGCTCAACGAGCGCTAGGAACAACCCCGATAGGAGACCGCTG
  CCCCCAGCCACCGTGTTGAGCCTGCCGTGTGACTGTGACTGGCTGGACGCTTTTCTGGAGAGGTTCTCTG
  ACCCAGTGGATGCCGATTCCCTGCGCTCTCTGGGTGGTGGAGTTCCAACGCAGCAGCTGCTGAGATGCAT
  AGTTTCAGCCGTGTCTCTGCCACATGGATCCCCCCCTCCAACCCATAACCGGGACATGACTGGTGGAGTC
  TTCCAACTCCGGCCTCGGGAAAATGGACGGGCAGTCACTGAAACTATGCGGAGAAGACGGGGAGAAATGA
  TTGAAAGGTTTGTCGACAGGCTGCCAGTTAGACGAAGAAGAAGACGAGTCCCACCGCCGCCACCCCCACC
  TGAGGAAGAAGAGGGCGAAGCTCTGATGGAAGAAGAAATCGAGGAGGAAGAAGCCCCCGTGGCATTCGAG
  CGGGAAGTCAGAGACACTGTGGCCGAGCTCATCAGACTGCTGGAGGAAGAACTGACAGTGAGTGCCAGAA
  ACAGCCAGTTCTTTAATTTTGCCGTGGACTTTTACGAGGCAATGGAAAGGCTGGAGGCTTTGGGCGACAT
  TAACGAGTCTACTCTGCGACGGTGGGTGATGTATTTCTTCGTGGCCGAACATACCGCCACCACACTTAAC
  TACCTGTTCCAACGACTCCGCAATTATGCCGTTTTTGCCAGGCACGTTGAGCTGAACTTGGCACAGGTGG
  TGATGAGAGCACGCGATGCCGAAGGCGGAGTGGTTTATTCCAGGGTATGGAATGAGGGGGGCCTGAATGC
  ATTTTCCCAACTGATGGCCCGGATATCCAACGACCTGGCGGCCACAGTTGAACGGGCTGGCCGCGGCGAT
  CTTCAAGAGGAGGAGATCGAGCAATTTATGGCCGAAATCGCGTATCAGGACAATTCTGGCGACGTTCAGG
  AGATCCTCAGGCAGGCCGCCGTCAACGACACGGAAATTGATTCAGTTGAACTGAGTTTCCGCTTCAAGCT
  TACCGGCCCGGTCGTATTCACGCAGCGACGACAGATCCAAGAGATTAACCGGCGGGTTGTGGCATTTGCC
  AGCAATTTGCGCGCACAGCACCAATTGCTGCCTGCTAGAGGCGCAGATGTTCCCCTGCCACCACTGCCAG
  CTGGTCCCGAGCCTCCGCTCCCACCAGGTGCGCGGCCTCGACACCGATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100007 representing AP_000504
  Red=Cloning sites Green=Tags
  
  MALSVNDCARLTGQSVPTMEHFLPLRNIWNRVRDFPRASTTAAGITWMSRYIYGYHRLMLEDLAPGAPAT
  LRWPLYRQPPPHFLVGYQYLVRTCNDYVFDSRAYSRLRYTELSQPGHQTVNWSVMANCTYTINTGAYHRF
  VDMDDFQSTLTQVQQAILAERVVADLALLQPMRGFGVTRMGGRGRHLRPNSAAAAAIDARDAGQEEGEEE
  VPVERLMQDYYKDLRRCQNEAWGMADRLRIQQAGPKDMVLLSTIRRLKTAYFNYIISSTSARNNPDRRPL
  PPATVLSLPCDCDWLDAFLERFSDPVDADSLRSLGGGVPTQQLLRCIVSAVSLPHGSPPPTHNRDMTGGV
  FQLRPRENGRAVTETMRRRRGEMIERFVDRLPVRRRRRRVPPPPPPPEEEEGEALMEEEIEEEEAPVAFE
  REVRDTVAELIRLLEEELTVSARNSQFFNFAVDFYEAMERLEALGDINESTLRRWVMYFFVAEHTATTLN
  YLFQRLRNYAVFARHVELNLAQVVMRARDAEGGVVYSRVWNEGGLNAFSQLMARISNDLAATVERAGRGD
  LQEEEIEQFMAEIAYQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVVFTQRRQIQEINRRVVAFA
  SNLRAQHQLLPARGADVPLPPLPAGPEPPLPPGARPRHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000504
• RefSeq ORF: 2010
• MW: 76.4 kDa