52K (AC_000017) Virus Tagged ORF Clone

CAT#: VC100008

Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 1], codon optimized for human cell expression, AP_000505

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 52K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100008 represents NCBI reference of AP_000505 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCCGTGCTTAGGCAAATGCGGCCCCCCCCGCAACAGAGGCAAGAACAGGAACAGAGACAAACCT
  GCAGGGCTCCGTCACCGTCTCCGACCGCTTCAGGAGGAGCCACAAGCGCCGCAGACGCCGCAGCCGATGG
  AGATTATGAGCCCCCTCGGCGGAGGGCTAGACATTATCTCGACCTGGAAGAGGGCGAAGGCCTCGCTAGA
  CTTGGAGCTCCAAGCCCGGAGCGGCACCCTCGCGTACAACTGAAAAGAGATACTAGGGAAGCCTACGTGC
  CTAGACAAAACTTGTTCCGGGACAGGGAGGGTGAGGAACCCGAGGAAATGCGCGACCGAAAATTCCATGC
  AGGACGAGAGCTGCGGCATGGCCTGAATCGCGAGAGACTCCTCCGGGAGGAGGATTTCGAACCTGACGCT
  AGAACGGGCATCAGCCCAGCCAGAGCCCATGTGGCGGCAGCAGACCTTGTGACCGCCTATGAACAAACAG
  TGAATCAGGAAATCAACTTCCAGAAGTCATTTAATAATCACGTTAGGACTCTGGTGGCCCGCGAGGAGGT
  GGCCATTGGGTTGATGCACCTGTGGGACTTTGTGTCAGCACTGGAACAGAACCCCAACTCCAAACCCCTC
  ATGGCTCAATTGTTCCTCATTGTGCAACATAGCAGAGACAACGAAGCATTTCGAGATGCTCTCCTGAACA
  TCGTAGAACCCGAAGGAAGGTGGCTCCTGGACCTCATTAATATTCTTCAGTCCATAGTCGTGCAGGAGAG
  ATCCCTGTCTCTGGCTGATAAGGTCGCCGCCATCAACTATTCTATGCTGTCTCTCGGTAAATTTTACGCT
  CGAAAGATTTATCACACCCCATACGTGCCGATTGACAAAGAGGTGAAGATCGAGGGATTTTACATGAGAA
  TGGCTTTGAAGGTATTGACCCTTAGCGACGATCTGGGCGTATACAGAAACGAGAGGATCCATAAGGCCGT
  GTCAGTGTCAAGACGCCGCGAATTGTCTGACAGAGAGCTCATGCATTCCCTTCAGCGCGCACTCGCCGGC
  ACTGGCTCCGGAGATAGGGAAGCGGAATCATACTTCGATGCCGGTGCTGACCTTCGGTGGGCTCCCTCAA
  GGAGAGCTCTGGAAGCTGCCGGTGCAGGACCCGGATTGGCTGTCGCTCCTGCAAGGGCCGGCAACGTTGG
  TGGGGTTGAAGAGTACGACGAAGATGACGAGTATGAACCCGAGGATGGCGAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100008 representing AP_000505
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPPPQQRQEQEQRQTCRAPSPSPTASGGATSAADAAADGDYEPPRRRARHYLDLEEGEGLAR
  LGAPSPERHPRVQLKRDTREAYVPRQNLFRDREGEEPEEMRDRKFHAGRELRHGLNRERLLREEDFEPDA
  RTGISPARAHVAAADLVTAYEQTVNQEINFQKSFNNHVRTLVAREEVAIGLMHLWDFVSALEQNPNSKPL
  MAQLFLIVQHSRDNEAFRDALLNIVEPEGRWLLDLINILQSIVVQERSLSLADKVAAINYSMLSLGKFYA
  RKIYHTPYVPIDKEVKIEGFYMRMALKVLTLSDDLGVYRNERIHKAVSVSRRRELSDRELMHSLQRALAG
  TGSGDREAESYFDAGADLRWAPSRRALEAAGAGPGLAVAPARAGNVGGVEEYDEDDEYEPEDGEY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000505
• RefSeq ORF: 1245
• MW: 47.0 kDa