DBP (AC_000017) Virus Tagged ORF Clone

CAT#: VC100017

Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 1], codon optimized for human cell expression, AP_000514

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100017 represents NCBI reference of AP_000514 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCAGAGAGGAGGAGCAGCGAGAAACGACTCCAGAGAGGGGAAGGGGGGCCGCCCGACGACCCC
  CCACCATGGAGGATGTGTCTTCCCCTTCTCCTTCACCGCCACCACCGAGAGCGCCACCCAAGAAAAGGCT
  GCGGAGGAGACTGGAGAGTGAAGACGAGGAGGACAGCAGTCAGGACGCTCTTGTGCCCAGGACTCCTTCA
  CCCCGCCCCAGCACGTCAGCCGCCGATCTCGCCAACGCACCAAAGAAGAAGAAGAAACGGCCATCACCAA
  AACCTGAACGGCCTCCAAGCCCGGAAGTAATTGTTGACTCTGAGGAAGAACGGGAGGATGTGGCTCTCCA
  AATGGTAGGCTTCAGCAATCCCCCTGTATTGATCAAACACGGTAAGGGGGGCAAACGGACAGTGAGACGG
  CTGAACGAGGATGACCCAGTCGCACGAGGCATGAGAATGCAAGAAGAGGAAGAGGAGCCCTCTGAGGCTG
  AATCCGAGTCAACAGTAATGAATCCTCTGTCCCTGCCGATCGTGTCTGCGTGGGAGAAGGGAATGGAAGC
  CGCCAGAGCTCTCATGGATAAGTACCACGTAGACAACGATCTCAAGGCTAATTTTAAGCTCCTGCCAGAC
  CAGGTTGAAGCCCTGGCCGCCGTGTGCAAAACCTGGCTGAACGAAGAGCATAGAGGACTGCAGTTGACAT
  TTACGAGCAACAAGACCTTCGTTACCATGATGGGGAGATTCCTCCAGGCTTACCTGCAGTCCTTCGCAGA
  AGTGACATATAAGCATCACGAGCCTACTGGCTGTGCATTGTGGCTGCACAGGTGCGCAGAGATTGAGGGC
  GAACTTAAATGCCTGCATGGTAGCATTATGATCAATAAAGAACACGTGATCGAGATGGACGTGACCAGTG
  AGAACGGGCAACGCGCCCTGAAGGAGCAGTCTAGCAAGGCCAAGATCGTAAAAAACAGGTGGGGCAGATC
  CGTTGTACAGATTAGTAATACCGACGCACGCTGTTGCGTGCACGACGCCGCCTGCCCAGCAAACCAGTTC
  AGCGGCAAATCCTGTGGCATGTTCTTTTCCGAAGGCGCCAAGGCCCAAGTTGCCTTCAAGCAGATCCAGG
  CATTCATGAAAGCACTTTATCCAAATGCGCAGACTGGACACGGTCACCTTCTTATGCCCCTGCGATGCGA
  GTGCAATTCTAAGCCGGGACACGCCCCATTTCTGGGGCGGCAGCTGCCAAAACTCACTCCCTTCGCCCTG
  TCTAATGCCGAGGATCTTGACGCCGACCTGATCAGCGACAAGAGCGTGCTTGCGTCCGTGCATCATCCCG
  CCCTGATCGTGTTTCAGTGCTGTAACCCAGTGTATCGAAATAGCCGCGCCCAGGGTGGCGGCCCTAACTG
  TGACTTTAAGATTAGCGGCCCAGACCTCTTGAACGCCTTGGTCATGGTTAGATCATTGTGGTCCGAGAAC
  TTTACAGAGCTTCCACGCATGGTTGTACCCGAGTTCAAATGGAGTACCAAGCACCAATACAGGAATGTAA
  GCCTGCCCGTCGCTCACAGTGACGCCAGGCAGAACCCATTCGACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100017 representing AP_000514
  Red=Cloning sites Green=Tags
  
  MASREEEQRETTPERGRGAARRPPTMEDVSSPSPSPPPPRAPPKKRLRRRLESEDEEDSSQDALVPRTPS
  PRPSTSAADLANAPKKKKKRPSPKPERPPSPEVIVDSEEEREDVALQMVGFSNPPVLIKHGKGGKRTVRR
  LNEDDPVARGMRMQEEEEEPSEAESESTVMNPLSLPIVSAWEKGMEAARALMDKYHVDNDLKANFKLLPD
  QVEALAAVCKTWLNEEHRGLQLTFTSNKTFVTMMGRFLQAYLQSFAEVTYKHHEPTGCALWLHRCAEIEG
  ELKCLHGSIMINKEHVIEMDVTSENGQRALKEQSSKAKIVKNRWGRSVVQISNTDARCCVHDAACPANQF
  SGKSCGMFFSEGAKAQVAFKQIQAFMKALYPNAQTGHGHLLMPLRCECNSKPGHAPFLGRQLPKLTPFAL
  SNAEDLDADLISDKSVLASVHHPALIVFQCCNPVYRNSRAQGGGPNCDFKISGPDLLNALVMVRSLWSEN
  FTELPRMVVPEFKWSTKHQYRNVSLPVAHSDARQNPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000514
• RefSeq ORF: 1587
• MW: 59.1 kDa