fiber (AC_000017) Virus Tagged ORF Clone

CAT#: VC100030

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 1], codon optimized for human cell expression, AP_000527

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: fiber
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100030 represents NCBI reference of AP_000527 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCGCGAGACCGAGCGAGGACACATTTAATCCCGTTTACCCATATGATACCGAGACGGGGCCAC
  CAACAGTCCCATTTCTGACACCTCCATTTGTCAGTCCAAACGGATTCCAGGAGTCCCCTCCTGGCGTATT
  GTCCCTGCGGCTGAGCGAACCACTGGTTACATCACATGGAATGTTGGCATTGAAAATGGGGTCTGGCCTG
  GCACTCGATAAAGCAGGCAACTTGACGAGTCAGAACATTACCACTGTGACAGAACCTCTGAAGAAAACTA
  AGTCAAACATTTCACTCGAGACCAGCGCCCCACTGACCGTAACCAGCGGCATTCTCACAGTTGCAACCGC
  TGCACCCCTGGTTGTGGCAGGTAATAGTCTGACAGTTCAGTCACAGGCTCCCCTGACAGTGCAGGACAGT
  AAGCTCAGCATTGCGACCAAGGGTCCACTGACCGTCAGCGAGGGAAAACTCGCACTGCAGACATCAGGAC
  CCCTCTCCGCTACAGATAATAATACACTTACGATTACGACCAGTCCACCCATCACAACTACAAACGGTTC
  CCTGGGCGTGAATATGGAGAATCCTCTGTACAATAATAATGGTAAGTTGGGCCTCCGGGTGGCGGGTCCT
  CTGCAGGTGACTAATGATAGTCACGCATTGACAGTCGGTACTGGGCAGGGGGTCGCCATCGACAACAACG
  CTTTGCATACCAAAGTTACAGGGGCTATTGGCTACGACACCAGCGGCAATATGGAGCTCAAGACCGGTGG
  CGGAGTCCGCGTGGATTCTGTGAACCGACGGTTGATACTGGATGTAGACTATCCATTTGATGCCCAGTCC
  CAGCTGCGCCTGAAACTCGGCCAGGGGCCACTGTATGTGAATTCTAGTACTCACAATCTCGACTTGAATT
  ACAACAAAGGCCTTCACCTTTTCACGACGGGCAACTCCAAGAAGCTGGAGGTGAACCTTAAAACTACGAA
  AGGACTGATTTTTGATACCGATGCCGTGGCAATTCACGCTGCCCAGGGCCTGGAATTTGGCAACGATACA
  TCAACTAACACCAACCCCCTGAAAACTAAACTCGGGCTCGGTCTCGACTACGACAGCAACGGCGGCATGA
  TACCCAAGCTTGGGACCGGCCTGAGTTTCGACACAACAGGAGCTATCACAGTTGGCAACAAGAGCGACGA
  TAAACTGACTCTTTGGACCACCCCGGATCCCAGCCCTAATTGTCAAATACACTCTGAGAAAGACGCTAAG
  CTCACTTTGGTACTGACCAAATGCGGCAGTCAGGTGCTGGCTACCGTAAGTGCTCTGGCAGTGCGAGGCA
  GCCTGGCTCCAATCTCCGGAACAATTTCCTCAGCCCATATCATACTGCGGTTCAACGAACATGGTGTGCT
  GATGAACCACTCTAGCCTCGACCCCCAATACTGGAATTTCCGCAAGGGTGATCTGACAAACGCCACAGCC
  TATACGAACGCAGTGGGATTCATGCCAAATCTTAAAGCCTATCCCAAGACCCAGAGCCGCACGGCTAAAT
  CTAACATAGTTAGCCAAGTATATCTGAACGGAGAGAAGGAAAAACCAATGACTCTTACCATTACCCTCAA
  CGGGACAGACGAAAATCAAACTACCCCTGCCTCTACCTACTCTATTTCATTTTCTTGGTCATGGCCAAGT
  AATCAAACCTATATTGGGCAGACATTCGCGACTAACTCTTATACTTTCAGTTATATTGCTCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100030 representing AP_000527
  Red=Cloning sites Green=Tags
  
  MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPGVLSLRLSEPLVTSHGMLALKMGSGL
  ALDKAGNLTSQNITTVTEPLKKTKSNISLETSAPLTVTSGILTVATAAPLVVAGNSLTVQSQAPLTVQDS
  KLSIATKGPLTVSEGKLALQTSGPLSATDNNTLTITTSPPITTTNGSLGVNMENPLYNNNGKLGLRVAGP
  LQVTNDSHALTVGTGQGVAIDNNALHTKVTGAIGYDTSGNMELKTGGGVRVDSVNRRLILDVDYPFDAQS
  QLRLKLGQGPLYVNSSTHNLDLNYNKGLHLFTTGNSKKLEVNLKTTKGLIFDTDAVAIHAAQGLEFGNDT
  STNTNPLKTKLGLGLDYDSNGGMIPKLGTGLSFDTTGAITVGNKSDDKLTLWTTPDPSPNCQIHSEKDAK
  LTLVLTKCGSQVLATVSALAVRGSLAPISGTISSAHIILRFNEHGVLMNHSSLDPQYWNFRKGDLTNATA
  YTNAVGFMPNLKAYPKTQSRTAKSNIVSQVYLNGEKEKPMTLTITLNGTDENQTTPASTYSISFSWSWPS
  NQTYIGQTFATNSYTFSYIAQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 1746 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000527
• RefSeq ORF: 1746
• MW: 61.8 kDa