33K (AC_000017) Virus Tagged ORF Clone

CAT#: VC100019

Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 1], codon optimized for human cell expression, AP_000516

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 33K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100019 represents NCBI reference of AP_000516 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCTAAAAAGAAGCTGCAACTCCCCCCTCCCCCTACCGATGAAGAGGAATACTGGGATTCCCAAG
  CGGAGGAGGTGCTGGACGAAGAGGAGGAAGACATGATGGAAGATTGGGAGAGCTTGGACGAGGAAGCCTC
  TGAGGCTGAGGAAGTCAGCGACGAAACTCCTTCACCCTCCGTGGCGTTCCCCTCTCCAGCCCCACAGAAG
  AGTGCGACCGGTCCCTCCATGGCAACTACAAGTGCACCCCAAGCCCCTCCAGCCCTGCCCGTTAGACGGC
  CTAACCGACGGTGGGACACAACAGGTACAAGGGCTGCACCAACTGCCCCAGCCGCAGCTGCTGCAACCGC
  TGCCGCCACCCAGAAACAACGCAGGCCAGACTCCAAAACCCTCACCAAGCCCAAGAAGTCTACCGCTGCA
  GCTGCTGCCGGCGGCGGGGCTCTCCGCCTTGCTCCCAACGAGCCAGTCAGTACACGGGAGTTGCGCAACC
  GGATTTTTCCCACACTGTACGCTATCTTCCAACAGTCCAGGGGGCAAGAGCAAGAGCTTAAGATAAAGAA
  TCGCTCCCTGAGATCTCTGACCCGGAGTTGCCTTTATCATAAAAGCGAAGACCAGTTGCGGAGAACTCTC
  GAAGACGCAGAGGCTCTGTTTTCCAAGTACTGCGCCCTCACTCTGAAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100019 representing AP_000516
  Red=Cloning sites Green=Tags
  
  MAPKKKLQLPPPPTDEEEYWDSQAEEVLDEEEEDMMEDWESLDEEASEAEEVSDETPSPSVAFPSPAPQK
  SATGPSMATTSAPQAPPALPVRRPNRRWDTTGTRAAPTAPAAAAATAAATQKQRRPDSKTLTKPKKSTAA
  AAAGGGALRLAPNEPVSTRELRNRIFPTLYAIFQQSRGQEQELKIKNRSLRSLTRSCLYHKSEDQLRRTL
  EDAEALFSKYCALTLKD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000017
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000017.1, AP_000516
• RefSeq ORF: 681
• MW: 25.0 kDa