E1A (NC_001405) Virus Tagged ORF Clone

CAT#: VC100368

Myc-DDK-tagged ORF clone of viral ORF for control protein E1A 243R [Human adenovirus C], codon optimized for human cell expression, NP_040508

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100368 represents NCBI reference of NP_040508 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACACATAATATGTCATGGTGGAGTGATTACAGAGGAGATGGCAGCTAGCCTGCTCGACCAGCTGA
  TAGAGGAGGTGCTGGCGGATAATCTGCCCCCACCCAGTCATTTCGAGCCCCCTACTCTCCACGAACTGTA
  TGACCTTGACGTCACAGCACCCGAAGATCCGAATGAGGAGGCTGTGTCCCAGATTTTCCCCGAGTCAGTC
  ATGCTGGCCGTGCAGGAAGGGATTGACCTCTTCACATTCCCACCTGCCCCTGGCAGCCCAGAACCCCCCC
  ATCTGTCACGCCAGCCAGAGCAGCCCGAGCAAAGAGCCCTCGGACCTGTGTCTATGCCGAACCTGGTTCC
  CGAAGTAATCGATCTGACATGTCATGAGGCCGGCTTCCCACCTTCCGACGATGAGGACGAAGAAGGCCCA
  GTCTCAGAGCCTGAACCGGAACCCGAGCCTGAGCCTGAGCCCGCGCGCCCTACAAGGCGCCCTAAACTCG
  TGCCGGCAATTTTGAGAAGACCAACCTCCCCTGTTAGCCGCGAGTGCAATTCATCAACTGACTCATGCGA
  CAGTGGCCCATCCAACACCCCTCCCGAAATCCACCCCGTGGTGCCTCTGTGCCCCATTAAGCCTGTGGCA
  GTGCGCGTTGGTGGACGGAGACAGGCCGTGGAGTGCATCGAGGATCTGTTGAACGAGAGCGGCCAGCCCC
  TCGATCTGAGCTGCAAGAGACCAAGACCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100368 representing NP_040508
  Red=Cloning sites Green=Tags
  
  MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHELYDLDVTAPEDPNEEAVSQIFPESV
  MLAVQEGIDLFTFPPAPGSPEPPHLSRQPEQPEQRALGPVSMPNLVPEVIDLTCHEAGFPPSDDEDEEGP
  VSEPEPEPEPEPEPARPTRRPKLVPAILRRPTSPVSRECNSSTDSCDSGPSNTPPEIHPVVPLCPIKPVA
  VRVGGRRQAVECIEDLLNESGQPLDLSCKRPRP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040508
• RefSeq ORF: 729
• Locus ID: 2652980
• MW: 26.5 kDa