E2B (NC_001405) Virus Tagged ORF Clone

CAT#: VC100375

Myc-DDK-tagged ORF clone of viral ORF for terminal protein precursor pTP [Human adenovirus C], codon optimized for human cell expression, NP_040518

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100375 represents NCBI reference of NP_040518 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCTCCGTGAATGATTGCGCCAGACTTACTGGACAGAGCGTACCTACAATGGAACACTTCCTCC
  CGTTGCGGAACATATGGAACCGAGTAAGAGATTTCCCACGAGCGTCAACAACTGCAGCAGGGATCACTTG
  GATGTCAAGGTACATCTATGGGTACCATCGCCTTATGTTGGAAGACCTCGCACCTGGAGCACCCGCTACG
  CTCCGCTGGCCTTTGTATCGACAGCCACCTCCTCATTTCCTGGTGGGGTATCAGTATTTGGTTAGGACAT
  GTAACGATTACGTCTTTGACTCAAGAGCTTATTCACGGCTTCGATATACAGAACTCAGTCAGCCCGGCCA
  TCAGACCGTGAACTGGAGCGTTATGGCAAACTGTACGTATACCATCAATACAGGGGCCTACCATCGCTTC
  GTCGACATGGACGATTTTCAGTCAACCCTTACGCAGGTGCAACAGGCCATTCTTGCTGAGAGAGTAGTGG
  CCGACTTGGCCCTGCTGCAACCTATGAGGGGCTTCGGTGTGACCCGGATGGGAGGTCGGGGTCGGCACCT
  GCGCCCTAACTCCGCTGCTGCTGTTGCTATTGACGCTAGAGATGCCGGTCAGGAGGAGGGCGAGGAGGAA
  GTCCCTGTGGAGCGACTGATGCAGGACTACTATAAAGATCTGAGGCGGTGTCAGAATGAAGCTTGGGGAA
  TGGCTGATCGCCTCCGGATTCAGCAGGCTGGACCTAAAGATATGGTGCTGCTGTCCACTATCAGGCGACT
  CAAGACCGCCTATTTTAACTACATCATCTCCTCCACCAGCGCCCGGAATAATCCCGATAGACACCCGCTC
  CCACCAGCCACTGTATTGTCCCTGCCCTGCGATTGCGACTGGCTGGATGCCTTTCTCGAGCGGTTCTCTG
  ACCCCGTCGATGCAGACTCATTGAGATCCCTGGGTGGCGGAGTTCCTACCCAGCAGCTTCTGAGGTGTAT
  TGTGTCAGCCGTGTCTCTCCCCCACGGTAGTCCCCCCCCTACCCACAATAGAGACATGACAGGTGGAGTC
  TTCCAGCTCAGACCACGGGAGAACGGGCGAGCAGTGACAGAAACAATGAGACGAAGAAGGGGGGAGATGA
  TCGAACGGTTCGTTGATAGGCTGCCTGTGAGGAGGAGGAGGCGACGGGTCCCACCGCCTCCACCACCACC
  TGAAGAGGAGGAAGAAGGTGAGGCTCTGATGGAGGAGGAGATCGAGGAGGAGGAGGCCCCGGTTGCTTTT
  GAACGAGAAGTGAGAGATACCGTAGCCGAGCTGATCCGCCTCCTGGAAGAAGAGTTGACAGTGTCAGCTC
  GCAATTCTCAGTTCTTTAATTTTGCGGTGGACTTCTACGAGGCCATGGAGCGACTTGAGGCCTTGGGAGA
  CATAAACGAGAGCACCCTGAGGAGGTGGGTGATGTACTTTTTCGTGGCTGAGCACACAGCAACAACCCTC
  AATTACCTGTTCCAGAGGTTGAGGAACTACGCCGTGTTTGCTAGACATGTGGAGCTCAACCTCGCACAGG
  TGGTCATGCGAGCAAGGGACGCAGAGGGTGGTGTGGTTTATAGCAGAGTGTGGAATGAAGGCGGTTTGAA
  CGCTTTCTCCCAGCTCATGGCACGAATCTCAAATGACCTGGCAGCCACAGTCGAGCGAGCTGGCCGAGGA
  GATCTTCAGGAGGAGGAGATAGAGCAGTTTATGGCCGAAATCGCATACCAGGATAACAGCGGGGATGTCC
  AGGAGATTCTGAGGCAGGCAGCCGTGAATGATACCGAAATCGACTCTGTAGAACTCTCATTCAGATTTAA
  GCTGACTGGCCCAGTCGTGTTCACCCAGCGCCGGCAGATACAAGAGATTAATCGCCGCGTAGTGGCTTTC
  GCCAGTAATCTGAGAGCGCAGCACCAACTTCTTCCAGCTCGGGGAGCGGACGTACCATTGCCACCCTTGC
  CCGCTGGGCCGGAACCACCACTGCCTCCAGGAGCCAGGCCCAGGCATCGGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100375 representing NP_040518
  Red=Cloning sites Green=Tags
  
  MALSVNDCARLTGQSVPTMEHFLPLRNIWNRVRDFPRASTTAAGITWMSRYIYGYHRLMLEDLAPGAPAT
  LRWPLYRQPPPHFLVGYQYLVRTCNDYVFDSRAYSRLRYTELSQPGHQTVNWSVMANCTYTINTGAYHRF
  VDMDDFQSTLTQVQQAILAERVVADLALLQPMRGFGVTRMGGRGRHLRPNSAAAVAIDARDAGQEEGEEE
  VPVERLMQDYYKDLRRCQNEAWGMADRLRIQQAGPKDMVLLSTIRRLKTAYFNYIISSTSARNNPDRHPL
  PPATVLSLPCDCDWLDAFLERFSDPVDADSLRSLGGGVPTQQLLRCIVSAVSLPHGSPPPTHNRDMTGGV
  FQLRPRENGRAVTETMRRRRGEMIERFVDRLPVRRRRRRVPPPPPPPEEEEEGEALMEEEIEEEEAPVAF
  EREVRDTVAELIRLLEEELTVSARNSQFFNFAVDFYEAMERLEALGDINESTLRRWVMYFFVAEHTATTL
  NYLFQRLRNYAVFARHVELNLAQVVMRARDAEGGVVYSRVWNEGGLNAFSQLMARISNDLAATVERAGRG
  DLQEEEIEQFMAEIAYQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVVFTQRRQIQEINRRVVAF
  ASNLRAQHQLLPARGADVPLPPLPAGPEPPLPPGARPRHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040518
• RefSeq ORF: 2013
• Locus ID: 2652985
• MW: 76.5 kDa