E3B (NC_001405) Virus Tagged ORF Clone

CAT#: VC100395

Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-beta [Human adenovirus C], codon optimized for human cell expression, YP_001551771

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100395 represents NCBI reference of YP_001551771 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCAGTGTCATCTTCGTCCTTCTGATATTTTGCGCCCTGCCTGTGCTGTGCAGCCAGACAAGCG
  CCCCCCCGAAAAGGCATATTTCTTGTCGGTTCACACAAATCTGGAATATTCCGTCCTGTTACAATAAGCA
  GTCCGACCTGTCCGAGGCTTGGCTGTACGCCATAATTAGCGTAATGGTCTTCTGCAGTACGATTTTCGCT
  CTTGCAATCTACCCCTACCTCGACATCGGCTGGAACGCCATTGACGCAATGAACCATCCAACCTTTCCAG
  TCCCAGCAGTCATTCCTTTGCAGCAAGTGATAGCCCCAATTAATCAGCCTCGACCACCGTCACCAACTCC
  AACAGAGATCAGTTACTTCAATCTGACCGGCGGCGACGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100395 representing YP_001551771
  Red=Cloning sites Green=Tags
  
  MKRSVIFVLLIFCALPVLCSQTSAPPKRHISCRFTQIWNIPSCYNKQSDLSEAWLYAIISVMVFCSTIFA
  LAIYPYLDIGWNAIDAMNHPTFPVPAVIPLQQVIAPINQPRPPSPTPTEISYFNLTGGDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, YP_001551771
• RefSeq ORF: 390
• Locus ID: 2652988
• MW: 14.5 kDa