L2 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100380

Myc-DDK-tagged ORF clone of viral ORF for core protein V [Human adenovirus C], codon optimized for human cell expression, NP_040523

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100380 represents NCBI reference of NP_040523 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAGCGCAAAATTAAGGAAGAGATGCTCCAGGTTATAGCACCTGAGATTTACGGACCACCCAAAA
  AAGAGGAACAGGATTATAAGCCTAGAAAGTTGAAGCGGGTGAAGAAAAAGAAAAAAGATGACGATGACGA
  TGAACTCGACGATGAAGTGGAACTCCTGCACGCCACCGCCCCGCGCAGGCGCGTTCAGTGGAAAGGCCGC
  CGAGTGCGAAGGGTGCTGAGGCCAGGAACTACAGTCGTATTTACACCCGGCGAGCGGAGTACCAGAACTT
  ATAAAAGAGTGTATGATGAGGTGTACGGAGATGAGGACCTGCTGGAACAGGCGAACGAGAGGCTGGGCGA
  GTTTGCATACGGGAAACGCCATAAGGATATGCTGGCGCTTCCTCTGGATGAAGGCAACCCTACCCCTTCC
  CTCAAGCCAGTGACCCTGCAGCAGGTCCTGCCCACACTTGCCCCGTCAGAGGAAAAAAGAGGACTGAAAC
  GCGAGTCCGGGGATTTGGCCCCTACAGTTCAACTGATGGTGCCTAAAAGGCAACGCCTCGAAGACGTTCT
  CGAGAAGATGACCGTGGAGCCCGGACTCGAGCCAGAGGTGAGGGTCCGCCCCATTAAACAAGTGGCACCC
  GGACTCGGCGTCCAGACCGTGGATGTACAAATCCCCACCACCAGCAGCACTTCCATTGCCACAGCAACAG
  AGGGAATGGAGACACAAACCTCACCTGTCGCCAGCGCAGTGGCTGATGCTGCTGTTCAGGCCGCAGCTGC
  TGCAGCCTCAAAGACCAGCACCGAAGTCCAGACTGACCCCTGGATGTTTCGGGTATCTGCTCCAAGGCGG
  CCACGGAGATCTCGGAAATACGGCACCGCGTCCGCTCTGCTTCCCGAGTATGCCCTCCACCCAAGTATTG
  CACCTACTCCTGGATATCGGGGATACACTTACAGGCCCCGACGGCGCGCTACTACGAGACGCAGAACAAC
  CACTGGGACCCGACGCAGAAGGAGGAGACGCCAGCCGGTCCTGGCCCCAATCAGCGTTCGGAGGGTGGCC
  CGGGAAGGGGGAAGGACACTTGTGCTGCCCACAGCTAGGTATCACCCAAGCATCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100380 representing NP_040523
  Red=Cloning sites Green=Tags
  
  MSKRKIKEEMLQVIAPEIYGPPKKEEQDYKPRKLKRVKKKKKDDDDDELDDEVELLHATAPRRRVQWKGR
  RVRRVLRPGTTVVFTPGERSTRTYKRVYDEVYGDEDLLEQANERLGEFAYGKRHKDMLALPLDEGNPTPS
  LKPVTLQQVLPTLAPSEEKRGLKRESGDLAPTVQLMVPKRQRLEDVLEKMTVEPGLEPEVRVRPIKQVAP
  GLGVQTVDVQIPTTSSTSIATATEGMETQTSPVASAVADAAVQAAAAAASKTSTEVQTDPWMFRVSAPRR
  PRRSRKYGTASALLPEYALHPSIAPTPGYRGYTYRPRRRATTRRRTTTGTRRRRRRRQPVLAPISVRRVA
  REGGRTLVLPTARYHPSIV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 1107 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040523
• RefSeq ORF: 1107
• Locus ID: 2652996
• MW: 41.7 kDa