E1B (NC_001405) Virus Tagged ORF Clone

CAT#: VC100369

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 19K [Human adenovirus C], codon optimized for human cell expression, NP_040510

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100369 represents NCBI reference of NP_040510 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCATGGGAATGTTTGGAGGACTTCAGCGCCGTGCGAAATCTCCTGGAGCAGTCATCCAACTCCA
  CTTCCTGGTTTTGGAGGTTCCTGTGGGGGAGTAGCCAGGCTAAGCTGGTATGCAGAATAAAAGAAGATTA
  TAAATGGGAGTTTGAGGAGCTGCTCAAGTCTTGCGGCGAGCTCTTCGATTCCTTGAACCTCGGTCACCAA
  GCCCTGTTTCAGGAGAAAGTGATCAAGACTCTCGACTTCAGTACACCTGGACGGGCTGCCGCTGCAGTTG
  CCTTTCTCTCTTTCATCAAAGATAAATGGAGTGAAGAGACCCACCTGTCTGGCGGCTACCTGCTCGATTT
  CCTGGCCATGCATCTGTGGCGAGCCGTTGTCCGGCATAAAAACAGACTGCTTCTCTTGTCTTCAGTGAGA
  CCCGCAATCATACCCACCGAAGAACAGCAGCAGGAAGAAGCTCGGCGCCGCCGCCGGCAAGAGCAAAGCC
  CATGGAACCCGCGCGCAGGCCTCGATCCACGCGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100369 representing NP_040510
  Red=Cloning sites Green=Tags
  
  MEAWECLEDFSAVRNLLEQSSNSTSWFWRFLWGSSQAKLVCRIKEDYKWEFEELLKSCGELFDSLNLGHQ
  ALFQEKVIKTLDFSTPGRAAAAVAFLSFIKDKWSEETHLSGGYLLDFLAMHLWRAVVRHKNRLLLLSSVR
  PAIIPTEEQQQEEARRRRRQEQSPWNPRAGLDPRE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 525 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040510
• RefSeq ORF: 525
• Locus ID: 2652981
• MW: 20.5 kDa