L4 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100386

Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus C], codon optimized for human cell expression, NP_040528

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100386 represents NCBI reference of NP_040528 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGCGTGGAAAAAGAGGATAGCCTTACGGCCCCCTTCGAGTTCGCAACAACCGCATCCACCGACG
  CAGCAAACGCGCCCACTACCTTTCCTGTGGAAGCTCCCCCCTTGGAGGAGGAGGAGGTTATCATCGAGCA
  GGACCCTGGATTTGTGAGCGAAGACGATGAGGATCGCTCAGTTCCAACCGAGGACAAAAAGCAAGATCAG
  GACGACGCTGAGGCCAACGAGGAGCAGGTGGGGAGAGGGGACCAACGCCACGGGGACTATCTTGATGTCG
  GGGACGATGTGCTGCTCAAACACCTGCAACGCCAGTGTGCCATCATTTGTGATGCCCTTCAGGAGAGGAG
  TGACGTTCCCCTCGCTATCGCCGATGTGAGCCTGGCATATGAACGCCATTTGTTCAGCCCTCGAGTCCCC
  CCTAAGAGGCAGGAGAATGGAACTTGTGAACCTAACCCCAGACTTAACTTCTACCCAGTGTTTGCCGTGC
  CCGAAGTCCTCGCTACCTACCACATCTTTTTCCAGAATTGTAAGATCCCCCTGTCATGTCGCGCGAACCG
  GAGCCGGGCAGATAAACAGCTTGCACTTCGCCAGGGCGCGGTGATTCCCGATATTGCCAGCCTGGATGAG
  GTACCAAAGATTTTTGAGGGACTGGGACGCGACGAAAAGCGAGCCGCCAATGCACTGCAGCAGGAGAATA
  GCGAAAATGAAAGTCACTGCGGCGTCCTCGTTGAGCTGGAGGGTGATAACGCCCGCCTGGCGGTGCTCAA
  GAGAAGCATAGAAGTAACCCATTTCGCCTACCCAGCCCTCAATCTGCCACCTAAAGTTATGAGTACCGTC
  ATGTCAGAGTTGATTGTGAGAAGGGCAAGACCTCTGGAGAGGGACGCTAACCTCCAGGAACAAACCGAAG
  AGGGACTCCCAGCCGTGGGCGACGAACAGCTTGCCCGCTGGCTCGAAACTCGGGAGCCGGCTGATCTCGA
  AGAGCGACGGAAACTGATGATGGCCGCGGTGCTGGTGACTGTCGAACTCGAGTGCATGCAGAGGTTCTTT
  GCAGACCCGGAAATGCAGAGAAAGCTGGAGGAGACACTGCACTATACATTCCGCCAGGGATACGTACGGC
  AGGCATGTAAAATCTCCAACGTTGAGCTCTGCAACCTGGTATCCTACCTGGGGATTCTCCACGAGAATAG
  GCTGGGACAGAATGTGCTTCACAGTACGTTGAAAGGAGAGGCAAGACGGGATTATGTGAGAGACTGTGTG
  TATTTGTTCCTGTGCTACACTTGGCAGACTGCGATGGGTGTGTGGCAGCAGTGTCTCGAGGAGCGCAACC
  TGAAAGAACTGCAGAAGCTGCTCAAACAGAACCTGAAAGATCTGTGGACTGCATTCAATGAGCGCAGTGT
  TGCAGCACACCTTGCCGATATTATCTTTCCGGAAAGGCTGCTCAAGACCCTGCAGCAGGGCCTGCCCGAC
  TTCACCTCTCAGTCAATGCTGCAGAACTTCCGAAATTTTATACTGGAGCGGAGCGGCATCCTGCCTGCGA
  CCTGCTGTGCTCTCCCCAGCGACTTCGTGCCGATCAAATATCGCGAGTGTCCTCCCCCTCTGTGGGGACA
  CTGCTATCTGCTGCAGCTGGCTAACTATCTTGCCTATCACTCAGACATCATGGAGGACGTCAGCGGAGAC
  GGACTGCTGGAGTGTCATTGCAGGTGCAACCTCTGTACACCCCACAGGAGTCTCGTATGCAACAGCCAGC
  TGTTGAGCGAGTCCCAGATCATTGGTACCTTTGAGCTGCAAGGACCTTCCCCGGACGAAAAAAGCGCCGC
  CCCCGGGCTCAAACTGACCCCCGGCCTGTGGACTTCCGCATATCTGCGGAAGTTTGTCCCAGAGGACTAT
  CACGCTCATGAAATCCGCTTCTATGAAGACCAGTCCAGACCTCCTAACGCGGAGCTGACCGCATGCGTGA
  TTACCCAGGGACATATCCTGGGCCAGCTGCAGGCTATCAATAAGGCGCGGCAGGAGTTTCTCCTGAGGAA
  GGGCCGCGGAGTGTATCTTGACCCTCAGTCCGGAGAAGAGTTGAACCCTATTCCACCCCCCCCCCAGCCA
  TACCAACAGCCAAGAGCACTCGCCTCCCAGGACGGAACCCAGAAGGAGGCAGCAGCCGCAGCAGCAGCAA
  CTCATGGTAGAGGGGGTATTCTCGGCCAGTCTGGCAGGGGCGGATTCGGCCGCGGAGGTGGGGACGATGG
  AAGATTGGGCCAGCCTAGAAGGAGCTTTAGAGGTAGGCGAGGCGTCAGGAGAAACACCGTGACCCTCGGC
  AGGATACCACTTGCGGGAGCCCCCGAGATTGGGAACCGGAGCCAGCACAGGTACAATCTGCGGTCATCTG
  GCGCCGCAGGCACTGCCTGTTCCCCAACTCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100386 representing NP_040528
  Red=Cloning sites Green=Tags
  
  MESVEKEDSLTAPFEFATTASTDAANAPTTFPVEAPPLEEEEVIIEQDPGFVSEDDEDRSVPTEDKKQDQ
  DDAEANEEQVGRGDQRHGDYLDVGDDVLLKHLQRQCAIICDALQERSDVPLAIADVSLAYERHLFSPRVP
  PKRQENGTCEPNPRLNFYPVFAVPEVLATYHIFFQNCKIPLSCRANRSRADKQLALRQGAVIPDIASLDE
  VPKIFEGLGRDEKRAANALQQENSENESHCGVLVELEGDNARLAVLKRSIEVTHFAYPALNLPPKVMSTV
  MSELIVRRARPLERDANLQEQTEEGLPAVGDEQLARWLETREPADLEERRKLMMAAVLVTVELECMQRFF
  ADPEMQRKLEETLHYTFRQGYVRQACKISNVELCNLVSYLGILHENRLGQNVLHSTLKGEARRDYVRDCV
  YLFLCYTWQTAMGVWQQCLEERNLKELQKLLKQNLKDLWTAFNERSVAAHLADIIFPERLLKTLQQGLPD
  FTSQSMLQNFRNFILERSGILPATCCALPSDFVPIKYRECPPPLWGHCYLLQLANYLAYHSDIMEDVSGD
  GLLECHCRCNLCTPHRSLVCNSQLLSESQIIGTFELQGPSPDEKSAAPGLKLTPGLWTSAYLRKFVPEDY
  HAHEIRFYEDQSRPPNAELTACVITQGHILGQLQAINKARQEFLLRKGRGVYLDPQSGEELNPIPPPPQP
  YQQPRALASQDGTQKEAAAAAAATHGRGGILGQSGRGGFGRGGGDDGRLGQPRRSFRGRRGVRRNTVTLG
  RIPLAGAPEIGNRSQHRYNLRSSGAAGTACSPTQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040528
• RefSeq ORF: 2415
• Locus ID: 2653005
• MW: 90.1 kDa