E2A (NC_001405) Virus Tagged ORF Clone

CAT#: VC100385

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus C], codon optimized for human cell expression, NP_040527

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100385 represents NCBI reference of NP_040527 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCCGGGAAGAAGAACAACGCGAAACTACTCCGGAGCGCGGCAGAGGCGCTGCAAGAAGGCCAC
  CCACGATGGAAGATGTGAGTAGCCCTTCTCCATCACCCCCACCACCCCGCGCTCCTCCTAAGAAGAGACT
  GAGGCGACGACTGGAATCTGAGGATGAGGAGGACTCAAGCCAAGACGCCCTGGTCCCTCGCACTCCCAGC
  CCCCGGCCTAGCACCAGCACAGCAGATCTTGCCATAGCTAGCAAAAAGAAGAAAAAGAGACCCTCTCCCA
  AGCCTGAGCGCCCTCCCTCTCCAGAGGTGATTGTTGATAGCGAGGAGGAGCGGGAAGATGTAGCCCTCCA
  GATGGTTGGTTTCAGTAACCCGCCAGTGCTGATCAAACACGGAAAAGGCGGGAAGCGGACAGTTCGCAGA
  CTTAATGAAGATGATCCCGTCGCACGAGGCATGCGAACACAGGAGGAAAAAGAAGAGAGTTCCGAGGCAG
  AGTCTGAGAGCACCGTAATCAATCCTTTGAGTCTGCCGATAGTTTCAGCCTGGGAGAAGGGCATGGAAGC
  AGCACGCGCCCTCATGGATAAGTATCACGTAGATAACGACCTCAAAGCTAACTTCAAGCTGCTGCCAGAT
  CAAGTGGAGGCACTTGCCGCTGTGTGCAAAACCTGGCTCAATGAGGAGCATAGAGGACTGCAGCTGACCT
  TTACTTCAAACAAGACATTCGTGACTATGATGGGACGCTTCCTGCAGGCGTACCTGCAGTCATTCGCTGA
  GGTCACATATAAACACCACGAGCCCACCGGCTGTGCATTGTGGCTGCACCGGTGTGCCGAAATCGAGGGC
  GAGCTCAAGTGCCTTCATGGGTCAATAATGATCAACAAGGAACACGTTATCGAGATGGACGTCACCAGCG
  AAAACGGTCAGCGGGCATTGAAAGAGCAGTCTTCAAAAGCCAAGATCGTTAAAAACAGATGGGGACGGAA
  CGTGGTGCAAATCTCCAACACGGACGCTCGATGCTGCGTGCATGATGCTGCATGCCCAGCGAACCAGTTT
  TCTGGGAAATCCTGCGGCATGTTTTTTAGTGAAGGCGCCAAGGCTCAGGTCGCTTTTAAGCAGATCAAGG
  CATTTATGCAAGCCCTCTATCCTAATGCACAGACAGGACACGGTCATCTCCTTATGCCACTTCGCTGTGA
  ATGTAACTCTAAACCCGGGCACGCTCCGTTCCTCGGGCGGCAGCTCCCAAAGCTTACCCCTTTCGCTTTG
  AGTAACGCCGAGGACCTGGATGCCGACCTGATCAGCGACAAATCAGTCCTGGCATCCGTGCATCACCCAG
  CACTCATCGTGTTCCAGTGTTGCAATCCCGTCTACAGAAATTCTAGGGCACAGGGAGGCGGACCGAATTG
  TGACTTTAAAATCAGTGCCCCCGATTTGCTCAATGCCCTGGTTATGGTCAGAAGTCTGTGGTCAGAGAAT
  TTCACCGAGCTTCCTCGCATGGTGGTGCCCGAATTTAAATGGTCCACAAAGCATCAGTACCGCAACGTCA
  GTCTGCCCGTGGCCCACAGCGACGCAAGACAGAATCCATTTGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100385 representing NP_040527
  Red=Cloning sites Green=Tags
  
  MASREEEQRETTPERGRGAARRPPTMEDVSSPSPSPPPPRAPPKKRLRRRLESEDEEDSSQDALVPRTPS
  PRPSTSTADLAIASKKKKKRPSPKPERPPSPEVIVDSEEEREDVALQMVGFSNPPVLIKHGKGGKRTVRR
  LNEDDPVARGMRTQEEKEESSEAESESTVINPLSLPIVSAWEKGMEAARALMDKYHVDNDLKANFKLLPD
  QVEALAAVCKTWLNEEHRGLQLTFTSNKTFVTMMGRFLQAYLQSFAEVTYKHHEPTGCALWLHRCAEIEG
  ELKCLHGSIMINKEHVIEMDVTSENGQRALKEQSSKAKIVKNRWGRNVVQISNTDARCCVHDAACPANQF
  SGKSCGMFFSEGAKAQVAFKQIKAFMQALYPNAQTGHGHLLMPLRCECNSKPGHAPFLGRQLPKLTPFAL
  SNAEDLDADLISDKSVLASVHHPALIVFQCCNPVYRNSRAQGGGPNCDFKISAPDLLNALVMVRSLWSEN
  FTELPRMVVPEFKWSTKHQYRNVSLPVAHSDARQNPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040527
• RefSeq ORF: 1587
• Locus ID: 5739976
• MW: 59.1 kDa