L3 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100383

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus C], codon optimized for human cell expression, NP_040525

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100383 represents NCBI reference of NP_040525 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACTCCCTCCATGATGCCTCAGTGGTCATACATGCACATCTCTGGTCAAGATGCCAGTGAGTACC
  TCTCACCAGGCCTCGTACAGTTTGCAAGGGCCACTGAGACATATTTTAGTCTCAACAACAAATTCCGAAA
  CCCAACCGTGGCCCCTACACATGATGTTACAACAGATCGGAGCCAGCGACTGACGCTCCGGTTCATCCCT
  GTAGATCGGGAGGACACCGCCTACTCTTACAAGGCCAGATTTACTCTCGCTGTGGGAGATAACCGAGTCT
  TGGACATGGCAAGCACTTATTTCGACATTAGAGGCGTTCTGGATAGAGGGCCAACTTTCAAGCCATACTC
  CGGCACCGCTTATAATGCACTGGCACCAAAAGGTGCTCCCAATAGCTGCGAGTGGGAGCAGACTGAGGAT
  TCTGGACGGGCCGTTGCTGAGGACGAAGAAGAAGAGGATGAAGACGAGGAGGAAGAGGAGGAAGAGCAGA
  ACGCTCGGGACCAGGCCACTAAGAAAACTCATGTATATGCCCAGGCCCCTCTGTCTGGCGAGACTATAAC
  TAAGAGCGGTCTCCAGATCGGTTCCGACAACGCAGAGACGCAGGCCAAACCCGTCTATGCCGATCCAAGT
  TACCAGCCAGAACCCCAGATTGGGGAGAGCCAGTGGAATGAAGCCGACGCCAACGCTGCCGGTGGCCGGG
  TGCTCAAAAAGACGACGCCCATGAAACCCTGTTACGGAAGCTACGCTCGGCCCACTAATCCTTTTGGCGG
  GCAGAGCGTCCTCGTACCGGATGAAAAAGGCGTCCCGCTTCCTAAGGTGGACCTGCAATTTTTCTCCAAC
  ACCACTAGCCTGAATGACAGGCAAGGTAACGCCACGAAGCCCAAGGTGGTGCTTTACTCCGAGGATGTCA
  ATATGGAGACGCCCGATACCCATCTTTCCTACAAACCAGGCAAGGGAGATGAAAATTCAAAGGCCATGCT
  GGGCCAGCAAAGCATGCCCAACAGACCCAATTACATTGCTTTCCGAGACAACTTCATCGGACTGATGTAC
  TATAATAGCACCGGCAACATGGGAGTGTTGGCTGGACAGGCTTCCCAGTTGAATGCAGTTGTCGATCTCC
  AGGACCGCAACACTGAACTGAGCTATCAGCTCCTCCTCGATAGTATCGGAGATAGAACCAGGTATTTCTC
  TATGTGGAATCAGGCCGTGGATAGCTACGACCCCGACGTCCGAATTATCGAAAACCACGGCACCGAGGAT
  GAGCTCCCTAACTACTGCTTCCCCCTGGGCGGGATCGGCGTTACCGACACGTACCAAGCAATTAAGGCGA
  ATGGTAATGGAAGCGGGGATAACGGTGACACAACCTGGACAAAAGATGAAACTTTCGCAACCAGAAATGA
  AATTGGGGTGGGGAACAATTTCGCGATGGAAATCAATTTGAACGCTAATCTGTGGAGGAATTTTCTGTAC
  AGCAATATTGCGCTGTATCTGCCCGACAAACTGAAGTACAACCCAACTAATGTAGAGATCAGCGACAATC
  CAAACACTTATGACTACATGAACAAGCGCGTGGTCGCCCCCGGCCTTGTAGACTGTTATATTAACCTCGG
  CGCCCGCTGGAGTTTGGACTACATGGACAACGTGAATCCCTTCAACCACCACCGCAACGCAGGGCTGAGG
  TACAGAAGTATGCTTCTGGGGAATGGCCGGTACGTACCCTTTCATATTCAGGTACCTCAGAAATTCTTTG
  CAATCAAGAATCTTCTCCTCCTGCCAGGTTCCTACACATACGAATGGAATTTCAGGAAGGACGTCAATAT
  GGTGCTGCAGAGCTCACTGGGTAACGACCTGAGAGTTGATGGCGCAAGTATCAAGTTCGATTCCATTTGC
  CTGTACGCTACTTTTTTCCCCATGGCACATAACACCGCCAGCACCTTGGAGGCTATGCTTCGCAACGATA
  CTAATGATCAGTCATTCAATGATTACTTGTCTGCTGCTAACATGCTGTATCCAATCCCGGCTAATGCAAC
  CAATGTCCCGATTAGCATACCGTCCAGGAATTGGGCCGCCTTTCGCGGGTGGGCGTTTACACGGTTGAAG
  ACAAAAGAAACACCTAGTCTGGGTAGTGGCTATGATCCTTATTATACGTATTCCGGCAGCATTCCATATC
  TGGATGGAACATTTTACCTCAACCATACTTTCAAGAAGGTGGCTATTACTTTCGACTCTAGTGTGTCTTG
  GCCGGGAAACGATAGACTTCTGACGCCCAATGAGTTCGAAATTAAGCGCTCTGTGGATGGCGAGGGATAC
  AATGTGGCCCAGTGCAACATGACGAAGGACTGGTTTCTTGTCCAGATGCTGGCAAACTATAACATTGGTT
  ACCAGGGCTTTTATATTCCAGAGTCCTACAAAGACAGAATGTACTCATTTTTTAGGAACTTCCAACCTAT
  GAGCCGCCAGGTTGTGGACGACACTAAGTACAAGGAGTACCAGCAGGTCGGCATACTGCACCAGCACAAT
  AATTCTGGCTTCGTCGGGTACCTGGCCCCAACCATGCGGGAGGGCCAGGCGTACCCTGCCAATGTGCCCT
  ATCCTCTGATTGGCAAAACCGCGGTGGACAGCATTACCCAAAAAAAGTTCCTGTGCGACAGGACCCTGTG
  GAGAATTCCCTTCTCAAGTAATTTCATGAGCATGGGAGCCCTGACCGACCTTGGCCAAAACCTGCTCTAC
  GCAAACTCAGCACACGCTCTGGACATGACCTTCGAAGTTGACCCAATGGATGAACCCACGCTGCTGTACG
  TACTGTTTGAGGTTTTTGATGTGGTGCGGGTCCATCAGCCTCACCGCGGCGTGATAGAGACGGTTTATCT
  GCGAACACCTTTTTCCGCCGGCAATGCAACCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100383 representing NP_040525
  Red=Cloning sites Green=Tags
  
  MATPSMMPQWSYMHISGQDASEYLSPGLVQFARATETYFSLNNKFRNPTVAPTHDVTTDRSQRLTLRFIP
  VDREDTAYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPTFKPYSGTAYNALAPKGAPNSCEWEQTED
  SGRAVAEDEEEEDEDEEEEEEEQNARDQATKKTHVYAQAPLSGETITKSGLQIGSDNAETQAKPVYADPS
  YQPEPQIGESQWNEADANAAGGRVLKKTTPMKPCYGSYARPTNPFGGQSVLVPDEKGVPLPKVDLQFFSN
  TTSLNDRQGNATKPKVVLYSEDVNMETPDTHLSYKPGKGDENSKAMLGQQSMPNRPNYIAFRDNFIGLMY
  YNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLLLDSIGDRTRYFSMWNQAVDSYDPDVRIIENHGTED
  ELPNYCFPLGGIGVTDTYQAIKANGNGSGDNGDTTWTKDETFATRNEIGVGNNFAMEINLNANLWRNFLY
  SNIALYLPDKLKYNPTNVEISDNPNTYDYMNKRVVAPGLVDCYINLGARWSLDYMDNVNPFNHHRNAGLR
  YRSMLLGNGRYVPFHIQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASIKFDSIC
  LYATFFPMAHNTASTLEAMLRNDTNDQSFNDYLSAANMLYPIPANATNVPISIPSRNWAAFRGWAFTRLK
  TKETPSLGSGYDPYYTYSGSIPYLDGTFYLNHTFKKVAITFDSSVSWPGNDRLLTPNEFEIKRSVDGEGY
  NVAQCNMTKDWFLVQMLANYNIGYQGFYIPESYKDRMYSFFRNFQPMSRQVVDDTKYKEYQQVGILHQHN
  NSGFVGYLAPTMREGQAYPANVPYPLIGKTAVDSITQKKFLCDRTLWRIPFSSNFMSMGALTDLGQNLLY
  ANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRVHQPHRGVIETVYLRTPFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 2904 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040525
• RefSeq ORF: 2904
• Locus ID: 2652998
• MW: 109.2 kDa