E4 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100401

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf4 [Human adenovirus C], codon optimized for human cell expression, YP_001551773

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100401 represents NCBI reference of YP_001551773 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTGCCAGCTCTCCCAGCCCCCCCAGTGTGTGACTCACAGAACGAATGTGTTGGATGGCTGGGGG
  TGGCCTACTCCGCCGTGGTGGACGTGATCCGCGCAGCTGCACATGAAGGCGTTTATATTGAACCTGAGGC
  CAGAGGTCGACTGGACGCACTGCGGGAGTGGATTTACTATAACTACTATACAGAACGCGCCAAGAGGAGG
  GATAGACGCCGGAGATCAGTGTGTCACGCCCGGACCTGGTTTTGCTTTCGGAAATATGACTACGTGCGCA
  GGTCTATCTGGCATGACACAACTACAAACACCATCAGCGTGGTAAGCGCCCACTCTGTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100401 representing YP_001551773
  Red=Cloning sites Green=Tags
  
  MVLPALPAPPVCDSQNECVGWLGVAYSAVVDVIRAAAHEGVYIEPEARGRLDALREWIYYNYYTERAKRR
  DRRRRSVCHARTWFCFRKYDYVRRSIWHDTTTNTISVVSAHSVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 342 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, YP_001551773
• RefSeq ORF: 342
• Locus ID: 2652989
• MW: 13.3 kDa