L3 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100382

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVI [Human adenovirus C], codon optimized for human cell expression, NP_040524

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100382 represents NCBI reference of NP_040524 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACATCAATTTTGCATCACTCGCCCCTAGACACGGCAGCAGGCCCTTCATGGGCAATTGGCAGG
  ACATTGGTACATCAAATATGTCCGGTGGCGCTTTCTCTTGGGGCTCCCTCTGGTCTGGTATCAAAAACTT
  CGGAAGTACAATAAAGAATTATGGCTCAAAGGCATGGAACAGTAGTACAGGGCAGATGCTTCGAGATAAG
  CTGAAGGAACAGAACTTTCAACAAAAGGTTGTGGACGGTTTGGCTTCTGGGATTTCCGGCGTGGTGGATC
  TGGCAAACCAGGCTGTGCAAAATAAGATCAACTCTAAACTGGATCCGCGACCACCGGTAGAGGAGCCCCC
  CCCCGCCGTGGAAACCGTATCACCTGAAGGCAGGGGAGAGAAGCGGCCAAGGCCGGATAGGGAAGAGACA
  CTGGTAACCCAAATTGATGAACCACCGTCTTACGAAGAAGCACTGAAACAAGGGCTGCCCACCACTCGGC
  CAATTGCCCCGATGGCGACCGGAGTGCTCGGCCAGCATACTCCGGTAACGCTTGACCTCCCGCCCCCAGC
  GGATACACAACAGAAACCTGTGTTGCCTGGCCCTTCAGCCGTCGTGGTGACTCGACCTTCCCGCGCATCA
  CTCAGACGCGCCGCCTCAGGACCCAGATCTATGCGCCCAGTGGCGAGTGGCAACTGGCAATCAACCTTGA
  ACTCTATCGTTGGGCTCGGAGTGCAAAGTCTCAAACGCCGCCGCTGTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100382 representing NP_040524
  Red=Cloning sites Green=Tags
  
  MEDINFASLAPRHGSRPFMGNWQDIGTSNMSGGAFSWGSLWSGIKNFGSTIKNYGSKAWNSSTGQMLRDK
  LKEQNFQQKVVDGLASGISGVVDLANQAVQNKINSKLDPRPPVEEPPPAVETVSPEGRGEKRPRPDREET
  LVTQIDEPPSYEEALKQGLPTTRPIAPMATGVLGQHTPVTLDLPPPADTQQKPVLPGPSAVVVTRPSRAS
  LRRAASGPRSMRPVASGNWQSTLNSIVGLGVQSLKRRRCF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040524
• RefSeq ORF: 750
• Locus ID: 2652998
• MW: 27.0 kDa