E3A (NC_001405) Virus Tagged ORF Clone

CAT#: VC100392

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 gp19K [Human adenovirus C], codon optimized for human cell expression, NP_040531

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100392 represents NCBI reference of NP_040531 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTATATGATCCTGGGACTGCTGGCTCTTGCTGCAGTTTGTAGCGCCGCCAAAAAAGTGGAATTTA
  AGGAGCCAGCCTGCAATGTTACATTCAAAAGCGAGGCCAACGAGTGCACCACTCTGATAAAATGTACGAC
  CGAGCACGAAAAATTGATCATCCGCCATAAGGATAAGATCGGCAAATACGCTGTATACGCAATATGGCAG
  CCTGGTGATACCAACGATTACAACGTCACCGTGTTTCAGGGAGAGAATAGGAAGACCTTTATGTATAAGT
  TTCCATTCTATGAGATGTGTGATATTACTATGTACATGAGCAAACAGTACAAGCTTTGGCCACCTCAGAA
  GTGTCTGGAAAACACTGGTACATTCTGTTCTACCGCCCTGCTGATTACTGCACTGGCTCTCGTTTGCACC
  CTCCTCTACTTGAAGTATAAAAGCCGGCGGAGTTTCATAGACGAAAAGAAAATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100392 representing NP_040531
  Red=Cloning sites Green=Tags
  
  MRYMILGLLALAAVCSAAKKVEFKEPACNVTFKSEANECTTLIKCTTEHEKLIIRHKDKIGKYAVYAIWQ
  PGDTNDYNVTVFQGENRKTFMYKFPFYEMCDITMYMSKQYKLWPPQKCLENTGTFCSTALLITALALVCT
  LLYLKYKSRRSFIDEKKMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 477 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040531
• RefSeq ORF: 477
• Locus ID: 2652987
• MW: 18.4 kDa