L5 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100398

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus C], codon optimized for human cell expression, NP_040533

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100398 represents NCBI reference of NP_040533 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAGAGCCAGACCAAGCGAGGACACTTTTAATCCCGTCTACCCCTATGATACGGAGACAGGACCAC
  CTACCGTGCCTTTCCTGACTCCTCCATTTGTCTCACCGAACGGGTTCCAGGAGTCTCCACCAGGAGTGCT
  TAGCCTGCGCGTCTCTGAGCCACTGGACACCTCTCACGGAATGCTTGCCCTTAAAATGGGGAGCGGCCTG
  ACACTGGATAAGGCGGGCAATCTGACTTCCCAGAACGTGACGACGGTAACCCAGCCCCTGAAGAAGACTA
  AGTCCAATATTTCCCTGGATACTTCTGCCCCGCTGACGATAACTAGCGGAGCATTGACCGTTGCCACGAC
  TGCTCCACTCATCGTGACAAGCGGGGCGCTCTCTGTGCAGAGCCAAGCCCCACTCACCGTGCAGGACTCT
  AAACTGTCCATTGCCACTAAAGGACCCATCACTGTGTCCGACGGAAAACTTGCCCTCCAAACAAGTGCTC
  CTCTGTCAGGTAGTGACAGTGACACTTTGACCGTGACCGCATCACCCCCCCTCACAACCGCGACAGGTTC
  CCTTGGCATCAATATGGAAGATCCAATCTACGTGAATAACGGCAAAATTGGCATCAAAATCTCAGGCCCA
  CTGCAGGTTGCCCAAAATAGCGACACCCTGACCGTTGTCACAGGCCCTGGCGTCACAGTAGAGCAGAACT
  CCCTTAGAACTAAGGTGGCCGGAGCTATCGGATATGACTCATCAAATAACATGGAAATCAAAACCGGAGG
  GGGTATGAGAATCAATAATAATCTGTTGATCCTGGATGTTGATTATCCCTTCGACGCACAAACAAAGCTG
  CGCCTGAAACTGGGGCAAGGTCCCTTGTACATAAATGCAAGTCATAATCTCGATATTAACTACAATCGCG
  GTCTTTATCTTTTCAACGCATCAAACAATACAAAGAAACTGGAAGTGTCTATTAAAAAAAGTAGCGGACT
  GAACTTTGACAACACCGCAATAGCCATAAATGCCGGAAAAGGATTGGAATTCGATACAAATACGTCCGAG
  TCCCCCGATATTAACCCCATTAAGACTAAGATCGGTTCCGGTATTGATTACAACGAAAATGGGGCTATGA
  TTACCAAGCTGGGCGCCGGCCTCAGCTTCGATAATTCCGGGGCCATCACCATCGGTAACAAGAATGATGA
  CAAACTCACCCTGTGGACCACACCAGACCCAAGTCCGAACTGTCGAATCCACTCAGACAATGACTGCAAA
  TTCACATTGGTCTTGACGAAATGCGGCAGTCAGGTCCTTGCTACTGTTGCAGCCCTCGCCGTGAGCGGGG
  ACCTGAGCAGCATGACAGGGACGGTTGCGAGTGTGAGCATATTTCTGCGGTTCGATCAGAACGGCGTGCT
  CATGGAAAACTCCTCCCTCAAGAAGCACTATTGGAATTTTCGAAATGGGAACAGCACAAATGCTAACCCT
  TACACCAACGCGGTCGGATTCATGCCCAATTTGCTCGCATATCCAAAAACCCAGTCCCAAACTGCAAAGA
  ACAATATCGTGTCCCAGGTGTACTTGCACGGAGATAAAACCAAGCCCATGATTCTGACCATAACTCTCAA
  TGGTACCAGCGAAAGTACCGAAACAAGCGAAGTCTCTACCTATTCTATGTCATTTACATGGTCATGGGAA
  TCTGGCAAGTACACCACGGAAACCTTCGCCACCAACAGCTATACTTTTTCCTATATCGCACAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100398 representing NP_040533
  Red=Cloning sites Green=Tags
  
  MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPGVLSLRVSEPLDTSHGMLALKMGSGL
  TLDKAGNLTSQNVTTVTQPLKKTKSNISLDTSAPLTITSGALTVATTAPLIVTSGALSVQSQAPLTVQDS
  KLSIATKGPITVSDGKLALQTSAPLSGSDSDTLTVTASPPLTTATGSLGINMEDPIYVNNGKIGIKISGP
  LQVAQNSDTLTVVTGPGVTVEQNSLRTKVAGAIGYDSSNNMEIKTGGGMRINNNLLILDVDYPFDAQTKL
  RLKLGQGPLYINASHNLDINYNRGLYLFNASNNTKKLEVSIKKSSGLNFDNTAIAINAGKGLEFDTNTSE
  SPDINPIKTKIGSGIDYNENGAMITKLGAGLSFDNSGAITIGNKNDDKLTLWTTPDPSPNCRIHSDNDCK
  FTLVLTKCGSQVLATVAALAVSGDLSSMTGTVASVSIFLRFDQNGVLMENSSLKKHYWNFRNGNSTNANP
  YTNAVGFMPNLLAYPKTQSQTAKNNIVSQVYLHGDKTKPMILTITLNGTSESTETSEVSTYSMSFTWSWE
  SGKYTTETFATNSYTFSYIAQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 1746 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040533
• RefSeq ORF: 1746
• Locus ID: 2652999
• MW: 61.9 kDa