E1B (NC_001405) Virus Tagged ORF Clone

CAT#: VC100370

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 55K [Human adenovirus C], codon optimized for human cell expression, NP_040511

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100370 represents NCBI reference of NP_040511 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGCGCAATCCTTCTGAGAGGGGAGTGCCCGCCGGTTTCTCAGGGCATGCATCCGTGGAGTCCG
  GGGGGGAGACACAAGAATCACCTGCAACAGTGGTGTTCCGGCCCCCTGGCAATAATACAGACGGGGGCGC
  CACTGCAGGAGGCTCCCAAGCTGCAGCTGCTGCCGGTGCTGAACCGATGGAGCCCGAAAGCAGGCCAGGG
  CCTTCAGGTATGAACGTGGTGCAGGTGGCTGAACTGTTCCCAGAACTGCGACGGATCCTTACCATTAACG
  AGGATGGGCAAGGTCTCAAGGGAGTCAAGAGGGAACGAGGAGCCAGCGAAGCGACCGAGGAAGCAAGGAA
  CCTCACATTTTCTCTGATGACCAGACACAGGCCGGAGTGTGTAACATTCCAGCAGATAAAAGACAATTGC
  GCCAATGAGCTGGACCTCCTCGCCCAGAAGTACAGTATAGAGCAGCTCACCACATACTGGCTGCAGCCAG
  GCGACGATTTCGAGGAGGCCATAAGAGTCTACGCCAAGGTGGCCCTGAGACCTGATTGTAAATACAAAAT
  TTCCAAGCTGGTCAACATCCGAAACTGTTGCTATATCAGTGGGAATGGCGCTGAGGTGGAGATCGATACA
  GAGGACCGAGTGGCCTTTCGATGTAGTATGATCAACATGTGGCCTGGGGTGCTTGGGATGGATGGTGTGG
  TTATTATGAACGTCCGATTTACTGGACCTAATTTCTCCGGGACCGTGTTTCTCGCAAACACCAACCTCAT
  TTTGCATGGGGTGTCATTTTATGGGTTTAACAATACTTGTGTCGAGGCCTGGACGGATGTAAGAGTGCGG
  GGATGCGCATTCTACTGCTGTTGGAAGGGCGTTGTGTGCCGACCAAAAAGTCGCGCGTCAATTAAGAAGT
  GTCTCTTCGAACGGTGCACCCTGGGGATTCTCAGCGAAGGAAACTCCCGCGTGCGCCATAACGTGGCATC
  CGATTGCGGTTGCTTTATGCTGGTCAAAAGTGTCGCCGTGATTAAACACAACATGGTCTGTGGCAATTGT
  GAGGATCGCGCTTCCCAAATGCTCACATGCTCTGATGGAAATTGTCATCTGCTGAAGACAATCCACGTCG
  CTAGCCACTCTCGCAAGGCCTGGCCCGTCTTCGAGCATAACATCCTCACACGGTGTAGCCTCCACCTGGG
  CAACAGACGAGGTGTCTTCCTGCCATACCAATGTAATCTCTCCCACACCAAGATACTGCTGGAACCTGAA
  TCCATGTCAAAAGTGAATCTGAACGGCGTGTTTGATATGACGATGAAAATATGGAAAGTCCTCCGATATG
  ATGAGACTCGGACTAGATGCCGGCCTTGCGAGTGTGGTGGTAAGCATATCCGCAACCAGCCCGTAATGCT
  TGACGTGACCGAAGAACTGCGCCCCGACCACCTGGTACTCGCATGCACCAGAGCGGAATTTGGTTCTTCC
  GATGAAGACACCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100370 representing NP_040511
  Red=Cloning sites Green=Tags
  
  MERRNPSERGVPAGFSGHASVESGGETQESPATVVFRPPGNNTDGGATAGGSQAAAAAGAEPMEPESRPG
  PSGMNVVQVAELFPELRRILTINEDGQGLKGVKRERGASEATEEARNLTFSLMTRHRPECVTFQQIKDNC
  ANELDLLAQKYSIEQLTTYWLQPGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAEVEIDT
  EDRVAFRCSMINMWPGVLGMDGVVIMNVRFTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWTDVRVR
  GCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNSRVRHNVASDCGCFMLVKSVAVIKHNMVCGNC
  EDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGNRRGVFLPYQCNLSHTKILLEPE
  SMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGGKHIRNQPVMLDVTEELRPDHLVLACTRAEFGSS
  DEDTD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040511
• RefSeq ORF: 1485
• Locus ID: 2652981
• MW: 54.9 kDa