E4 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100402

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf3 [Human adenovirus C], codon optimized for human cell expression, NP_040534

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100402 represents NCBI reference of NP_040534 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTAGATGTTTGCGGCTGAAGGTGGAGGGAGCTCTTGAACAGATCTTTACGATGGCGGGGCTCAACA
  TCAGGGATTTGCTGAGAGATATCCTGATCAGGTGGCGCGACGAAAACTATCTGGGTATGGTGGAAGGGGC
  TGGGATGTTTATTGAAGAGATACACCCCGAGGGATTCTCCCTCTATGTCCACCTGGATGTTCGAGCTGTA
  TGTCTGCTCGAGGCTATCGTCCAGCATCTGACCAATGCAATTATTTGTTCTCTCGCTGTCGAATTCGATC
  ATGCTACCGGAGGCGAAAGAGTGCACTTGATCGACTTGCACTTTGAGGTGCTGGATAATCTTCTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100402 representing NP_040534
  Red=Cloning sites Green=Tags
  
  MIRCLRLKVEGALEQIFTMAGLNIRDLLRDILIRWRDENYLGMVEGAGMFIEEIHPEGFSLYVHLDVRAV
  CLLEAIVQHLTNAIICSLAVEFDHATGGERVHLIDLHFEVLDNLLE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 348 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040534
• RefSeq ORF: 348
• Locus ID: 2652989
• MW: 13.3 kDa