E2B (NC_001405) Virus Tagged ORF Clone

CAT#: VC100373

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human adenovirus C], codon optimized for human cell expression, NP_040516

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100373 represents NCBI reference of NP_040516 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGTTCAGGCTCATCGGGCTAGACGGCTGCATGCTGAGGCTCCTGATAGTGGCGACCAACCAC
  CAAGGAGACGAGTAAGACAGCAGCCTCCCCGCGCTGCACCAGCCCCCGCTAGAGCCCGAAGACGGCGAGC
  ACCTGCACCATCTCCCGGAGGAAGTAGAGCTCCACCAACCAGTGGAGGCCCTCCCGCCAGTCCGCTTCTC
  GATGCAAGCAGCAAGGATACCCCTGCTGCGCATAGACCACCCAGGGGTACCGTTGTGGCTCCGCGGGGGT
  GTGGCCTGTTGCAGGTCATCGACGCCGCAACCAACCAACCACTCGAGATTAGGTATCATTTGGACCTCGC
  CCGCGCTCTGACTAGGCTGTGTGAAGTGAATCTGCAGGAGCTGCCACCCGACCTTAGCCCAAGAGAATTG
  CAAACCATGGACTCTTCACACCTGCGGGACGTGGTGATCAAACTGAGGCCCCCACGCGCAGATATCTGGA
  CACTGGGCAGCAGAGGCGTAGTGGTCAGAAGTACCATCACACCACTGGAGCAGCCCGATGGGCAGGGACA
  AGCCGCTGAAGTCGAGGACCACCAACCCAATCCTCCCGGCGAGGGCCTCAAGTTCCCTTTGTGCTTCCTC
  GTAAGAGGCAGACAAGTAAATCTTGTGCAGGACGTTCAGCCTGTTCACAGGTGTCAGTACTGCGCGAGGT
  TTTATAAGAGCCAGCACGAGTGTAGTGCTAGAAGAAGGGATTTTTACTTCCATCACATTAATTCACACTC
  AAGTAATTGGTGGCGCGAGATTCAATTCTTCCCTATCGGGAGCCACCCACGGACCGAGAGGCTTTTTGTG
  ACCTACGACGTGGAAACCTACACCTGGATGGGAGCGTTTGGAAAGCAACTCGTACCCTTCATGCTTGTTA
  TGAAATTTGGCGGAGATGAGCCACTCGTCACCGCCGCTCGAGATCTCGCAGTTGATCTTGGATGGGATAG
  ATGGGAGCAGGATCCTCTGACATTTTATTGCATCACGCCCGAAAAAATGGCCATTGGAAGACAGTTTCGG
  ACCTTCCGAGATCATCTCCAGATGTTGATGGCGCGGGACCTGTGGTCTTCCTTCGTGGCTTCTAACCCAC
  ATCTGGCCGATTGGGCGCTTAGTGAGCATGGACTGAGTTCTCCCGAGGAGCTGACGTATGAAGAACTGAA
  GAAGCTCCCCAGTATCAAAGGAACTCCTCGGTTTCTGGAGCTTTATATAGTGGGGCATAACATTAATGGG
  TTTGACGAGATCGTGCTTGCTGCACAGGTGATCAACAACCGGTCAGAGGTGCCTGGCCCATTTAGGATTA
  CCAGGAACTTTATGCCCAGAGCCGGTAAGATATTGTTCAACGACGTGACTTTTGCTTTGCCGAACCCAAG
  AAGCAAGAAGCGGACTGACTTCCTCCTGTGGGAACAAGGGGGCTGTGATGACACAGATTTCAAATATCAG
  TACCTTAAGGTTATGGTGCGCGACACCTTCGCCCTGACTCATACATCACTGAGGAAAGCCGCTCAGGCCT
  ACGCACTGCCCGTAGAAAAGGGGTGCTGCGCCTATCAAGCCGTGAACCAGTTCTATATGCTGGGCAGTTA
  TAGGAGTGAGGCTGATGGCTTTCCTATTCAGGAGTATTGGAAGGATCGAGAGGAATTTGTGCTGAATAGA
  GAGCTGTGGAAGAAGAAGGGGCAGGACAAATACGACATTATTAAGGAGACTTTGGATTACTGTGCTCTGG
  ATGTGCAGGTAACCGCAGAGCTTGTTAACAAACTTCGGGACTCTTACGCTTCATTCGTGCGAGATGCAGT
  CGGTCTTACCGACGCCTCCTTCAATGTATTCCAACGACCTACCATATCCTCTAATTCTCACGCTATCTTC
  CGCCAGATAGTCTTTCGCGCTGAGCAGCCAGCTAGATCAAATCTGGGGCCTGATCTCCTCGCCCCCTCCC
  ATGAACTGTATGACTATGTTAGGGCCAGCATTCGGGGCGGCCGGTGCTATCCCACCTACCTGGGGATCCT
  GAGAGAGCCTTTGTACGTATATGACATATGCGGTATGTACGCCTCCGCCCTGACCCACCCCATGCCATGG
  GGTCCTCCGCTCAACCCGTATGAACGCGCCCTCGCCGCCAGAGCTTGGCAGCAAGCACTGGACCTGCAGG
  GATGTAAGATCGATTACTTCGATGCTCGGCTGCTTCCCGGTGTCTTCACAGTGGACGCAGACCCACCCGA
  TGAAACCCAACTTGATCCACTGCCCCCGTTCTGCTCAAGAAAAGGGGGCCGGCTGTGTTGGACCAACGAG
  AGGCTGAGGGGTGAGGTGGCCACTAGCGTGGATCTGGTGACGCTCCACAACAGGGGATGGCGCGTGCACC
  TGGTCCCTGACGAACGCACAACTGTGTTCCCAGAATGGCGGTGCGTGGCTAGAGAATATGTGCAGCTGAA
  CATTGCAGCTAAAGAGCGAGCCGATAGAGACAAAAACCAGACCCTTAGGTCAATCGCCAAGCTGCTCTCT
  AATGCCCTGTATGGAAGCTTCGCTACTAAACTCGACAATAAGAAGATAGTGTTCTCTGACCAAATGGACG
  CCGCGACCCTCAAAGGAATCACAGCGGGCCAAGTGAACATTAAGAGTAGCAGCTTTCTTGAAACAGACAA
  CCTCAGTGCCGAAGTGATGCCCGCCTTCGAACGGGAATATAGCCCTCAGCAGCTGGCCCTGGCAGATTCA
  GACGCAGAGGAGTCAGAGGACGAGAGAGCGCCCACACCGTTTTATAGCCCTCCAAGCGGTACTCCTGGGC
  ACGTCGCATACACGTATAAACCGATCACTTTCCTCGATGCGGAGGAGGGGGACATGTGCCTGCACACGCT
  CGAAAGAGTGGATCCACTCGTGGATAATGACCGATACCCTAGCCACCTGGCCAGTTTCGTTCTTGCTTGG
  ACTCGAGCCTTTGTGAGTGAGTGGTCCGAATTCCTGTATGAGGAGGATAGAGGAACCCCTTTGGAAGACC
  GGCCCCTCAAGAGTGTTTACGGCGATACTGACAGCCTGTTCGTGACAGAAAGAGGGCACCGGCTGATGGA
  GACAAGAGGGAAGAAAAGGATCAAGAAACATGGCGGTAATCTTGTATTCGATCCTGAGCGCCCGGAACTT
  ACTTGGCTGGTGGAGTGTGAAACTGTGTGCGGGGCCTGTGGTGCTGACGCTTATTCTCCAGAGAGCGTAT
  TTCTGGCCCCCAAGCTGTACGCACTTAAAAGCCTCCATTGTCCTTCTTGTGGGGCGTCTTCTAAAGGTAA
  GTTGCGCGCGAAGGGCCATGCCGCCGAGGGTCTGGATTACGACACGATGGTAAAATGTTACCTGGCAGAC
  GCACAGGGCGAAGATAGACAGCGGTTCAGCACAAGTCGGACATCTCTCAAAAGGACTCTGGCATCCGCTC
  AGCCTGGTGCTCACCCCTTTACCGTGACTCAGACTACCTTGACCAGAACATTGCGCCCGTGGAAGGACAT
  GACCCTGGCGCGACTCGATGAGCACAGACTCCTGCCTTATAGTGAGTCCAGACCTAACCCGCGAAATGAG
  GAGATCTGTTGGATAGAAATGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100373 representing NP_040516
  Red=Cloning sites Green=Tags
  
  MALVQAHRARRLHAEAPDSGDQPPRRRVRQQPPRAAPAPARARRRRAPAPSPGGSRAPPTSGGPPASPLL
  DASSKDTPAAHRPPRGTVVAPRGCGLLQVIDAATNQPLEIRYHLDLARALTRLCEVNLQELPPDLSPREL
  QTMDSSHLRDVVIKLRPPRADIWTLGSRGVVVRSTITPLEQPDGQGQAAEVEDHQPNPPGEGLKFPLCFL
  VRGRQVNLVQDVQPVHRCQYCARFYKSQHECSARRRDFYFHHINSHSSNWWREIQFFPIGSHPRTERLFV
  TYDVETYTWMGAFGKQLVPFMLVMKFGGDEPLVTAARDLAVDLGWDRWEQDPLTFYCITPEKMAIGRQFR
  TFRDHLQMLMARDLWSSFVASNPHLADWALSEHGLSSPEELTYEELKKLPSIKGTPRFLELYIVGHNING
  FDEIVLAAQVINNRSEVPGPFRITRNFMPRAGKILFNDVTFALPNPRSKKRTDFLLWEQGGCDDTDFKYQ
  YLKVMVRDTFALTHTSLRKAAQAYALPVEKGCCAYQAVNQFYMLGSYRSEADGFPIQEYWKDREEFVLNR
  ELWKKKGQDKYDIIKETLDYCALDVQVTAELVNKLRDSYASFVRDAVGLTDASFNVFQRPTISSNSHAIF
  RQIVFRAEQPARSNLGPDLLAPSHELYDYVRASIRGGRCYPTYLGILREPLYVYDICGMYASALTHPMPW
  GPPLNPYERALAARAWQQALDLQGCKIDYFDARLLPGVFTVDADPPDETQLDPLPPFCSRKGGRLCWTNE
  RLRGEVATSVDLVTLHNRGWRVHLVPDERTTVFPEWRCVAREYVQLNIAAKERADRDKNQTLRSIAKLLS
  NALYGSFATKLDNKKIVFSDQMDAATLKGITAGQVNIKSSSFLETDNLSAEVMPAFEREYSPQQLALADS
  DAEESEDERAPTPFYSPPSGTPGHVAYTYKPITFLDAEEGDMCLHTLERVDPLVDNDRYPSHLASFVLAW
  TRAFVSEWSEFLYEEDRGTPLEDRPLKSVYGDTDSLFVTERGHRLMETRGKKRIKKHGGNLVFDPERPEL
  TWLVECETVCGACGADAYSPESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLAD
  AQGEDRQRFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRPNPRNE
  EICWIEMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 3594 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, NP_040516
• RefSeq ORF: 3594
• Locus ID: 2652985
• MW: 135.7 kDa