E4 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100403

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf2 [Human adenovirus C], codon optimized for human cell expression, YP_001551774

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100403 represents NCBI reference of YP_001551774 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGAGAGAAAGATGGTATCATTTTCCGTCGTCGTACCCGAGTTGACCTGCCTGTACCTGCATGAAC
  ATGATTACGACGTCCTGGCCTTTCTTAGGGAAGCCCTCCCTGATTTTCTCTCAAGTACATTGCACTTTAT
  CAGCCCACCGATGCAGCAGGCATATATTGGGGCCACTCTCGTATCTATTGCCCCTTCTATGCGGGTGATT
  ATCTCCGTCGGCTCTTTTGTAATGGTTCCAGGCGGCGAAGTCGCAGCACTTGTGCGGGCAGATCTGCATG
  ACTATGTGCAGCTGGCGCTCAGGAGGGATCTGCGCGATAGGGGCATCTTTGTTAATGTTCCTCTGCTCAA
  TCTGATACAGGTATGCGAAGAGCCCGAGTTCTTGCAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100403 representing YP_001551774
  Red=Cloning sites Green=Tags
  
  MFERKMVSFSVVVPELTCLYLHEHDYDVLAFLREALPDFLSSTLHFISPPMQQAYIGATLVSIAPSMRVI
  ISVGSFVMVPGGEVAALVRADLHDYVQLALRRDLRDRGIFVNVPLLNLIQVCEEPEFLQS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001405.1, YP_001551774
• RefSeq ORF: 390
• Locus ID: 2652989
• MW: 14.6 kDa