Secondary Antibodies

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Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:40,000 to 1:200,000 is suggested for this product.
The following general recommendations are suggested as starting dilutions for:
Immunoblotting: 1:10,000,
Enzyme Immunohisto-chemistry on tissue sections: 1:500-1:5,000.
Flow cytometry and fluorescence Immunohisto/cytochemistry: 1:200-1:1,000.
Conjugation Biotin
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s)
FLISA:  1/10,000 - 1/50,000
IF Microscopy:  1/1000- 1/5000
Conjugation FITC
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Conjugation HRP
Immunogen Goat IgG whole molecule

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA: 1:5,000 - 1:20,000.
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
Conjugation Texas Red
Immunogen Goat IgG whole molecule.

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:3,000 - 1:12,000.
Western Blot: 1:500 - 1:2,000.
Conjugation AP
Immunogen Golden Syrian Hamster IgG whole molecule

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
ELISA: 1:20,000 - 1:100,000.
Western Blot: 1:2,000 - 1:10,000.
Conjugation Biotin
Immunogen Golden Syrian Hamster IgG whole molecule

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
Conjugation FITC
Immunogen Golden Syrian Hamster IgG whole molecule.

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:4,000 - 1:16,000.
Western Blot: 1:500 - 1:2,000.
Conjugation HRP
Immunogen Golden Syrian Hamster IgG whole molecule

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1:20,000 - 1:100,000
Western Blot: 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Immunogen Golden Syrian Hamster IgG whole molecule.

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
Conjugation TRITC
Immunogen Golden Syrian Hamster IgG whole molecule.

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000-1:10,000.
Immunofluorescence microscopy: 1:500-1:2,500.
Conjugation Texas Red
Immunogen Golden Syrian Hamster IgG whole molecule.

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, AP

Applications ELISA: 1:4,000 - 1:20,000.
Western blot: 1:1,000 - 1:5,000.
Immunohistochemistry: 1:200 - 1:1,000.
Conjugation AP
Immunogen Mouse IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:7,000 to 1:70,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Mouse IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation FITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Mouse IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
ELISA: 1:2,000 - 1:20,000.
Western blot: 1:1,000 - 1:10,000.
Immunogen Mouse IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, PE

Applications Immunofluorescence: 1/100 - 1/250
Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE
Immunogen Mouse IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation TRITC
Immunogen Mouse IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation Texas Red
Immunogen Mouse IgG whole molecule

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s): This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Rat IgG whole molecule.

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s): This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested.
Conjugation Biotin
Immunogen Rat IgG whole molecule

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s): This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Rat IgG whole molecule

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommende Dilutions:
ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Immunogen Rat IgG whole molecule.

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation TRITC
Immunogen Rat IgG whole molecule.

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation Texas Red
Immunogen Rat IgG whole molecule.

Sheep IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/25,000.
Western Blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Sheep IgG whole molecule

Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:80,000 is suggested for this product.
Conjugation Biotin
Immunogen Sheep IgG whole molecule

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation FITC
Immunogen Sheep IgG whole molecule.

Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/400,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Sheep IgG whole molecule.

Sheep IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA : 1 :20,000-1 :100,000
Western blot : 1 :2,000-1 :10,000
Immunohistochemistry : 1 :1,000-1 :5,000.
Immunogen Sheep IgG whole molecule

Sheep IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation TRITC
Immunogen Sheep IgG whole molecule.

Sheep IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Sheep IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/25-1/100 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC
Immunogen Purified Mouse IgG. 
Antisera to Mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen.

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/500 - 1/1000.
Western Blot: 1/2000 - 1/5000.
Immunohistochemistry on frozen, paraffin and resin sections: 1/50.
Reactivities Mouse
Conjugation HRP
Immunogen Purified whole mouse immunoglobulin G

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Purified

Applications ELISA: 1/500 - 1/1000.
Immunohistochemistry on frozen and paraffin sections: 1/100 - 1/500.
Reactivities Mouse
Immunogen Purified mouse IgG

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/20 diluted anti body to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE
Immunogen Purified mouse IgG

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, FITC

Applications Flow Cytometry: 1/200 - 1/400; Use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/2000.
Immunohistochemistry on frozen sections: 1/50.
Immunohistochemistry on paraffin sections: 1/50.
Immunohistochemistry on resin sections: 1/50.
Reactivities Rat
Conjugation HRP
Immunogen Purified rat immunoglobulin G

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, Purified

Applications ELISA: 1/250 - 1/1000.
Immunodiffusion.
Reactivities Rat
Immunogen Purified polyclonal rat IgG

Sheep IgA rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Sheep

Sheep IgM rabbit polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2000.
Reactivities Sheep
Conjugation FITC

Sheep IgM rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Sheep
Conjugation HRP

Sheep IgM rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Sheep

FITC Conjugated Rabbit Anti-Rat IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Rat IgG
Conjugation FITC
Immunogen Rat IgG, whole molecule

Cy3 Conjugated Rabbit Anti-Human IgG

Applications IF/ICC: 1:50-100; IHC: 1:50-100
Reactivities Human IgG
Conjugation Cy3
Immunogen Human IgG, whole molecule

Goat anti-rabbit IgG Antiserum, pretitered, polyclonal

Reactivities Human, Mammalian
Conjugation Unconjugated