Secondary Antibodies

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Peroxidase Conjugated Goat Anti-Rabbit IgG (Min Hu, Ms)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP

Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP

Goat Anti-Rabbit IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Rabbit IgG
Conjugation Unconjugated

Goat Anti-Mouse IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Mouse IgG
Conjugation Unconjugated

Biotin Conjugated Goat Anti-Rabbit IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rabbit IgG
Conjugation Biotin

Rabbit Anti-Mouse IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Mouse IgG
Conjugation Unconjugated

Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Mouse IgG
Conjugation HRP

Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP

Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Goat IgG
Conjugation HRP

Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000-1/250,000.
Immunohistochemistry: 1/500-1/2,500.
Western blot: 1/10,000-1/40,000.
Reactivities Rabbit
Conjugation HRP

Biotin Conjugated Goat Anti-Mouse IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgG
Conjugation Biotin

Rabbit Anti-Goat IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Goat IgG
Conjugation Unconjugated

Biotin Conjugated Goat Anti-Rabbit IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rabbit IgG
Conjugation Biotin

Rabbit IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions
IF Microscopy.
ELISA: 1/10,000-1/60,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2500.
Immunoprecipitation.
Reactivities Rabbit

Monkey IgG (Fc specific) goat polyclonal antibody, FITC

Applications Can be used:
• In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates.
• To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilution: 1/20-1/80.
Reactivities Monkey
Conjugation FITC

Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse

Human IgG (Fc specific) goat polyclonal antibody, HRP

Applications ELISA: 1/3000.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation HRP

Human IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1-5 µg/ml.
Immunohistochemistry on Frozen Sections.
Reactivities Human

Human IgG3 sheep polyclonal antibody, HRP

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/100-1/200.
Reactivities Human
Conjugation HRP

Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for ELISA, Immunofluorescence Microscopy.
Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Conjugation FITC

Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western blot: 1/2,000-1/10,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse

Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/3,000-1/25,000.
Western blot: 1/1,000-1/4,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP

Biotin Conjugated Goat Anti-Mouse IgM

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgM
Conjugation Biotin

Biotin Conjugated Rabbit Anti-Goat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Goat IgG
Conjugation Biotin

Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA : 1:50,000 - 1:300,000
Western Blot : 1:10,000 - 1:30,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Conjugation HRP

Mouse IgG (H+L chain) sheep polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/200,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Human IgE goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Human, Monkey
Conjugation HRP

Mouse IgD (Fc specific) goat polyclonal antibody, Biotin

Applications In Immunocytochemical and Immunohistochemical staining of IgD at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgD isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgD in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/1000.
Reactivities Mouse
Conjugation Biotin

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000 - 1/20000.
Reactivities Rabbit
Conjugation Biotin

Goat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/50,000-1/100,000. 
Western Blot: 1/5,000-1/20,000. 
Immunohistochemistry: 1/1,000-1/5,000. 
Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Conjugation HRP

Rabbit IgG (H+L chain) goat polyclonal antibody, FITC, Lyophilized

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluoresence: 1/1,000-1/5,000.
Fluorescent Western Blot.
Reactivities Rabbit
Conjugation FITC

Mouse IgG (Fc specific) goat polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunofluorescence: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation FITC

Rabbit Anti-Human IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Human IgG
Conjugation Unconjugated

Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/100,000.
Western blot: 1/500-1/3,000.
Immunohistochemistry: 1/500-1/1,500.
Conjugation HRP

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Immunocytochemistry.
Immunohistochemistry.
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain) goat polyclonal antibody, Biotin, Lyophilized

Applications Western Blot: 1/5,000-1/20,000 for Western blots.
ELISA: 1/50,000-1/200,000.
Immunohistochemistry: 1/1,000-1/5,000.

Reactivities Rabbit
Conjugation Biotin

Mouse IgG (Fc specific) goat polyclonal antibody, HRP

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000
Western blot: 1/1,000-1/5,000
Flow Cytometry: 1/500-1/2,500
Reactivities Mouse
Conjugation HRP

Canine IgG2 rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Western Blot: 1:1000 - 1:10,000.
Immunohistochemistry on frozen sections: 1:200 - 1:2000.
Reactivities Canine

Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rat IgG
Conjugation HRP

Mouse IgG1 goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP

Human IgA (Secretory component) goat polyclonal antibody, Biotin

Applications This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Human body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Reactivities Human
Conjugation Biotin

Human IgA (Secretory component) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in human milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/6000.
Reactivities Human
Conjugation HRP

Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells.
Combinations of isotype-specific reagents should be used instead for this purpose.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommneded Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000.
Reactivities Monkey
Conjugation HRP

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE

Human IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.

Rabbit IgG (H+L chain) goat polyclonal antibody, Texas Red, Lyophilized

Applications Suitable for immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1000 - 1/5000.
Reactivities Rabbit
Conjugation Texas Red

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/40,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP

Mouse IgG (Fc specific) goat polyclonal antibody, Biotin

Applications ELISA: 1/20,000-1/100,000
Western blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Duck IgM (Fc specific) goat polyclonal antibody, HRP

Applications Suitable for use in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of Duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/3,000.
Immunohistochemistry and Cytochemistry: 1/50-1/250.
Note: This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation HRP