Secondary Antibodies

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Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612HRP

USD 280.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, HRP

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000
Western blot: 1/1,000-1/5,000
Flow Cytometry: 1/500-1/2,500
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG F(c) fragment
Canine IgG2 Rabbit Polyclonal Antibody
SP2131P

USD 250.00

5 Days

Canine IgG2 rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Western Blot: 1:1000 - 1:10,000.
Immunohistochemistry on frozen sections: 1:200 - 1:2000.
Reactivities Canine
Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)
TA130038

USD 185.00

3 Weeks

Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rat IgG
Conjugation HRP
Immunogen Rat IgG, whole molecule
Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 25-1 (HP 6207)]
AM20254BT-N

USD 680.00

2 Weeks

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Biotin

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin
Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258AF-N

USD 450.00

In Stock

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Mouse IgG1 Goat Polyclonal Antibody
AP05384AP-N

USD 365.00

5 Days

Mouse IgG1 goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG1 paraproteins.
Human IgA (Secretory component) Goat Polyclonal Antibody
AP21476BT-N

USD 410.00

2 Weeks

Human IgA (Secretory component) goat polyclonal antibody, Biotin

Applications This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Human body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Reactivities Human
Conjugation Biotin
Immunogen Purified free Human Secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgA (Secretory component) Goat Polyclonal Antibody
AP21476HR-N

USD 550.00

2 Weeks

Human IgA (Secretory component) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in human milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/6000.
Reactivities Human
Conjugation HRP
Immunogen Secretory component can be present in Human secretions bound to secretory IgA (sIgA) and in free form.
Free Human secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fab specific) Rabbit Polyclonal Antibody
AP31444HR-N

USD 550.00

2 Weeks

Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells.
Combinations of isotype-specific reagents should be used instead for this purpose.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommneded Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal Fab of polyclonal Rhesus Monkey IgG.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgG (gamma chain specific) Rabbit Polyclonal Antibody
R1336P

USD 220.00

3 Days

Human IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Immunogen Human IgG gamma chain heavy chain.
Rabbit IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
R1454HRP

USD 355.00

3 Days

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/40,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Rabbit IgG whole molecule.
Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612B

USD 265.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, Biotin

Applications ELISA: 1/20,000-1/100,000
Western blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin
Immunogen Anti-mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat.
Canine IgE Mouse Monoclonal Antibody [Clone ID: E6-2A1]
SM1498P

USD 495.00

5 Days

Canine IgE mouse monoclonal antibody, clone E6-2A1, Purified

Applications Western Blot.
ELISA: The antibody may be used as a Capture antibody in ELISA in combination with SM1499B as detecting antibody.
Reactivities Canine
Human IgE Mouse Monoclonal Antibody [Clone ID: NI 307]
AM20251FC-N

USD 680.00

2 Weeks

Human IgE mouse monoclonal antibody, clone NI 307, FITC

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
Reactivities Human
Conjugation FITC
Human IgA (Secretory component) Mouse Monoclonal Antibody [Clone ID: NI 194-4 (A89-039)]
AM20261HR-N

USD 680.00

2 Weeks

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), HRP

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/20 upwards.  
ELISA: form 1/50 upwards.  
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP
Duck IgM (Fc specific) Goat Polyclonal Antibody
AP21447HR-N

USD 550.00

2 Weeks

Duck IgM (Fc specific) goat polyclonal antibody, HRP

Applications Suitable for use in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of Duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/3,000.
Immunohistochemistry and Cytochemistry: 1/50-1/250.
Note: This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgE (Fc specific) Goat Polyclonal Antibody
AP21480HR-N

USD 410.00

2 Weeks

Human IgE (Fc specific) goat polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000.
Reactivities Human
Conjugation HRP
Immunogen Purified monoclonal IgE isolated from Human serum.
Feund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2b (subclass specific) Goat Polyclonal Antibody
AP21491BT-N

USD 410.00

2 Weeks

Rat IgG2b (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rat
Conjugation Biotin
Immunogen Pools of purified homogenous IgG2b isolated from rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fc specific) Goat Polyclonal Antibody
AP31438HR-N

USD 550.00

2 Weeks

Monkey IgG (Fc specific) goat polyclonal antibody, HRP

Applications Dot blot.
Immunoblotting. 
ELISA.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
This antibody can be used:
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure
In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids.
This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histo- and Cytochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fab specific) Rabbit Polyclonal Antibody
AP31444AF-N

USD 470.00

2 Weeks

Monkey IgG (Fab specific) rabbit polyclonal antibody, Azide Free

Applications Can be used for indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Monkey
Immunogen Purified Fab from normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fab specific) Rabbit Polyclonal Antibody
AP31444BT-N

USD 550.00

2 Weeks

Monkey IgG (Fab specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining to identify and measure free or Ig bound subunits of both kappa and lambda type at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified Fab from normal IgG isolated from pooled Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM Goat Polyclonal Antibody
R1250HRP

USD 340.00

3 Days

Monkey IgA + IgG + IgM goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Monkey IgG IgA and IgM whole molecules in goat.
Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1398P

USD 230.00

3 Days

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified

Applications Application(s): suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s):
ELISA: 1:25,000
Western Blot: 1:1,000 - 1:5,000
Immunohistochemistry: 1:500 - 1:2,500
Immunogen Human IgG F(ab')2 fragment.
Human IgG3 (Hinge Region) Mouse Monoclonal Antibody [Clone ID: HP6050]
SM1026HRP

USD 340.00

5 Days

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP

Applications ELISA: 1/4,000-1/10,000.
Western blotting.
Dot- and Slot-immunoblotting.
Immunohistochemistry on Frozen Sections: 1/10-1/20.
Reactivities Human
Conjugation HRP
Biotin Conjugated Rabbit Anti-Rat IgG
TA130034

USD 125.00

3 Weeks

Biotin Conjugated Rabbit Anti-Rat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rat IgG
Conjugation Biotin
Immunogen Rat IgG, whole molecule
Rabbit Monoclonal Anti-Mouse IgG (gamma-Chain) antibody, clone RM104
TA130054

USD 305.00

5 Days

Rabbit Monoclonal Anti-Mouse IgG (gamma-Chain) antibody, clone RM104

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG gamma
Conjugation Unconjugated
Human IgA2 Mouse Monoclonal Antibody [Clone ID: NI 512 (A89-038)]
AM20247AF-N

USD 450.00

2 Weeks

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Ascites

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 25-1 (HP 6207)]
AM20254HR-N

USD 680.00

2 Weeks

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), HRP

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/100 upwards.
Western blot: from 1/250 upwards.
Reactivities Human
Conjugation HRP
Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]
AM20255PU-N

USD 550.00

2 Weeks

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Purified

Applications To identify the presence of IgG3 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG3, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.  
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Human IgA (Secretory component) Mouse Monoclonal Antibody [Clone ID: NI 194-4 (A89-039)]
AM20261TC-N

USD 680.00

2 Weeks

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), TRITC

Applications To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
Reactivities Human
Conjugation TRITC
Human IgA (IgAc, A1, A2, Secretory) Mouse Monoclonal Antibody [Clone ID: NI 69 and NI 184+NI 69-11+NI 512+NI 194]
AM31706AF-N

USD 1,060.00

2 Weeks

Human IgA (IgAc, A1, A2, Secretory) mouse monoclonal antibody, clone NI 69 and NI 184+NI 69-11+NI 512+NI 194, Ascites

Applications Can be used to identify the presence of IgA and secretory subclasses in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting. 
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards. 
Western blotting: 1/2000-1/10000.
Reactivities Human
Human IgG, IgA, IgM, IgD, IgE Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307]
AM31707AF-N

USD 1,060.00

2 Weeks

Human IgG, IgA, IgM, IgD, IgE mouse monoclonal antibody, clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307, Ascites

Applications Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards.
Western blotting: 1/2000 Upwards.
Reactivities Human
Chicken IgM (Fc specific) Goat Polyclonal Antibody
AP21443HR-N

USD 410.00

2 Weeks

Chicken IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Chicken
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Duck IgM (Fc specific) Goat Polyclonal Antibody
AP21447SU-N

USD 270.00

2 Weeks

Duck IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in duck serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Duck IgM from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Precipitin titre: 1/16 when tested against pooled normal duck serum in agar-block immunodiffusion titration.
Reactivities Duck
Immunogen Highly purified normal IgM isolated from pooled Duck serum.
Freund's complete adjuvant is used in the first step of the immunization procedure.
Guinea Pig IgG2 (subclass specific) Goat Polyclonal Antibody
AP21454BT-N

USD 550.00

2 Weeks

Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/2000.
Reactivities Guinea Pig
Conjugation Biotin
Immunogen Purified IgG2 isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Guinea Pig IgG2 (subclass specific) Goat Polyclonal Antibody
AP21454HR-N

USD 550.00

2 Weeks

Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, HRP

Applications This antibody can be used in Enzyme Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,0000.
Reactivities Guinea Pig
Conjugation HRP
Immunogen Purified IgG2 isolated from Guinea Pig serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Guinea Pig IgM (Fc specific) Goat Polyclonal Antibody
AP21455BT-N

USD 550.00

2 Weeks

Guinea Pig IgM (Fc specific) goat polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/5000.
Reactivities Guinea Pig
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Guinea Pig IgM (Fc specific) Goat Polyclonal Antibody
AP21455HR-N

USD 550.00

2 Weeks

Guinea Pig IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used for Enzyme-Immunocytochemical and immunohistochemical detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Guinea Pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Guinea Pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/400-1/4000.
Reactivities Guinea Pig
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled Guinea Pig serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgD (Fc specific) Goat Polyclonal Antibody
AP21479BT-N

USD 410.00

2 Weeks

Human IgD (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgD in human serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Performance testing. Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Human
Conjugation Biotin
Immunogen Highly purified monoclonal IgD isolated from Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgD (Fc specific) Goat Polyclonal Antibody
AP21479HR-N

USD 410.00

2 Weeks

Human IgD (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in nonisotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/10,000.
Reactivities Human
Conjugation HRP
Immunogen Purified polyclonal IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgD (Fc specific) Goat Polyclonal Antibody
AP21481AF-N

USD 550.00

2 Weeks

Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Mouse
Immunogen Purified polyclonal and monoclonal IgD isolated from BALB/C and C57BL Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482SU-N

USD 270.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgE in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
Antibody titre: Precipitin titre 1/32 when tested against monoclonal IgE containing mouse serum in agarblock immunodiffusion titration.
Reactivities Mouse
Immunogen Highly purified monoclonal IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgE (Fc specific) Goat Polyclonal Antibody
AP21483BT-N

USD 410.00

2 Weeks

Rat IgE (Fc specific) goat polyclonal antibody, Biotin

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Rat
Conjugation Biotin
Immunogen Purified homogenous IgE isolated from Rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgE (Fc specific) Goat Polyclonal Antibody
AP21483HR-N

USD 550.00

2 Weeks

Rat IgE (Fc specific) goat polyclonal antibody, HRP

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Reactivities Rat
Conjugation HRP
Immunogen Purified homogenous IgE isolated from Rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485AF-N

USD 550.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Immunogen Purified homogenous IgE isolated from Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485BT-N

USD 550.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgE isolated from mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485HR-N

USD 550.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgE isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgM (Fc specific) Goat Polyclonal Antibody
AP21488BT-N

USD 550.00

2 Weeks

Rabbit IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/10000.
Reactivities Rabbit
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgM (Fc specific) Goat Polyclonal Antibody
AP21488HR-N

USD 550.00

2 Weeks

Rabbit IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled Rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgA (Fc specific) Goat Polyclonal Antibody
AP21489BT-N

USD 550.00

2 Weeks

Rabbit IgA (Fc specific) goat polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100 - 1/1000.
Reactivities Rabbit
Conjugation Biotin
Immunogen Purified polyclonal IgA isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.