E1B (NC_001460) Virus Tagged ORF Clone

CAT#: VC100255

Myc-DDK-tagged ORF clone of viral ORF for E1B gene product [Human adenovirus A], codon optimized for human cell expression, NP_040911

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100255 represents NCBI reference of NP_040911 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGAGACGGTTCTGCAGAGTTTTCAGAGTGTACGACAACTGCTGCAATATACCTCAAAGAATA
  CATCAGGATTCTGGAGATATCTTTTCGGATCCACCCTCTCCAAAGTGGTGAACCGCGTCAAGGAAGACTA
  CCGGGAAGAGTTCGAGAATATACTGGCAGATTGCCCCGGACTGCTGGCTAGTCTGGACCTGTGCTACCAC
  CTCGTGTTCCAGGAAAAGGTGGTGAGGAGCCTGGATTTCTCAAGCGTTGGACGAACTGTCGCCTCCATCG
  CCTTCCTCGCGACTATTCTTGATAAGTGGTCCGAAAAGTCCCACCTGTCATGGGATTACATGCTGGATTA
  CATGTCTATGCAATTGTGGAGGGCATGGCTGAAGCGGCGGGTGTGTATTTATAGCCTCGCAAGGCCCCTC
  ACGATGCCACCACTCCCCACACTGCAGGAAGAGAAGGAAGAGGAACGGAACCCTGCGGTCGTAGAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100255 representing NP_040911
  Red=Cloning sites Green=Tags
  
  MELETVLQSFQSVRQLLQYTSKNTSGFWRYLFGSTLSKVVNRVKEDYREEFENILADCPGLLASLDLCYH
  LVFQEKVVRSLDFSSVGRTVASIAFLATILDKWSEKSHLSWDYMLDYMSMQLWRAWLKRRVCIYSLARPL
  TMPPLPTLQEEKEEERNPAVVEK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 489 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040911
• RefSeq ORF: 489
• Locus ID: 1460854
• MW: 19.1 kDa