L3 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100269

Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040925

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100269 represents NCBI reference of NP_040925 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGCTCAGAGCAGGAACTCACTGCCATAGTGCGGGACCTGGGCTGCGGACCTTATTTTCTTGGTA
  CATTTGATAAGCGCTTTCCAGGTTTTGTCTCACGCGATAGACTGAGTTGCGCAATAGTCAACACCGCTGG
  GCGCGAGACTGGCGGGGTGCACTGGCTGGCCTTCGGCTGGAACCCAAAAAGCCACACCTGCTATCTGTTT
  GACCCCTTTGGCTTTTCTGATCAAAGGTTGAAGCAGATCTACCAGTTTGAGTACGAGTCTCTTCTGAGAA
  GGTCCGCCCTCGCTGCAACAAAAGACAGATGTGTGACACTCGAAAAGTCTACACAAACAGTCCAAGGCCC
  CTTCTCTGCCGCATGCGGGCTGTTTTGTTGTATGTTCCTGCACGCTTTCACCCACTGGCCGGACCATCCG
  ATGGACAAGAACCCGACTATGGATTTGCTTACCGGGGTGCCAAATTGCATGCTCCAGAGCCCACAGGTGG
  TAGGGACATTGCAACGCAATCAGAACGAGCTGTATAAATTCCTTAACAATCTCTCCCCCTACTTCAGGCA
  CAATCGCGAGAGGATCGAAAAGGCCACCTCTTTTACCAAAATGCAAAACGGACTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100269 representing NP_040925
  Red=Cloning sites Green=Tags
  
  MGSSEQELTAIVRDLGCGPYFLGTFDKRFPGFVSRDRLSCAIVNTAGRETGGVHWLAFGWNPKSHTCYLF
  DPFGFSDQRLKQIYQFEYESLLRRSALAATKDRCVTLEKSTQTVQGPFSAACGLFCCMFLHAFTHWPDHP
  MDKNPTMDLLTGVPNCMLQSPQVVGTLQRNQNELYKFLNNLSPYFRHNRERIEKATSFTKMQNGLK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 618 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040925
• RefSeq ORF: 618
• Locus ID: 1460858
• MW: 23.5 kDa