L1 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100262

Myc-DDK-tagged ORF clone of viral ORF for L1 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040918

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100262 represents NCBI reference of NP_040918 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCGGTACTGAGGCAGATGCGGCCACAGCCCCGGGCCACAACCGCCTCTGCCGCCGTTGCACTGA
  GTGGCAGTGGCGAGCAAGAAGAACCTCAGTGCCCAACACTTGAATTGGAGGAAGGTGAAGGCATCGCAAG
  GCTGGGTGCCCATTCCCCGGAGAGACACCCCCGGGTACAACTCGCACGGGATTCCAGAGTCGCATTTGTG
  CCGAGACAGAATATGTTCCGCGACAACTCCGGGGAGGAGGCTGAGGAAATGCGGGATTGTCGGTTCCGGG
  CCGGCAGGGAGCTGCGCAGAGGCTTCAATCGCGAGAGACTGCTGCGGGAGGAGGACTTCGAGCCGGACGA
  GCACTCAGGGATATCATCCGCACGAGCTCACGTGAGCGCCGCCAATCTGGTTACAGCTTACGAGCAAACT
  GTCACTGAAGAACGGAATTTCCAAAAAAGCTTCAACAACCATGTTAGGACACTGATAGCCCGGGAGGAAG
  TCGCCATTGGGTTGATGCACCTGTGGGACTTTGTGGAGGCGTACGTCCACAACCCCGCGTCAAAGCCACT
  CACAGCTCAGCTGTTCTTGATCGTCCAGCATTCTAGGGACAACGAAACCTTCAGAGACGCCATGCTGAAC
  ATCGCCGAACCGCAGGGGCGGTGGCTCCTGGACCTTATAAATATCCTGCAGTCAATTGTTGTTCAAGAGC
  GATCTCTTAGCCTCGCTGACAAAGTCGCCGCAATCAATTATTCTATGCTCTCCCTGGGAAAATTCTACGC
  TCGCAAAATTTATAAATCCCCCTATGTGCCTATAGATAAGGAGGTCAAAATTGACTCATTCTACATGCGG
  ATGGCTTTGAAAGTTCTGACACTCTCCGATGACCTGGGAGTGTATCGGAACGACCGGATTCACAAAGCAG
  TGAGTGCCTCCCGCAGGCGCGAACTGAGCGATAAGGAGCTCATGCATTCACTGCAGCGCGCACTGACAGG
  GGCTGGGACTGAAGACGAGTCATTCTTCGATATGGGCGCCGATTTGCGGTGGCAGCCCAGTGCCCGAGCA
  CTGGAAGCCGCTGGTGTCGCCTCCGCCGACGTTACCGGCGACGACGACGACGAGGATCAGTACGAAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100262 representing NP_040918
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPQPRATTASAAVALSGSGEQEEPQCPTLELEEGEGIARLGAHSPERHPRVQLARDSRVAFV
  PRQNMFRDNSGEEAEEMRDCRFRAGRELRRGFNRERLLREEDFEPDEHSGISSARAHVSAANLVTAYEQT
  VTEERNFQKSFNNHVRTLIAREEVAIGLMHLWDFVEAYVHNPASKPLTAQLFLIVQHSRDNETFRDAMLN
  IAEPQGRWLLDLINILQSIVVQERSLSLADKVAAINYSMLSLGKFYARKIYKSPYVPIDKEVKIDSFYMR
  MALKVLTLSDDLGVYRNDRIHKAVSASRRRELSDKELMHSLQRALTGAGTEDESFFDMGADLRWQPSARA
  LEAAGVASADVTGDDDDEDQYED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040918
• RefSeq ORF: 1119
• Locus ID: 1460848
• MW: 42.3 kDa