IVa2 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100258

Myc-DDK-tagged ORF clone of viral ORF for IVa2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040914

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100258 represents NCBI reference of NP_040914 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCCCTGCAGGTCTACTGGCCGGAGGCATGCATTGCAGCATCAGCCACCTCAATTTAAGGCACATA
  CTGGACAGAGATCAACACGATCAGCCTCCATCCACCGAGATCGCAACAATCCCGATGCACACATTACTAG
  TCTGGAAGGACAAAATCCCAGGCCCTTCGGATGCAGTCCACCTCGGTCCCTGCAGCAGCAGCCGGCCAAA
  CCTCCACAGCGAGGAAGTTTGCTGGATCGGGATGGCATCGAAAATATTACGGAGCTGTGGGACCGCCTCC
  AGTTGCTGAAGCAGACATTGGATCACATGCCCATGGCTGACGGCCTCAAGCCACTGAAAAACTTCAAGAG
  TCTGCAGGAATTGCTGTCCCTCGGCGGAGAAAGACTGCTTGGGGAGTTGGCCAAGCAAAACATTAGAGTT
  CGCCAAATGATGAACGAGGTTGCTCCGTTGCTGCACGAAGATGGATCCTGTACCTCACTGAACTATCATC
  TGCAGCCTGTTATTGGGGTGATTTACGGTCCAACTGGCTGTGGTAAATCTCAGCTGCTTAGGAATTTGCT
  GAGCGCACAATTGGTGACACCCGCTCCAGAAACCGTCTTCTTTATCGTTCCACAAGTGGATATGATACCT
  CCCAGCGAGATTAAGAGTTGGGAAATGCAAATTTGCGAGGGTAACTATGTCCCCGGACCCGAGGGCACTA
  TAATCCCTCAGTCTGGCACGCTGTGCCCGAAGTTTGTGAAACTGAGTTATGATGACCTGACCCTTGATCA
  CAATTATGATGTGTCCGATCCTGAGAACATCTTCGCTCAAGCCGCGGCAAGAGGCCCTATCGCTATTATT
  ATGGACGAGTGTATGGAAAACCTTGGCAGTCATAAGGGCGTGTCCAAGTTCTTTCACGCATTCCCGTCAA
  AATTGCATGACAAATTTCCCAAATGTACCGGCTACACAGTATTTGTGGTGCTGCACAACATGAATCCAAG
  GAGAGACCTGGCCGGGAACATCGCCAATCTCAAGATCCAGTCCAAACTTCATATTATGTCTCCGCGCATG
  CATCCTACACAGCTTAATCGATTTATCAACACCTACACGAAAGGTCTGCCCCTGGCTATCTCTTTGCTGC
  TCAAAGATATCTTCAACCATCACGCCCAGCGCTGTTGCTATGACTGGATCATTTACAATACTAACCCCGA
  GCACGAAGCCCTCCAATGGAGTTACCTGCACCCTAAGGATGGGCTGATGCCCATGTATCTTAACATACAG
  GCCCATCTGTATAAAATTCTCGAGCACATTCATCGGGTCTTGAACGACAGAGAGAGATGGTCCAGGGCCT
  ATCACGTGCGGAAGAACAAGTACCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100258 representing NP_040914
  Red=Cloning sites Green=Tags
  
  MLPCRSTGRRHALQHQPPQFKAHTGQRSTRSASIHRDRNNPDAHITSLEGQNPRPFGCSPPRSLQQQPAK
  PPQRGSLLDRDGIENITELWDRLQLLKQTLDHMPMADGLKPLKNFKSLQELLSLGGERLLGELAKQNIRV
  RQMMNEVAPLLHEDGSCTSLNYHLQPVIGVIYGPTGCGKSQLLRNLLSAQLVTPAPETVFFIVPQVDMIP
  PSEIKSWEMQICEGNYVPGPEGTIIPQSGTLCPKFVKLSYDDLTLDHNYDVSDPENIFAQAAARGPIAII
  MDECMENLGSHKGVSKFFHAFPSKLHDKFPKCTGYTVFVVLHNMNPRRDLAGNIANLKIQSKLHIMSPRM
  HPTQLNRFINTYTKGLPLAISLLLKDIFNHHAQRCCYDWIIYNTNPEHEALQWSYLHPKDGLMPMYLNIQ
  AHLYKILEHIHRVLNDRERWSRAYHVRKNKYQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 1356 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040914
• RefSeq ORF: 1356
• Locus ID: 1460856
• MW: 51.5 kDa