L2 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100265

Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040921

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100265 represents NCBI reference of NP_040921 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGAAACGCAAGTTCAAGGAGGAACTGCTCCAGGCTCTGGCTCCTGAGATTTACGGACCCTCCGATA
  ACCTGACCAAGAGAGATATTAAGCACGTAAAAAAGAGGGAGAAGAAGGAAGAAGAGGTTGCGGCCGCATC
  TGCTGATGGGGTGGAGTTCGTCCGGAGCTTCGCACCTAGAAGGCGAGTGCAGTGGAAAGGCAGACAGGTG
  AAGCGGATTCTGAGGCCCGGGACAACGGTGGTGTTTTCCCCTGGCGAGAGAACTATCATGCGCCCCCTCA
  AAAGGGAATACGACGAGGTTTACGCTGACGACGACATCCTTGAGCAGGCGGCCCAGCAGACTGGGGAATT
  TGCCTACGGCAAAAAAGGCAGGTACGGCGACAAGATTGCCATCCCTTTGGACGAAGGGAACCCAACTCCC
  TCCCTGAAAGCTGTCACACTGCAACAAGTGCTCCCAGTACTGGGACCAAGCGAGGAAAAGCGCGGAATAA
  AAAGAGAAGCTATGGACGAGTTGCAGCCTACTATGCAGCTCATGGTGCCCAAGCGCCAGAAACTCGAGGA
  CGTGCTCGAACACATGAAGGTGGATCCCTCCGTCCAGCCTGATGTCAAAGTTAGGCCTATAAAAAAAGTG
  GCCCCCGGCCTCGGTGTGCAGACAGTGGACATACAGATCCCGGTGCAAACTGCTTTGGGGGAAACCATGG
  AGATTCAGACATCACCCATTAAGACTACAGTGAATGCATCTGTTCAGACCGATCCCTGGTACCCTCCTGT
  GCTTAGCACCAAGAAGAAGCGACACTATAGGCAGACTAGTTCACTGCTGCCTGACTATGTTCTGCACCCC
  TCCATAGTGCCAACACCAGGATACAGGGGCACCACGTTCCAAAGACGCGCTACTGCTCCCAGCCGACGCC
  GAGGCCCCTCTCGGAGACGCAGACGCCGAAAGGCCACCCTGGCTCCTGCCGCTGTAAGGAGAGTCGTCCA
  ACGGGGCAGAACCCTCATTCTCCCTAGTGTGAGGTATCACCCATCCATACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100265 representing NP_040921
  Red=Cloning sites Green=Tags
  
  MTKRKFKEELLQALAPEIYGPSDNLTKRDIKHVKKREKKEEEVAAASADGVEFVRSFAPRRRVQWKGRQV
  KRILRPGTTVVFSPGERTIMRPLKREYDEVYADDDILEQAAQQTGEFAYGKKGRYGDKIAIPLDEGNPTP
  SLKAVTLQQVLPVLGPSEEKRGIKREAMDELQPTMQLMVPKRQKLEDVLEHMKVDPSVQPDVKVRPIKKV
  APGLGVQTVDIQIPVQTALGETMEIQTSPIKTTVNASVQTDPWYPPVLSTKKKRHYRQTSSLLPDYVLHP
  SIVPTPGYRGTTFQRRATAPSRRRGPSRRRRRRKATLAPAAVRRVVQRGRTLILPSVRYHPSIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040921
• RefSeq ORF: 1032
• Locus ID: 1460868
• MW: 39.1 kDa