L4 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100283

Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640219

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100283 represents NCBI reference of YP_002640219 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCTAAAGCGGCTAAACAAGCTATCGCCCAGCAGCGATCTAAAAAGCAGCAGCAGCGCCAAGAGA
  TCTGGGAGGAGGAGAGTTGGGAGTCACAGGCCGAGGACGAGGTGGAGGACCTGGAGGAGTGGGAGGAGGA
  AGAGGCGGATAGCCTGGACGAGGACCCCGAAGAGGAAGAGGAGGGCTCAAAAGACGGCGCTACTGCAGCT
  AAGCCAAGTTTGTCAACAAAACCTTCCCCTATGAAACCCGCAGTCTCTAAGTCCCAGAAAGCCAACCGCC
  GATGGGACACCATAGAAACTTCTGCTGCCAACCTGGGAAAGAACCGCAAGCAGGCTAGGAGAGGCTATTG
  CTCTTGGCGCGCCCACCAGTCCAACATAGTCGCCTGTTTCCAGCACTGCGGAGGAAATATTTCTTTTGCC
  AGAAGATATCTGCTCTACCACGACGGGGTGGCAATACCGCGAAACGTCCTTCATTACTACAGGCACCTGT
  ATAGCCCATTTGAAGAACTTGACAAGGAACCAACTTGTAATAGCCAGGCTGCACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100283 representing YP_002640219
  Red=Cloning sites Green=Tags
  
  MSPKAAKQAIAQQRSKKQQQRQEIWEEESWESQAEDEVEDLEEWEEEEADSLDEDPEEEEEGSKDGATAA
  KPSLSTKPSPMKPAVSKSQKANRRWDTIETSAANLGKNRKQARRGYCSWRAHQSNIVACFQHCGGNISFA
  RRYLLYHDGVAIPRNVLHYYRHLYSPFEELDKEPTCNSQAAH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 546 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, YP_002640219
• RefSeq ORF: 546
• Locus ID: 1460842
• MW: 20.9 kDa