L4 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100271

Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040927

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100271 represents NCBI reference of NP_040927 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGATCTGGAACCTCAAGAATCCCTGACTGCGCCCACGGCTCCTGCGATCGGAGCAACAGCTGTGA
  TGGAGAAAGACAAGTCTCTGCTCATCCCACAGGATGCACCCGTGGAGCAGAATCTGGGGTATGAAACTCC
  CCCTGAGGAGTTTGAAGGCTTCCTCCAGATCCAAAAGCAGCCCAATGAACAGAACGCAGGGCTCGAGGAC
  CATGACTATCTGAATGAGGGTGATGTGTTGTTTAAACATCTGCAGAGACAGAGCACCATTGTGAGGGACG
  CCATTTCAGACCGGTCCTCAATCCCAGTTAGCATCGCAGAACTCTCATGTATCTATGAGCGCAATCTCTT
  CAGCCCTCGCGTGCCACCAAAGAGGCAGGCTAACGGGACTTGTGAGCCTAATCCCAGGCTTAATTTCTAC
  CCCGTGTTTGCCGTTCCCGAGGCTCTGGCCACTTACCATATCTTCTTTAAGAACCACAAGATTCCGCTCA
  GCTGTAGAGCAAATAGGTCTCGCGCGGACGAACTCCTGGCGCTGCGCGCGGGTGCTAGCATCCCTGGAAT
  AGTTTCACTTGAGGAGGTGCCTAAAATTTTTGAAGGCCTTGGACGCGACGAAAAACGCGCCGCAAACGCT
  CTTCAGAAAGAAAATGAACAGAATCACCACGGGAACTCAGCACTCATAGAACTTGAAGGAGACAACGCCC
  GATTGGCAGTACTGAAGAGGAACATCGAGGTTACACACTTTGCATACCCTGCCGTGAACCTCCCACCTAA
  GGTAATGTCAGCCGTGATGAACCAGTTGCTCATCAAGAGGGCGCAGCCTATCGATAAAGACGCGAACCTG
  CAGGACCCGGAAGCCACAGACGATGGCAAACCCGTCGTTTCAGACGAGCAGCTGACGAAGTGGTTGGGCA
  CCGACAATTCTAATGAGCTTCAGCAGCGACGGAAATTGATGATGGCAGCGGTGCTTGTGACAGTGGAGCT
  CGAATGTATGCATCGGTTTTTCAGCGATATAACTACACTGCGCAAGATCGAGGAGTGTCTGCATTATACT
  TTTCGGCATGGCTACGTTAGGCAGGCGTGCAAGATCTCAAACGTCGAGCTTTCTAATTTGGTCTCTTATA
  TGGGTATTCTGCACGAAAATCGACTGGGACAGAATGTCCTTCATTCAACTTTGAGGGATGAAGCCCGCCG
  CGACTACGTCAGAGACTGCATTTACCTGTTTCTGCTCCACACATGGCAGACTGGTATGGGAGTTTGGCAG
  CAGTGCCTCGAGGAAAAGAACCTTAGGGAACTCAACAAGTTGCTCGATCGCGCTTTGAAGAGTCTGTGGA
  CTGGATTCGATGAAAGAACGGTAGCTGCAGAACTCGCAGATATTATTTTCCCCGAGAGGCTGATGATTAC
  CCTTCAGAACGGCCTTCCAGACTTCATGTCCCAGAGCATGCTGCATAATTACCGGAGTTTTATCCTGGAG
  CGAAGTGGCATGTTGCCTTCAATGTGCTGTGCCTTGCCTAGTGATTTTGTGCCAATTTACTTTCGCGAAT
  GCCCGCCCCCTCTGTGGAGCCACTGCTACCTTCTGAGACTGGCTAATTACCTCGCTTATCACTCAGATTT
  GATGACTGACAGTAGCGGAGAGGGACTCATGGAGTGTCACTGTCGGTGTAACCTGTGCACCCCACACCGC
  TCACTCGTGTGCAACACAGAACTGCTGTCCGAGTCCCAGGTCATCGGTACGTTCGAAATGCAGGGTCCTC
  AGAGCGATTCTAACTTTACCACTAATCTGCGGCTTACACCAGGATTGTGGACCAGTGCTTATCTTCGGAA
  ATTCGAACCCCAGGACTATCATGCACACAGCATCAATTTTTACGAGGATCAGTCAAAACCTCCGAAGGCT
  CCCCTTACTGCTTGCGTGATTACACAAGGAAAGATTCTGGCTCAGCTGCACGCCATAAAGCAAGCCCGCG
  AGGAGTTTCTGCTCAAGAAGGGGCATGGCGTGTATCTCGATCCCCAGACCGGAGAAGAACTGAACCTTCC
  TTCTCCTCTGTGCGCCACAGCAAGTCCACACTCTCAGCATGTCCCTGAAAGCCGCAAAACCGGCTACTGT
  GCCGCGACACTTAAAGAGACAGCAGCGACTGCAGGCAATCTGGGTGGACGGATACTCGGGGAGTCAGGCC
  GGGGACGCGGTAGAGGACTGGGAAGAATGGGTGGTGGAGGTGGAGGACAACCAAGAAGAGGATCCCGCGG
  AGGCGGAGGTAGGTTTCAAGGAAGAAGCGACAGACGGCAGACAGTTGCGTTCAATCAGGCCCTGAGTAAC
  GAAACCCGGTGTGAGCAAATCAGCGAGTCACAACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100271 representing NP_040927
  Red=Cloning sites Green=Tags
  
  MMDLEPQESLTAPTAPAIGATAVMEKDKSLLIPQDAPVEQNLGYETPPEEFEGFLQIQKQPNEQNAGLED
  HDYLNEGDVLFKHLQRQSTIVRDAISDRSSIPVSIAELSCIYERNLFSPRVPPKRQANGTCEPNPRLNFY
  PVFAVPEALATYHIFFKNHKIPLSCRANRSRADELLALRAGASIPGIVSLEEVPKIFEGLGRDEKRAANA
  LQKENEQNHHGNSALIELEGDNARLAVLKRNIEVTHFAYPAVNLPPKVMSAVMNQLLIKRAQPIDKDANL
  QDPEATDDGKPVVSDEQLTKWLGTDNSNELQQRRKLMMAAVLVTVELECMHRFFSDITTLRKIEECLHYT
  FRHGYVRQACKISNVELSNLVSYMGILHENRLGQNVLHSTLRDEARRDYVRDCIYLFLLHTWQTGMGVWQ
  QCLEEKNLRELNKLLDRALKSLWTGFDERTVAAELADIIFPERLMITLQNGLPDFMSQSMLHNYRSFILE
  RSGMLPSMCCALPSDFVPIYFRECPPPLWSHCYLLRLANYLAYHSDLMTDSSGEGLMECHCRCNLCTPHR
  SLVCNTELLSESQVIGTFEMQGPQSDSNFTTNLRLTPGLWTSAYLRKFEPQDYHAHSINFYEDQSKPPKA
  PLTACVITQGKILAQLHAIKQAREEFLLKKGHGVYLDPQTGEELNLPSPLCATASPHSQHVPESRKTGYC
  AATLKETAATAGNLGGRILGESGRGRGRGLGRMGGGGGGQPRRGSRGGGGRFQGRSDRRQTVAFNQALSN
  ETRCEQISESQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 2346 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040927
• RefSeq ORF: 2346
• Locus ID: 1460842
• MW: 87.7 kDa