E4 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100282

Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_597784

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100282 represents NCBI reference of NP_597784 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGCGATAGACGGTACAGATACCGCCTTGCACCTTATAATAAGTATCAGCTGCCTCCCTGTGAAG
  AGCAGTCCAAGGCCACATTGTCAACATCAGAAAACTCATTGTGGCCTGAATGTAATTCTCTGACCCTGCA
  CAATGACAAGATTATGGACTTGGTCCTGGACGGCGAGTGCCGCTTGAGTGATTGCGCCGGCGAGGGGTTC
  GTAAGTATCACTGATCCGAGATTTGCCCGGAAAGAGACCGTCTGGACCCTGACACCGAAGAATCTTAGTA
  GAAACATTCAAGTTCAGTTGTTTAGTGCCACTAAGGGAGAGCGGGAGGTCTACAAAGTAAAATGGGAAGG
  CGGCTCTCTCACCACCAGAATTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100282 representing NP_597784
  Red=Cloning sites Green=Tags
  
  MQRDRRYRYRLAPYNKYQLPPCEEQSKATLSTSENSLWPECNSLTLHNDKIMDLVLDGECRLSDCAGEGF
  VSITDPRFARKETVWTLTPKNLSRNIQVQLFSATKGEREVYKVKWEGGSLTTRIV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 375 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_597784
• RefSeq ORF: 375
• Locus ID: 1460865
• MW: 14.5 kDa