E4 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100278

Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040934

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100278 represents NCBI reference of NP_040934 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAGGGACCGAAGATACCGATATAGGCTGGCTCCGTACAACAAGTATCAACTGCCACCTTGTGAAG
  AACAAAGTAAGGCAACCCTTAGCACAAGCGAGAACTCACTGTGGCCAGAGTGCAACTCACTGACCCTGCA
  TAACGTATCTGAAGTTAGGGGCATACCCTCATGCGTCGGGTTCACAGTGCTGCAGGAATGGCCAATCCCT
  TGGGATATGATTCTGACTGACTACGAGATGTTTATCCTGAAAAAGTATATGTCTGTGTGTATGTGCTGCG
  CTACAATTAACGTGGAAGTCACACAGCTCCTGCACGGACATGAACGCTGGCTTATCCACTGCCACTGCCA
  GAGGCCTGGATCCCTGCAGTGTATGTCCGCTGGCATGCTGCTGGGACGGTGGTTCAAGATGGCCGTGTAC
  GGAGCCCTCATCAACAAGCGCTGTTTTTGGTACAGAGAGGTTGTCAATCACCTCATGCCAAAGGAGGTTA
  TGTATGTAGGCAGCACATTTGTTCGAGGTAGGCACCTGATCTATTTTAAGATCATGTACGATGGGCACGC
  ATGGCTGGCGCTGGAGAAAGTCTCATTTGGCTGGTCTGCATTCAACTACGGCATCCTGAATAACATGCTG
  GTCTTGTGTTGCGACTATTGTAAAGACCTCTCCGAAATAAGGATGAGATGTTGCGCAAGGCGAACGAGGC
  TCTTGATGTTGAAGGTCGTGCAGGTGATCGCCGAGAATACTGTGCGGCCCCTGAAGCACAGCCGACACGA
  ACGCTATAGGCAGCAGCTGCTGAAGGGACTGATCATGCACCATCGCGCCATCCTGTTCGGCGATTATAAT
  CAGCGCGAAAATCCCTGGGCCGCGGACGGCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100278 representing NP_040934
  Red=Cloning sites Green=Tags
  
  MQRDRRYRYRLAPYNKYQLPPCEEQSKATLSTSENSLWPECNSLTLHNVSEVRGIPSCVGFTVLQEWPIP
  WDMILTDYEMFILKKYMSVCMCCATINVEVTQLLHGHERWLIHCHCQRPGSLQCMSAGMLLGRWFKMAVY
  GALINKRCFWYREVVNHLMPKEVMYVGSTFVRGRHLIYFKIMYDGHAWLALEKVSFGWSAFNYGILNNML
  VLCCDYCKDLSEIRMRCCARRTRLLMLKVVQVIAENTVRPLKHSRHERYRQQLLKGLIMHHRAILFGDYN
  QRENPWAADGH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040934
• RefSeq ORF: 873
• Locus ID: 1460865
• MW: 34.4 kDa