E3 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100274

Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040930

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100274 represents NCBI reference of NP_040930 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCACTGTTCTCCTTATCTTCCTGTGTCTGCCTGTGATCTTTTCTTCATCCACTTTTGCTGCAGTCT
  CCGATCTCGACCCAGAGTGCCTGGCCCCCTTTGCGGTGTACTTGATTTTTACTTTCGTGACAGCTACCTG
  TGTGTGTTCTATTATCACATTGCTGATCACATCACTCCAATTTTTTGACTATTATTATGTCCGGATTGTC
  TATAGGCGGCACCATCCTAGATACCAGAACCCTCAGATCGCCGCTCTCCTGCAACTGCAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100274 representing NP_040930
  Red=Cloning sites Green=Tags
  
  MVTVLLIFLCLPVIFSSSTFAAVSDLDPECLAPFAVYLIFTFVTATCVCSIITLLITSLQFFDYYYVRIV
  YRRHHPRYQNPQIAALLQLQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 273 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040930
• RefSeq ORF: 273
• Locus ID: 1460862
• MW: 10.4 kDa