L5 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100277

Myc-DDK-tagged ORF clone of viral ORF for L5 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040933

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100277 represents NCBI reference of NP_040933 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGTCCCGAACCCAATACGCCGAAGAAACCGAGGAGAACGACGATTTTAATCCCGTCTACCCCT
  TCGATCCCTTCGACACCAGCGACGTGCCATTTGTCACGCCCCCTTTCACAAGTAGCAACGGTCTGCAGGA
  GAAGCCCCCCGGCGTTCTTGCCCTTAATTACAAAGACCCAATCGTTACAGAGAACGGCACACTGACTCTT
  AAGCTCGGCGACGGGATCAAGCTCAACGCTCAGGGACAGCTGACAGCATCTAATAACATCAACGTGCTGG
  AGCCACTGACAAATACAAGTCAGGGGCTTAAACTGAGCTGGTCTGCTCCCCTGGCTGTCAAGGCGTCCGC
  TTTGACACTTAACACTAGGGCTCCTCTCACCACCACAGATGAAAGTCTGGCGCTGATCACAGCGCCCCCA
  ATAACTGTAGAAAGTAGCCGACTTGGCCTCGCCACTATTGCACCGCTGTCATTGGATGGGGGCGGCAATC
  TTGGTCTCAACTTGTCCGCTCCCCTCGATGTGAGCAACAACAATCTGCACCTGACAACAGAGACCCCGCT
  CGTGGTTAATAGCTCCGGTGCCCTCAGTGTTGCGACAGCGGACCCTATAAGCGTGCGGAATAACGCACTC
  ACGCTCCCTACCGCTGATCCTCTGATGGTATCAAGCGACGGCCTGGGCATTAGTGTGACCAGTCCTATCA
  CAGTGATCAATGGCAGCCTGGCCCTGTCTACGACCGCACCTCTGAATTCAACCGGTAGTACCCTGTCTCT
  CAGCGTGGCAAACCCCCTGACAATTAGCCAGGATACGCTCACCGTGTCCACAGGCAACGGCTTGCAGGTC
  AGCGGTAGTCAGCTTGTGACACGGATTGGGGATGGGCTGACGTTCGATAACGGCGTCATGAAAGTCAATG
  TGGCCGGTGGGATGAGGACTTCAGGGGGCAGAATTATCCTCGACGTAAACTACCCCTTCGACGCCAGCAA
  CAACCTGTCACTGCGGAGAGGTCTGGGATTGATCTATAACCAGAGCACTAATTGGAATTTGACCACCGAC
  ATAAGCACTGAAAAAGGTCTGATGTTTTCAGGAAACCAGATCGCTTTGAACGCAGGCCAGGGACTGACCT
  TCAATAACGGCCAGCTGCGAGTGAAGCTGGGGGCGGGGCTTATCTTTGACAGTAACAACAATATAGCTCT
  CGGATCTTCCAGTAACACACCCTACGACCCCTTGACCCTCTGGACTACTCCTGATCCACCTCCCAATTGC
  TCCCTGATCCAGGAACTTGACGCCAAATTGACGTTGTGCCTGACAAAGAACGGTAGCATCGTGAATGGCA
  TTGTGTCTCTGGTGGGTGTTAAAGGCAACCTCTTGAACATCCAGTCTACCACCACTACCGTGGGCGTCCA
  TCTTGTCTTCGACGAGCAGGGTAGGCTTATCACCTCTACCCCTACAGCCCTGGTGCCGCAGGCGTCATGG
  GGATACAGACAAGGGCAATCAGTGTCCACAAATACAGTCACGAACGGGCTGGGATTTATGCCTAATGTTA
  GTGCCTACCCCCGACCCAACGCCTCTGAGGCCAAAAGCCAAATGGTGAGCCTCACCTATCTGCAAGGCGA
  CACAAGCAAGCCAATTACCATGAAGGTGGCTTTCAATGGAATTACCAGCCTCAACGGTTATTCCTTGACC
  TTCATGTGGTCTGGGTTGTCAAACTACATAAACCAGCCATTCAGCACCCCATCTTGTTCTTTTAGCTATA
  TAACACAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100277 representing NP_040933
  Red=Cloning sites Green=Tags
  
  MKRSRTQYAEETEENDDFNPVYPFDPFDTSDVPFVTPPFTSSNGLQEKPPGVLALNYKDPIVTENGTLTL
  KLGDGIKLNAQGQLTASNNINVLEPLTNTSQGLKLSWSAPLAVKASALTLNTRAPLTTTDESLALITAPP
  ITVESSRLGLATIAPLSLDGGGNLGLNLSAPLDVSNNNLHLTTETPLVVNSSGALSVATADPISVRNNAL
  TLPTADPLMVSSDGLGISVTSPITVINGSLALSTTAPLNSTGSTLSLSVANPLTISQDTLTVSTGNGLQV
  SGSQLVTRIGDGLTFDNGVMKVNVAGGMRTSGGRIILDVNYPFDASNNLSLRRGLGLIYNQSTNWNLTTD
  ISTEKGLMFSGNQIALNAGQGLTFNNGQLRVKLGAGLIFDSNNNIALGSSSNTPYDPLTLWTTPDPPPNC
  SLIQELDAKLTLCLTKNGSIVNGIVSLVGVKGNLLNIQSTTTTVGVHLVFDEQGRLITSTPTALVPQASW
  GYRQGQSVSTNTVTNGLGFMPNVSAYPRPNASEAKSQMVSLTYLQGDTSKPITMKVAFNGITSLNGYSLT
  FMWSGLSNYINQPFSTPSCSFSYITQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040933
• RefSeq ORF: 1761
• Locus ID: 1460847
• MW: 61.7 kDa