E3 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100276

Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040931

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100276 represents NCBI reference of NP_040931 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACTGCATTGGTCTTGTTCTTTATGCTTATCCCAGTGTGGGCCTCCAGTTGTCAGCTCCACAAGC
  CCTGGAATTTCCTGGATTGTTACACTAAAGAAACTAATTACATTGGATGGGTGTATGGAATTATGTCAGG
  GCTCGTGTTCGTGTCCAGCGTGGTCTCTCTGCAGCTCTACGCCCGCTTGAACTTTTCTTGGAATAAATAC
  ACAGATGATCTCCCCGAATACCCTAATCCCCAAGACGACTTGCCCCTTAACATTGTGTTTCCCGAACCCC
  CTAGACCGCCTAGCGTCGTTAGCTACTTCAAATTCACCGGGGAGGACGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100276 representing NP_040931
  Red=Cloning sites Green=Tags
  
  MKTALVLFFMLIPVWASSCQLHKPWNFLDCYTKETNYIGWVYGIMSGLVFVSSVVSLQLYARLNFSWNKY
  TDDLPEYPNPQDDLPLNIVFPEPPRPPSVVSYFKFTGEDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 330 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001460.1, NP_040931
• RefSeq ORF: 330
• Locus ID: 1460862
• MW: 12.8 kDa